Inhibition of S1P receptor signal transduction by extracellular alpha-synuclein
Project/Area Number |
15K07930
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biological pharmacy
|
Research Institution | Kobe University |
Principal Investigator |
OKADA TARO 神戸大学, 医学研究科, 准教授 (80304088)
|
Project Period (FY) |
2015-10-21 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | α-シヌクレイン / S1P受容体 / パーキンソン病 / S1P / スフィンゴシン1-リン酸 / スフィンゴ脂質 |
Outline of Final Research Achievements |
Alpha-synuclein (ASN) is considered to be involved in the pathogenesis of Parkinson's disease. ASN is know to be localized outside of neurons but its physiological and/or pathological relevance has not known. In this study I showed extracellular ASN inhibits S1P1 receptor-G protein coupling for the first time. Interestingly the effect of ASN was specific to S1P1 receptor and S1P2 receptor-G protein coupling was not inhibited by extracellular ASN at all. Majority of S1P1 receptor is localized in raft fraction in the cells and extracellular ASN treatment cause "exit" of S1P1 receptor from the raft fraction. These results are important because specific action of extracellular ASN has not been known and we already reported that S1P1 receptor plays important role in hippocampal neurons.
|
Report
(4 results)
Research Products
(9 results)
-
-
[Journal Article] Involvement of Gβγ subunits of Gi protein coupled with S1P receptor on multivesicular endosomes in F-actin formation and cargo sorting into exosomes2018
Author(s)
Kajimoto, T., Mohamed, N.N.I., Badawy, S.M.M., Matovelo, S.A., Hirase, M., Nakamura, S., Yoshida, D., Okada, T., Ijuin, T., Nakamura, S.
-
Journal Title
Journal of Biological Chemistry
Volume: 293
Issue: 1
Pages: 245-253
DOI
NAID
Related Report
Peer Reviewed
-
-
-
[Journal Article] Impairment of PDGF-induced chemotaxis by extracellular α-synuclein through selective inhibition of Rac1 activation2016
Author(s)
Okada, T., Hirai, C., Badawy, S., Zhang, L., Kajimoto, T., Nakamura, SI.
-
Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 37810-37810
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
-
-