| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The aim of this study is to develop a novel near-infrared activatable fluorescent probe which enables to target macrophages in tumor tissues for early diagnosis of tumor. First, I synthesized an activatable fluorescent probe (P-ICG2) which show the near-infrared fluorescent property only after the lysosomal cathepsin B enzymatically cleaves the peptide chain in the probe. On the other hand, to deliver the probe to tumor tissue effectively, I also prepared PS-modified liposomes which can target macrophages infiltrating into tumor tissues. Then, I encapsulated P-ICG2 into the liposomes and developed a macrophage-targetable, enzyme-specific activatable fluorescent probe (P-ICG2-PS-Lip). P-ICG2-PS-Lip showed the fluorescence in lysosomes of mouse macrophage-like RAW264 cells after taking up into the cells. Furthermore, optical imaging of the solid tumor was succeeded, when P-ICG2-PS-Lip was intravenously injected to KLN205 tumor-bearing mouse and the fluorescence imaging was performed.
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