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Study of translational regulation mediated gene expression relating cell proliferation and differentiation by polyamines

Research Project

Project/Area Number 15K07921
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biological pharmacy
Research InstitutionChiba University

Principal Investigator

NISHIMURA Kazuhiro  千葉大学, 大学院薬学研究院, 准教授 (60302569)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsポリアミン / 翻訳 / RNA / ショ糖密度勾配遠心 / メタボローム / ヌクレオチド代謝 / KLF4 / ポリソーム / シャペロニン / 翻訳制御 / ポリソームフラクション / シャペロニン蛋白質 / Kruppel-like factor 4 / ヒスタミン
Outline of Final Research Achievements

To clarify genes whose are translationally regulated by polyamines essential for cell proliferation and differentiation, we performed to identify proteins that change depending on the presence or absence of intracellular polyamines by using some kinds of method. As a result, it was strongly suggested that five proteins are translationally regulated by polyamines, because the amount of these proteins are decreased due to the reduction of intracellular polyamines. In addition, as a result of examining the mechanism of translational stimulation of transcription factor Klf4 by polyamines, we clarified that the 5'-untranslated region (5'-UTR) of Klf4 mRNA was important for translational regulation by polyamines.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (24 results)

All 2018 2017 2016 2015 Other

All Journal Article (6 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 5 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (17 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] Design, synthesis, and evaluation of polyamine-memantine hybrids as NMDA channel blockers.2018

    • Author(s)
      Kumamoto T, Nakajima M, Uga R, Ihayazaka N, Kashihara H, Katakawa K, Ishikawa T, Saiki R, Nishimura K, Igarashi K.
    • Journal Title

      Bioorg Med Chem

      Volume: 26 Issue: 3 Pages: 603-608

    • DOI

      10.1016/j.bmc.2017.12.021

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Cloning and Expression of Recombinant Chondroitinase ACII and Its Comparison to the Arthrobacter aurescens Enzyme.2017

    • Author(s)
      Williams A, He W, Cress BF, Liu X, Alexandria J, Yoshizawa H, Nishimura K, Toida T, Koffas M, Linhardt RJ.
    • Journal Title

      Biotechnol. J.

      Volume: 12 Issue: 10 Pages: 201700239-201700239

    • DOI

      10.1002/biot.201700239

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Polyamines release the let-7b-mediated suppression of initiation codon recognition during the protein synthesis of EXT2.2016

    • Author(s)
      Imamura M, Higashi K, Yamaguchi K, Asakura K, Furihata T, Terui Y, Satake T, Maegawa J, Yasumura K, Ibuki A, Akase T, Nishimura K, Kashiwagi K, Linhardt RJ, Igarashi K, Toida T
    • Journal Title

      Sci. Rep.

      Volume: 6 Issue: 1 Pages: 33549-33549

    • DOI

      10.1038/srep33549

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] eIF5Aとハイプシンの今昔物語2016

    • Author(s)
      西村 和洋
    • Journal Title

      ポリアミン

      Volume: 3 Pages: 2-7

    • Related Report
      2016 Research-status Report
    • Open Access
  • [Journal Article] Toxic acrolein production due to Ca(2+) influx by the NMDA receptor during stroke.2016

    • Author(s)
      Nakamura M, Uemura T, Saiki R, Sakamoto A, Park H, Nishimura K, Terui Y, Toida T, Kashiwagi K, Igarashi K.
    • Journal Title

      Atherosclerosis

      Volume: 244 Pages: 131-137

    • DOI

      10.1016/j.atherosclerosis.2015.11.012

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Aggravation of brain infarction through an increase in acrolein production and a decrease in glutathione with aging.2016

    • Author(s)
      Uemura T, Watanabe K, Ishibashi M, Saiki R, Kuni K, Nishimura K, Toida T, Kashiwagi K, Igarashi K.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 473 Issue: 2 Pages: 630-635

    • DOI

      10.1016/j.bbrc.2016.03.137

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] ポリアミンによるコンドロイチン4-O-硫酸基転移酵素2 の合成促進機構の解明2018

    • Author(s)
      東恭平、朝倉希里生、今村正隆、工藤遥香、山口勝利、西村和洋、五十嵐一衛、戸井田敏彦
    • Organizer
      日本ポリアミン学会第9回年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ポリアミンにより翻訳促進を受ける蛋白質の同定2018

    • Author(s)
      吉野哲彦、吉澤祐希、戸井田敏彦、五十嵐一衛、西村和洋
    • Organizer
      日本薬学会第138年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ポリアミンによりコンドロイチン硫酸合成調節機構の解明2018

    • Author(s)
      朝倉希里生、東恭平、今村正隆、山口勝利、降幡知巳、西村和洋、五十嵐一衛、戸井田敏彦
    • Organizer
      日本薬学会第138年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] アプタマー取得に向けた樹脂担持型ハイプシンの設計と合成2018

    • Author(s)
      太田礼伊也、小黒明広、西村和洋、藤岡弘道、有澤光弘
    • Organizer
      日本薬学会第138年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 加齢による脳梗塞悪化とアクロレインの関係並びに新規脳保護薬の開発2017

    • Author(s)
      渡辺健太、植村武史、東恭平、小暮紀行、北島満里子、高山廣光、高尾浩一、杉田義昭、西村和洋、五十嵐一衛、戸井田敏彦
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2017-03-24
    • Related Report
      2016 Research-status Report
  • [Presentation] ポリアミンによるChondroitin synthase-1合成促進機構の解明2017

    • Author(s)
      東恭平、山口勝利、今村正隆、朝倉希里生、西村和洋、五十嵐一衛、戸井田敏彦
    • Organizer
      日本ポリアミン学会第8回年会
    • Place of Presentation
      千葉工業大学(千葉県習志野市)
    • Year and Date
      2017-01-20
    • Related Report
      2016 Research-status Report
  • [Presentation] ポリアミン代謝阻害剤によるメタボローム解析2017

    • Author(s)
      吉澤祐希、吉野哲彦、戸井田敏彦、五十嵐一衛、西村和洋
    • Organizer
      「ポリアミンと核酸の共進化」第15回合同シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] ポリアミンによるKLF4の翻訳制御は骨髄由来マスト細胞分化のヒスタミン産生を抑制する2017

    • Author(s)
      西村和洋、岡本萌実、澁江梨奈、水田俊男、芝山徹、田中智之、東恭平、戸井田敏彦、五十嵐一衛
    • Organizer
      ConBio2017(第40回日本分子生物学会年会・第90回日本生化学会大会合同大会)
    • Related Report
      2017 Annual Research Report
  • [Presentation] ポリアミンによるヌクレオチド代謝への影響2017

    • Author(s)
      吉澤祐希、吉野哲彦、戸井田敏彦、五十嵐一衛、西村和洋
    • Organizer
      ConBio2017(第40回日本分子生物学会年会・第90回日本生化学会大会合同大会)
    • Related Report
      2017 Annual Research Report
  • [Presentation] シャペロニンCCTの機能に対するポリアミンの影響2016

    • Author(s)
      渡邊紗貴、小髙愛彩、戸井田敏彦、五十嵐一衛、西村和洋
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
  • [Presentation] ポリアミンによるコンドロイチン硫酸合成調節機構の解明2016

    • Author(s)
      朝倉希里生、東恭平、今村正隆、山口勝利、降幡知巳、西村和洋、五十嵐一衛、戸井田敏彦
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
  • [Presentation] ポリアミンによるコンドロイチン硫酸合成調節機構の解明2016

    • Author(s)
      朝倉希里生、東恭平、今村正隆、山口勝利、西村和洋、五十嵐一衛、戸井田敏彦
    • Organizer
      「ポリアミンと核酸の共進化」第14回合同シンポジウム
    • Place of Presentation
      東京慈恵会医科大学(東京都港区)
    • Year and Date
      2016-06-18
    • Related Report
      2016 Research-status Report
  • [Presentation] 加齢による脳梗塞リスクの上昇には、ポリアミン代謝の活性化とグルタチオン合成の低下が関わる2016

    • Author(s)
      植村武史、渡辺健太、石橋美咲、斎木遼太郎、西村和洋、戸井田敏彦、五十嵐一衛
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-03-26
    • Related Report
      2015 Research-status Report
  • [Presentation] マスト細胞におけるポリアミンのヒスタミン生合成抑制機構の解明2015

    • Author(s)
      水田俊男、西村和洋、芝山徹、田中智之、戸井田敏彦、五十嵐一衛
    • Organizer
      BMB2015(第38回日本分子生物学会年会・第88回日本生化学会大会合同大会)
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 脳梗塞時のポリアミン代謝における加齢の影響2015

    • Author(s)
      渡辺健太、石橋美咲、斎木遼太郎、植村武史、西村和洋、戸井田敏彦、五十嵐一衛
    • Organizer
      日本ポリアミン学会第7回年会
    • Place of Presentation
      京都工芸繊維大学
    • Year and Date
      2015-11-13
    • Related Report
      2015 Research-status Report
  • [Presentation] ポリアミンの生理作用とバイオマーカーへの展開2015

    • Author(s)
      西村和洋
    • Organizer
      第9回次世代を担う若手医療薬科学シンポジウム
    • Place of Presentation
      千葉大学薬学部120周年記念講堂
    • Year and Date
      2015-11-07
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Histamine synthesis is regulated by polyamines in mouse bone marrow derived mast cells2015

    • Author(s)
      Nishimura, K., Mizuta, T., Shibayama, T., Tanaka, S., Toida, T., and Igarashi, K.
    • Organizer
      Gordon Research Conference on Polyamines
    • Place of Presentation
      Waterville Valley, NH, USA
    • Year and Date
      2015-06-14
    • Related Report
      2015 Research-status Report
  • [Remarks] 千葉大学大学院薬学研究院病態分析化学研究室

    • URL

      http://www.p.chiba-u.jp/lab/bunseki/index.html

    • Related Report
      2016 Research-status Report 2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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