| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Diagnostic approach of several diseases using non-invasive biological samples (saliva, hair and nail) was performed in this study. The determination of biomarker(s) related to diseases was based on targeted and non-targeted metabolomics by UPLC-MS. The chiral derivatization reagents, DMT-3(S)-Apy and DMT-L-Pro-OSu, were first synthesized for the determination of carboxylic acids and amines, respectively. The opposite enantiomer reagents and the isotopic variants were also synthesized.
The ratio of D/L-lactic acids in the saliva of diabetic patients was higher than that of healthy persons. The ratio of polyamines in saliva was also different between breast cancer patients and healthy persons. Several N-acethyl amino acids in hairs of diabetes were significantly different from healthy subjects. Furthermore, the concentration of uric acid was obviously higher in gout patients. The efficiency of saliva, hair and nail as diagnostic sample was shown in this study.
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