Development of tumor-targetable, enzyme-specific activatable fluorescent probe for early diagnosis of tumor
| Project/Area Number |
15K07896
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Hamamatsu University School of Medicine (2016-2017)
University of Shizuoka (2015) |
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Principal Investigator |
Shimizu Kosuke 浜松医科大学, 光尖端医学教育研究センター, 准教授 (30423841)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | がんイメージング / リポソーム / アクチベータブル蛍光プローブ / マクロファージ / DDS / カテプシンB / ホスファチジルセリン / イメージング / がん / ターゲティング |
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| Outline of Final Research Achievements |
The aim of this study is to develop a novel near-infrared activatable fluorescent probe which enables to target macrophages in tumor tissues for early diagnosis of tumor. First, I synthesized an activatable fluorescent probe (P-ICG2) which show the near-infrared fluorescent property only after the lysosomal cathepsin B enzymatically cleaves the peptide chain in the probe. On the other hand, to deliver the probe to tumor tissue effectively, I also prepared PS-modified liposomes which can target macrophages infiltrating into tumor tissues. Then, I encapsulated P-ICG2 into the liposomes and developed a macrophage-targetable, enzyme-specific activatable fluorescent probe (P-ICG2-PS-Lip). P-ICG2-PS-Lip showed the fluorescence in lysosomes of mouse macrophage-like RAW264 cells after taking up into the cells. Furthermore, optical imaging of the solid tumor was succeeded, when P-ICG2-PS-Lip was intravenously injected to KLN205 tumor-bearing mouse and the fluorescence imaging was performed.
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Report
(4 results)
Research Products
(18 results)
