Development of a redox sensitive liposome for diagnosis and treatment for liver damages

• Project/Area Number: 15K07912
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Sojo University
• Principal Investigator: OKAZAKI Shoko 崇城大学, 薬学部, 講師 (40435152)
• Co-Investigator(Renkei-kenkyūsha): TAKESHITA Keizo 崇城大学, 薬学部, 教授 (70175438)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: レドックス / 酸化ストレス / ニトロキシルラジカル / リポソーム / in vivo ESR / MRI

Outline of Final Research Achievements

We synthesized a nitroxyl radical-binding phospholipid and prepared a redox sensitive liposome. It is difficult to measure the in vivo redox by an in vivo ESR technique using a nitroxyl radical in a disease model having renal impairment, because the half-life of a nitroxyl radical is short. However, we achieved increase of retention in the body of the nitroxyl radical by producing a redox sensitive liposome. This would lead to increase of target diseases of non-invasive redox measurement by ESR and MRI, because difference of probe concentration in a body between healthy and disease model animals would decrease.

Research Products

• [Journal Article] Irradiation of phenolic compounds with ultraviolet light causes release of hydrated electrons (2018)
  • Author(s): Shoko Okazaki, Keizo Takeshita
  • Journal Title: Applied Magnetic Resonance Volume: 印刷中 Issue: 8 Pages: 1-12
  • DOI: 10.1007/s00723-018-0999-9
  • Peer Reviewed
• [Journal Article] Application of a Compact Magnetic Resonance Imaging System with 1.5 T Permanent Magnets to Visualize Release from and the Disintegration of Capsule Formulations <i>in Vitro</i> and <i>in Vivo</i> (2017)
  • Author(s): Keizo Takeshita, Shoko Okazaki, Kyosuke Shinada, Yuma Shibamoto
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 40 Issue: 8 Pages: 1268-1274
  • DOI: 10.1248/bpb.b17-00154
  • NAID: 130005876318
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed / Open Access
• [Journal Article] Surface-deacetylated chitin nanofibers reinforced with a sulfobutyl ether β-cyclodextrin gel loaded with prednisolone as potential therapy for inflammatory bowel disease. (2017)
  • Author(s): Tabuchi R, Anraku M, Iohara D, Ishiguro T, Ifuku S, Nagae T, Uekama K, Okazaki S, Takeshita K, Otagiri M, Hirayama F.
  • Journal Title: Carbohydrate Polymers Volume: 174 Pages: 1087-1094
  • DOI: 10.1016/j.carbpol.2017.07.028
  • Peer Reviewed
• [Journal Article] Pharmacokinetics of lipophilically different 3-substituted 2,2,5,5-tetramethylpyrrolidine-N-oxyl radicals frequently used as redox probes in in vivo magnetic resonance studies (2016)
  • Author(s): Keizo Takeshita, Shoko Okazaki, Yuriko Hirose
  • Journal Title: Free Radical Biology and Medicine Volume: 97 Pages: 263-273
  • DOI: 10.1016/j.freeradbiomed.2016.06.008
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 敗血症モデルマウスにおける生体内レドックスの経時的変化と病態指標 (2016)
  • Author(s): 岡崎祥子、北富章子、佐藤みづき、福田偲、竹下啓蔵
  • Organizer: 第69回酸化ストレス学会学術集会
  • Place of Presentation: 仙台国際センター（宮城県・仙台市）
  • Year and Date: 2016-08-30
• [Presentation] 敗血症モデルマウスにおける生体内レドックスの非侵襲的測定と病態変化 (2015)
  • Author(s): 岡崎祥子、佐藤みづき、福田 偲、北富章子、竹下啓蔵
  • Organizer: 第32回日本薬学会九州支部大会
  • Place of Presentation: 九州保健福祉大（宮崎県・延岡市）
  • Year and Date: 2015-11-28
• [Presentation] 生体内レドックス測定に用いられる五員環ニトロキシルスピンプローブの薬物動態解析 (2015)
  • Author(s): 岡崎祥子、廣瀬友吏子、竹下啓蔵
  • Organizer: 第54回電子スピンサイエンス学会
  • Place of Presentation: 朱鷺メッセ（新潟県・新潟市）
  • Year and Date: 2015-11-02