| Project/Area Number |
15K07893
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
Sasai Yasushi 岐阜薬科大学, 薬学部, 准教授 (60336633)
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| Co-Investigator(Kenkyū-buntansha) |
山内 行玄 松山大学, 薬学部, 准教授 (10461378)
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| Co-Investigator(Renkei-kenkyūsha) |
KONDO Shin-ichi 岐阜薬科大学, 薬学部, 教授 (90240944)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 高分子ブラシ / プラズマ化学 / 細胞培養 / 表面化学 / 細胞培養基板 / プラズマ表面処理 / 原子移動ラジカル重合 / 生体適合性高分子 / タンパク質吸着抑制効果 |
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| Outline of Final Research Achievements |
Highly hydrophilic and non-cell adhesive polymer brush layer was fabricated on a commercial polystyrene dish by surface-initiated atom transfer radical polymerization of sulfobetaine- and phosphobetaine-type zwitterionic methacrylic monomers. Plasma surface treatment was effective to change surface properties of the polymer brushes as the results of plasma-induced reactions. ESR analysis suggested that tertiary end-chain radicals generated by polymer main-chain scission were mainly observed in polymer brushes by plasma-irradiation. In addition, XPS analysis indicated that plasma-irradiation caused rapid de-esterification of methacrylic polymer brushes. By patterning plasma-exposed area on non-cell adhesive zwitterionic methacrylic polymer brushes using a masking sheet, the specific areas which promote the formation of size-controlled spheroid-like cell aggregates were successfully prepared.
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