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Development of next generation of baculovirus vectors with tissue specific gene transduction

Research Project

Project/Area Number 15K07926
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biological pharmacy
Research InstitutionKanazawa University

Principal Investigator

Tamura Takahiko  金沢大学, 薬学系, 助教 (00434035)

Co-Investigator(Renkei-kenkyūsha) SAKAGUCHI Miako  長崎大学, 熱帯医学研究所, 助教 (50400651)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsバキュロウイルス / マラリア原虫 / バキュロウイルスベクター / マラリア
Outline of Final Research Achievements

In this research, budded recombinant baculovirus vectors (BVs) have been developed because BVs have many advantages for tissue targeting gene delivery vectors.It is easily possible to display exogenous proteins on the surface of BV virion. CSP and TRAP, malaria sporozoite surface proteins have highly selective affinity to hepatocytes. BVs expressing CSP or TRAP molecule on the virion surface have been constructed and they were shown to greatly increase transduction efficacy to human hepatoma cell line (HepG2 et al.) and human primary hepatocytes (PXB cells). Furthermore, to overcome the vulnerability to serum complement system, fusion molecules of complement regulatory proteins were displayed to BV virion surfaces. These BVs were shown to greatly increase resistance against complement attack.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2017 2016 2015 Other

All Int'l Joint Research (2 results) Journal Article (2 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Int'l Joint Research] INSERM(フランス)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] INSERM(フランス)

    • Related Report
      2016 Research-status Report
  • [Journal Article] 組織標的性を持つバキュロウイルスの開発2017

    • Author(s)
      田村隆彦
    • Journal Title

      月刊「細胞」

      Volume: 49(4) Pages: 44-46

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Malaria sporozoite protein expression enhances baculovirus-mediated gene transfer to hepatocytes.2016

    • Author(s)
      Tamura T, Kawabata C, Matsushita S, Sakaguchi M, Yoshida S
    • Journal Title

      Journal of Gene Medicine

      Volume: 印刷中 Issue: 4-6 Pages: 75-85

    • DOI

      10.1002/jgm.2879

    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 補体抵抗性を持った肝細胞標的性バキュロウイルスベクターの開発2016

    • Author(s)
      田村隆彦、川端千明、川井悠輔、坂口美亜子、吉田栄人
    • Organizer
      日本分子生物学分子生物学学会
    • Place of Presentation
      横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] 組織標的性を持つ遺伝子導入ベクターとしての次世代バキュロウイルスの開発2015

    • Author(s)
      田村隆彦、川井悠輔、川端千明、松下俊介、坂口美亜子、吉田栄人
    • Organizer
      日本分子生物学学会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-04
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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