Study of translational regulation mediated gene expression relating cell proliferation and differentiation by polyamines
Project/Area Number |
15K07921
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biological pharmacy
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Research Institution | Chiba University |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | ポリアミン / 翻訳 / RNA / ショ糖密度勾配遠心 / メタボローム / ヌクレオチド代謝 / KLF4 / ポリソーム / シャペロニン / 翻訳制御 / ポリソームフラクション / シャペロニン蛋白質 / Kruppel-like factor 4 / ヒスタミン |
Outline of Final Research Achievements |
To clarify genes whose are translationally regulated by polyamines essential for cell proliferation and differentiation, we performed to identify proteins that change depending on the presence or absence of intracellular polyamines by using some kinds of method. As a result, it was strongly suggested that five proteins are translationally regulated by polyamines, because the amount of these proteins are decreased due to the reduction of intracellular polyamines. In addition, as a result of examining the mechanism of translational stimulation of transcription factor Klf4 by polyamines, we clarified that the 5'-untranslated region (5'-UTR) of Klf4 mRNA was important for translational regulation by polyamines.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Design, synthesis, and evaluation of polyamine-memantine hybrids as NMDA channel blockers.2018
Author(s)
Kumamoto T, Nakajima M, Uga R, Ihayazaka N, Kashihara H, Katakawa K, Ishikawa T, Saiki R, Nishimura K, Igarashi K.
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Journal Title
Bioorg Med Chem
Volume: 26
Issue: 3
Pages: 603-608
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Polyamines release the let-7b-mediated suppression of initiation codon recognition during the protein synthesis of EXT2.2016
Author(s)
Imamura M, Higashi K, Yamaguchi K, Asakura K, Furihata T, Terui Y, Satake T, Maegawa J, Yasumura K, Ibuki A, Akase T, Nishimura K, Kashiwagi K, Linhardt RJ, Igarashi K, Toida T
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Journal Title
Sci. Rep.
Volume: 6
Issue: 1
Pages: 33549-33549
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Histamine synthesis is regulated by polyamines in mouse bone marrow derived mast cells2015
Author(s)
Nishimura, K., Mizuta, T., Shibayama, T., Tanaka, S., Toida, T., and Igarashi, K.
Organizer
Gordon Research Conference on Polyamines
Place of Presentation
Waterville Valley, NH, USA
Year and Date
2015-06-14
Related Report
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