| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In this study, utilizing fluorous interaction which is affinity between perfluoroalkyl compounds, an improved method for identification of reactive metabolites by glutathione trapping has been developed. This method includes selective perfluoroalkylation of α-carboxyl group of glutathione-trapped reactive metabolites with fluorous amine reagent through oxazolone chemistry. The obtained derivative could be analyzed selectively and sensitively with LC-MS/MS comparing with conventional methods. The proposed method will be useful for the identification of reactive metabolites in various pharmaceutical product.
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