Molecular mechanism of ciliary vesicle formation in mammalian cells

• Project/Area Number: 15K07931
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Kobayashi Tetsuo 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (80433994)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 一次繊毛 / 繊毛小胞

Outline of Final Research Achievements

By comprehensive proteome screen, we identified novel small GTPase Rab-like 3 (Rabl3) as a putative binding partner of Rab11 which is involved in ciliary vesicle formation. We showed that Rabl3 physically interacts with Rab11. We also found that Rabl3 localizes to centrioles by immunofluorescence analysis in RPE1 cells. Furthermore, we observed that Rabl3 depletion causes decrease of primary cilia formation in RPE1 cells.

Research Products

• [Journal Article] 一次繊毛構築のはじまりの制御機構 (2018)
  • Author(s): 小林 哲夫
  • Journal Title: 実験医学 Volume: 36 Pages: 921-925
• [Journal Article] HDAC2 promotes loss of primary cilia in pancreatic ductal adenocarcinoma (2017)
  • Author(s): Kobayashi T, Nakazono K, Tokuda M, Mashima Y, Dynlacht BD, Itoh H
  • Journal Title: EMBO Reports Volume: 18 Issue: 2 Pages: 334-343
  • DOI: 10.15252/embr.201541922
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Loss of a primary cilium in PDAC (2017)
  • Author(s): Kobayashi T, Itoh H
  • Journal Title: Cell Cycle Volume: - Issue: 9 Pages: 1-2
  • DOI: 10.1080/15384101.2017.1304738
  • Open Access
• [Presentation] Molecular mechanism of loss of primary cilia in pancreatic ductal adenocarcinoma cells (2018)
  • Author(s): Tetsuo Kobayashi, Yu Mashima, Ayano Shoda, Brian David Dynlacht, Hiroshi Itoh
  • Organizer: Cold Spring Harbor Asia Conference “Cilia and Centrosomes”
  • Int'l Joint Research
• [Presentation] 膵管癌における一次繊毛消失機構の解析 (2018)
  • Author(s): 小林 哲夫、馬島 友、庄田 彩乃、Brian David Dynlacht、伊東 広
  • Organizer: 第69回日本細胞生物学会
• [Presentation] 膵管癌細胞における一次繊毛消失メカニズムの解析 (2018)
  • Author(s): 馬島 友、小林 哲夫、伊東 広
  • Organizer: 2017年度生命科学系学会合同年次大会
• [Presentation] HDAC2 promotes loss of primary cilia in pancreatic ductal adenocarcinoma (2016)
  • Author(s): Kobayashi T, Nakazono K, Tokuda M, Mashima Y, Dynlacht BD, Itoh H
  • Organizer: The 28th CDB meeting
  • Place of Presentation: 理研CDB、神戸市、兵庫県
  • Year and Date: 2016-11-28
  • Int'l Joint Research
• [Presentation] 膵管癌細胞における一次繊毛消失メカニズムの解析 (2015)
  • Author(s): 小林 哲夫、中薗 昂亮、徳田 澪、馬島 友、Brian David Dynlacht、伊東 広
  • Organizer: 第38回日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド、神戸市、兵庫県
  • Year and Date: 2015-12-01
• [Presentation] 膵管癌細胞における一次繊毛消失機構の解析 (2015)
  • Author(s): 小林 哲夫、中薗 昂亮、徳田 澪、馬島 友、伊達山 泉、Brian David Dynlacht、伊東 広
  • Organizer: 第6回繊毛研究会
  • Place of Presentation: 基礎生物学研究所、岡崎市、愛知県
  • Year and Date: 2015-11-13
• [Presentation] Analysis of serotonin signaling mediated through primary cilia (2015)
  • Author(s): Dateyama Izumi, Tetsuo Kobayashi, Hiroshi Itoh
  • Organizer: The 4th International Conference on Pharmacy and Advanced Pharmaceutical Sciences
  • Place of Presentation: Yogyakarta, Indonesia
  • Year and Date: 2015-09-07
  • Int'l Joint Research
• [Remarks] 奈良先端科学技術大学院大学先端科学技術研究科バイオサイエンス領域分子情報薬理学研究室ホームページ
  • URL: http://bsw3.naist.jp/itoh/
• [Remarks] 奈良先端科学技術大学院大学バイオサイエンス研究科分子情報薬理学研究室ホームページ
  • URL: http://bsw3.naist.jp/itoh/