Diagnostic approach of diseases using non-invasive biosamples (saliva, hair and nail)
Project/Area Number |
15K07895
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Physical pharmacy
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Research Institution | University of Shizuoka |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 疾病診断 / 光学活性誘導体化試薬 / 非侵襲的試料 / バイオマーカー探索 / 液体クロマトグラフィー / 質量分析 / 非侵襲的試料解析 / 疾病バイオマーカー / 光学活性代謝物 / バイオマーカー |
Outline of Final Research Achievements |
Diagnostic approach of several diseases using non-invasive biological samples (saliva, hair and nail) was performed in this study. The determination of biomarker(s) related to diseases was based on targeted and non-targeted metabolomics by UPLC-MS. The chiral derivatization reagents, DMT-3(S)-Apy and DMT-L-Pro-OSu, were first synthesized for the determination of carboxylic acids and amines, respectively. The opposite enantiomer reagents and the isotopic variants were also synthesized. The ratio of D/L-lactic acids in the saliva of diabetic patients was higher than that of healthy persons. The ratio of polyamines in saliva was also different between breast cancer patients and healthy persons. Several N-acethyl amino acids in hairs of diabetes were significantly different from healthy subjects. Furthermore, the concentration of uric acid was obviously higher in gout patients. The efficiency of saliva, hair and nail as diagnostic sample was shown in this study.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Diagnostic approach to breast cancer patients based on target metabolomics in saliva by liquid chromatography with tandem mass spectrometry.2016
Author(s)
Takayama T, Tsutsui H, Shimizu I, Toyama T, Yoshimoto N, Endo Y, Inoue K, Todoroki K, Min JZ, Mizuno H, Toyo'oka T.
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Journal Title
Clin Chim Acta.
Volume: 452
Pages: 18-26
DOI
Related Report
Peer Reviewed
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[Journal Article] Towards the chiral metabolomics: Liquid chromatography-mass spectrometry based DL-amino acid analysis after labeling with a new chiral reagent, (S)-2,5-dioxopyrrolidin-1-yl-1-(4,6-dimethoxy-1,3,5-triazin-2-yl)pyrrolidine-2-carboxylate, and the application to saliva of healthy volunteers2015
Author(s)
Toshiki Mochizuki, Takahiro Takayama, Kenichiro Todoroki, Koichi Inoue, Jun Zhe Min, Toshimasa Toyo'oka
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Journal Title
Analytica Chimica Acta
Volume: 875
Pages: 73-82
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Human nails metabolite analysis: A rapid and simple method for quantification of uric acid in human fingernail by high-performanceliquid chromatography with UV-detection2015
Author(s)
Xi-Ling Li, Gao Li, Ying-Zi Jiang, Dongzhou Kang, Cheng Hua Jin, Qing Shi,Toufeng Jin, Koichi Inoue, Kenichiro Todoroki, Toshimasa Toyo'oka, Jun Zhe Min
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Journal Title
Journal of Chromatography B
Volume: 1002
Pages: 394-398
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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