Mechanism of sugar chain elongation catalyzed by muscular dystrophy cause gene product LARGE

• Project/Area Number: 15K07935
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Nagoya City University
• Principal Investigator: Yagi Hirokazu 名古屋市立大学, 大学院薬学研究科, 講師 (70565423)
• Co-Investigator(Renkei-kenkyūsha): KATO Koichi 名古屋市立大学, 大学院薬学研究科, 教授 (20211849) ; SUGIYAMA Masaaki 京都大学, 複合原子力科学研究所, 教授 (10253395) ; SATOH Tadashi 名古屋市立大学, 大学院薬学研究科, 准教授 (80532100) ; YAMAGUCHI Takumi 北陸先端科学技術大学院大学, マテリアルサイエンス系, 准教授 (60522430)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: LARGE / K4CP / 糖転移酵素 / 高速AFM / 糖鎖伸長 / バイファンクショナルな糖転移酵素 / Ｘ線小角散乱 / 非変性質量分析 / 筋ジストロフィー

Outline of Final Research Achievements

LARGE (Like-acetylglucosaminyltransferase) is the causative gene product for congenital muscular dystrophy which possesses two catalytic domains with homology to members of glycosyltransferases to form a repeat sequence of xylose and glucuronic acid residues. In this study, we have performed a molecular dynamics study to characterize the mechanism that the enzymes composed of these two catalytic domains catalyze the elongation reactions of sugar chains. As a result, the enzymes exhibited dynamic motions involving domain-domain association and dissociation during the reaction, indicating that orientation between the two domains would contribute to the elongation efficiency to exchange the substrate sugar chain.

Research Products

• [Int'l Joint Research] Academia Sinica(台湾)
• [Int'l Joint Research] UMRS1138, University; Paris Descartes,/Institut Laue-Langevin(フランス)
• [Int'l Joint Research] Academia Sinica(Taiwan)
• [Int'l Joint Research] Albert Einstein College of Medicine(U.S.A.)
• [Journal Article] Site-specific N-glycosylation analysis of soluble Fcγ receptor IIIb in human serum (2018)
  • Author(s): Yagi Hirokazu、Takakura Daisuke、Roumenina Lubka T.、Fridman Wolf Herman、Sautes-Fridman Catherine、Kawasaki Nana、Kato Koichi
  • Journal Title: Sci. Rep. Volume: 8 Issue: 1 Pages: 2719-2719
  • DOI: 10.1038/s41598-018-21145-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Stable isotope labeling approaches for NMR characterization of glycoproteins using eukaryotic expression systems (2018)
  • Author(s): Yanaka Saeko、Yagi Hirokazu、Yogo Rina、Yagi-Utsumi Maho、Kato Koichi
  • Journal Title: Journal of Biomolecular NMR Volume: - Issue: 3 Pages: 1-10
  • DOI: 10.1007/s10858-018-0169-2
  • Peer Reviewed / Open Access
• [Journal Article] 3D structural analysis of protein O-mannosyl kinase, POMK, a causative gene product of dystroglycanopathy (2017)
  • Author(s): M.Nagae, S.K.Mishra, M.Neyazaki, R.Oi, A.Ikeda, N.Matsugaki, S.Akashi, H.Manya, M.Mizuno, H.Yagi, K.Kato, T.Senda, T.Endo, T.Nogi, and Y.Yamaguchi
  • Journal Title: Genes Cells Volume: - Issue: 4 Pages: 348-359
  • DOI: 10.1111/gtc.12480
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Alteration of a recombinant protein N-glycan structure in silkworms by partial suppression of N-acetylglucosaminidase gene expression (2017)
  • Author(s): Kato Tatsuya、Kikuta Kotaro、Kanematsu Ayumi、Kondo Sachiko、Yagi Hirokazu、Kato Koichi、Park Enoch Y.
  • Journal Title: Biotechnol. Lett. Volume: 39 Issue: 9 Pages: 1299-1308
  • DOI: 10.1007/s10529-017-2361-y
  • NAID: 120006469813
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] NMR Detection of Semi-Specific Antibody Interactions in Serum Environments (2017)
  • Author(s): Yanaka Saeko、Yamazaki Toshio、Yogo Rina、Noda Masanori、Uchiyama Susumu、Yagi Hirokazu、Kato Koichi
  • Journal Title: Molecules Volume: 22 Issue: 10 Pages: 1619-1619
  • DOI: 10.3390/molecules22101619
  • Peer Reviewed / Open Access
• [Journal Article] Technical Basis for Nuclear Magnetic Resonance Approach for Glycoproteins (2017)
  • Author(s): Kato Koichi、Yanaka Saeko、Yagi Hirokazu
  • Journal Title: Experimental Approaches of NMR Spectroscopy Volume: 1 Pages: 415-438
  • DOI: 10.1007/978-981-10-5966-7_15
  • ISBN: 9789811059650, 9789811059667
  • Peer Reviewed
• [Journal Article] Characterization of conformational deformation-coupled interaction between immunoglobulin G1 Fc glycoprotein and a low-affinity Fcγ receptor by deuteration-assisted small-angle neutron scattering (2017)
  • Author(s): Yogo Rina、Yanaka Saeko、Yagi Hirokazu、Martel Anne、Porcar Linoel、Ueki Yutaro、Inoue Rintaro、Sato Nobuhiro、Sugiyama Masaaki、Kato Koichi
  • Journal Title: Biochemistry and Biophysics Reports Volume: 12 Pages: 1-4
  • DOI: 10.1016/j.bbrep.2017.08.004
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Conformational effects of N-glycan core fucosylation of immunoglobulin G Fc region on its interaction with Fcγ receptor IIIa (2017)
  • Author(s): Sakae Yoshitake、Satoh Tadashi、Yagi Hirokazu、Yanaka Saeko、Yamaguchi Takumi、Isoda Yuya、Iida Shigeru、Okamoto Yuko、Kato Koichi
  • Journal Title: Sci. Rep. Volume: 7 Issue: 1 Pages: 13780-13780
  • DOI: 10.1038/s41598-017-13845-8
  • NAID: 120006545940
  • Peer Reviewed / Open Access
• [Journal Article] Lewis X-Carrying Neoglycolipids Evoke Selective Apoptosis in Neural Stem Cells (2017)
  • Author(s): Yagi Hirokazu、Yan Gengwei、Suzuki Tatsuya、Tsuge Shingo、Yamaguchi Takumi、Kato Koichi
  • Journal Title: Neurochemical Research Volume: 43 Issue: 1 Pages: 212-218
  • DOI: 10.1007/s11064-017-2415-5
  • Peer Reviewed
• [Journal Article] GlcNAc6ST-1 regulates sulfation of N-glycans and myelination in the peripheral nervous system. (2017)
  • Author(s): Yoshimura T, Hayashi A, Handa-Narumi M, Yagi H, Ohno N, Koike T, Yamaguchi Y, Uchimura K, Kadomatsu K, Sedzik J, Kitamura K, Kato K, Trapp BD, Baba H, Ikenaka K.
  • Journal Title: Sci Rep. Volume: 7 Issue: 1 Pages: 42257-42257
  • DOI: 10.1038/srep42257
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Direct mapping of additional modifications on phosphorylated O-glycans of α-dystroglycan by mass spectrometry analysis in conjunction with knocking out of causative genes for dystroglycanopathy (2016)
  • Author(s): H.Yagi, C.-W.Kuo, T.Obayashi, S.Ninagawa, K.-H.Khoo, and K.Kato
  • Journal Title: Mol. Cell Proteomics Volume: 15 Issue: 11 Pages: 3424-3434
  • DOI: 10.1074/mcp.m116.062729
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Functional roles of glycoconjugates in the maintenance of stemness and differentiation process of neural stem cells (2016)
  • Author(s): Hirokazu Yagi and Koichi Kato
  • Journal Title: Glycoconjugate J. Volume: ‐ Issue: 6 Pages: 1-7
  • DOI: 10.1007/s10719-016-9707-x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Comparison of analytical methods for profiling N- and O-linked glycans from cultured cell lines : HUPO Human Disease Glycomics/Proteome Initiative multi-institutional study (2016)
  • Author(s): Hiromi Ito et al.
  • Journal Title: Glycoconjugate J. Volume: 未定 Issue: 3 Pages: 405-415
  • DOI: 10.1007/s10719-015-9625-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Stable isotope labeling of glycoprotein expressed in silkworms using immunoglobulin G as a test molecule (2015)
  • Author(s): H.Yagi, M.Nakamura, J.Yokoyama, Y.Zhang, T.Yamaguchi, S.Kondo, J.Kobayashi, T.Kato, E.Y.Park, S.Nakazawa, N.Hashii, N.Kawasaki, and K.Kato
  • Journal Title: J. Biomol. NMR Volume: - Issue: 2 Pages: 157-167
  • DOI: 10.1007/s10858-015-9930-y
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Glycan structure and serum half-life of recombinant CTLA4Ig, an immunosuppressive agent, expressed in suspension-cultured rice cells with coexpression of human β1,4-galactosyltransferase and human CTLA4Ig (2015)
  • Author(s): S.H.Kang, H.S.Jung, S.J.Lee1, C.I.Park, S.M.Lim, H.Park, B.S.Kim, K.H.Na, G.J.Han, J.W.Bae, H.J.Park, K.C.Bang, B.T.Park, H.S.Hwang, I.-Soo .Jung, J.I.Kim, D.B.Oh, D.I.Kim, H.Yagi, K.Kato, D.K.Kim, and H.H.Kim
  • Journal Title: Glycoconjugate J. Volume: - Issue: 3-4 Pages: 161-172
  • DOI: 10.1007/s10719-015-9590-x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Importance of the side chain at position 296 of antibody Fc in interactions with FcγRIIIa and other Fcγ receptors (2015)
  • Author(s): Yuya Isoda, Hirokazu Yagi, Tadashi Satoh, Mami Shibata-Koyama, Kazuhiro Masuda, Mitsuo Satoh,Koichi Kato, and Shigeru Iida
  • Journal Title: PLoS ONE Volume: 10 Issue: 10 Pages: e0140120-e0140120
  • DOI: 10.1371/journal.pone.0140120
  • Peer Reviewed / Open Access
• [Journal Article] Ectopic clustering of Cajal-Retzius and subplate cells is an initial pathological feature in Pomgnt2-knockout mice, a model of dystroglycanopathy (2015)
  • Author(s): Naoki Nakagawa, Hirokazu Yagi, Koichi Kato, Hiromu Takematsu, and Shogo Oka
  • Journal Title: Sci. Rep. Volume: 5 Issue: 1 Pages: 11163-11163
  • DOI: 10.1038/srep11163
  • NAID: 120005670888
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] α-ジストログリカンのラミニン結合性糖鎖形成するポストリン酸修飾の構造解析 (2017)
  • Author(s): 矢木宏和，Chu-Wei KUO，尾林卓幸，蜷川 暁，Kay-Hooi KHOO，加藤晃一
  • Organizer: 日本薬学会 第137年会
  • Place of Presentation: 東北大学川内北キャンパス（宮城県仙台市）
  • Year and Date: 2017-03-25
• [Presentation] α-ジストログリカンの上のポストリン酸修飾の構造解析 (2017)
  • Author(s): 矢木宏和
  • Organizer: 平成28年度 新学術領域「神経糖鎖生物学」最終シンポジウム
  • Place of Presentation: JPタワー名古屋 ホール＆カンファレンス（愛知県名古屋市）
  • Year and Date: 2017-03-03
• [Presentation] Development and application of glycosylation-profiling techniques for functional glycomics in the nervous system (2017)
  • Author(s): Hirokazu Yagi
  • Organizer: 11th International Symposium on Cell Surface Macromolecules
  • Place of Presentation: Indian Institute of Science Education and Research (IISER) Mohali（Chandegarh, India）
  • Year and Date: 2017-02-26
  • Int'l Joint Research / Invited
• [Presentation] タンパク質における糖鎖修飾の生物学的機能と高次構造への影響 (2017)
  • Author(s): 矢木宏和
  • Organizer: 第1回名古屋市立大学若手イブニングセミナー
  • Invited
• [Presentation] 糖タンパク質における糖鎖の生物機能と高次構造への影響 (2017)
  • Author(s): 矢木宏和
  • Organizer: 第58回 日本生化学会中国四国支部例会
  • Invited
• [Presentation] FUT9によるキャリアタンパク質特異的なフコース修飾機構の解明 (2017)
  • Author(s): 齋藤泰輝，矢木宏和，柘植信吾，Chuwei Kuo，Kay-Hooi Khoo，加藤晃一
  • Organizer: 第81回 日本生化学会中部支部例会・シンポジウム
• [Presentation] フコース転移酵素によるキャリアタンパク質特異的なLewisX修飾機構 (2017)
  • Author(s): 矢木宏和，齋藤泰輝，姫田美咲，柘植信吾，加藤晃一
  • Organizer: 第69回日本細胞生物学会大会
• [Presentation] 糖タンパク質糖鎖の機能解明のための構造解析技術の開発と応用 (2017)
  • Author(s): 矢木宏和
  • Organizer: 第1回触発型有機化学研究会
  • Invited
• [Presentation] The characterization of the laminin-binding glycans on alpha-dystroglycan catalyzed by several causative gene products of dystroglycanopathy (2017)
  • Author(s): Hirokazu Yagi
  • Organizer: The 1st International Conference on the Glycobiology of Nervous System
  • Int'l Joint Research / Invited
• [Presentation] Mechanisms underlying the carrier protein-specific lewis X modification catalyzed by fucosyltransferase IX (2017)
  • Author(s): Taiki Saito, Hirokazu Yagi, Shingo Tsuge, Chuwei Kuo, Kay-Hooi Khoo, and Koichi Kato
  • Organizer: The 1st International Conference on the Glycobiology of Nervous System
  • Int'l Joint Research
• [Presentation] 糖タンパク質糖鎖の構造解析法の開発と糖鎖機能解析への応用 (2017)
  • Author(s): 矢木宏和
  • Organizer: 第2回G-CHAINセミナー
  • Invited
• [Presentation] 糖タンパク質糖鎖の機能解明のための構造生物学的アプローチ法の開発と応用 (2017)
  • Author(s): 矢木宏和
  • Organizer: 第26回 日本バイオイメージング学会学術集会
  • Invited
• [Presentation] α-ジストログリカンに発現するホスホジエステル結合を有する糖鎖の構造機能解析 (2017)
  • Author(s): 尾林卓幸，矢木宏和，Chu-Wei Kuo，蜷川 暁，Kay-Hooi Khoo，加藤晃一
  • Organizer: 第14回「若手の力フォーラム」
• [Presentation] フコース転移酵素9によるキャリアタンパク質特異的なフコース修飾機構の解明 (2017)
  • Author(s): 齋藤泰輝，矢木宏和，Chu-Wei Kuo，Kay-Hooi Khoo，加藤晃一
  • Organizer: 第14回「若手の力フォーラム」
• [Presentation] フコース転移酵素のキャリアタンパク質に対する特異的なLewisX修飾機構 (2017)
  • Author(s): 矢木宏和，齋藤泰輝，姫田美咲，柘植信吾，加藤晃一
  • Organizer: 2017年度 生命科学系学会合同年次大会（ConBio2017）
• [Presentation] キャリアータンパク質特異的なLewisX糖鎖修飾機構の解析 (2016)
  • Author(s): 柘植信吾，姫田美咲，矢木宏和，加藤晃一
  • Organizer: 糖鎖科学中部拠点 第13回「若手の力」フォーラム
  • Place of Presentation: 岐阜大学（岐阜県岐阜市）
  • Year and Date: 2016-11-26
• [Presentation] ２つの異なる転移活性ドメインを有する糖転移酵素による糖鎖伸長機構の解析 (2016)
  • Author(s): 金 明美，吉田早希，小財稔矢，内橋貴之，杉山正明，矢木宏和，加藤晃一
  • Organizer: 糖鎖科学中部拠点 第13回「若手の力」フォーラム
  • Place of Presentation: 岐阜大学（岐阜県岐阜市）
  • Year and Date: 2016-11-26
• [Presentation] 細胞膜上の糖鎖の機能解明のための糖鎖クラスターの設計と創生 (2016)
  • Author(s): 矢木宏和
  • Organizer: 第38回 生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 名古屋市立大学薬学部 宮田専治記念ホール（愛知県名古屋市）
  • Year and Date: 2016-11-17
• [Presentation] キャリア依存的なLewisX糖鎖修飾を担う特異的なゴルジ体の局在 (2016)
  • Author(s): 姫田美咲，柘植信吾，矢木宏和，加藤晃一
  • Organizer: 第25回 日本バイオイメージング学会学術集会
  • Place of Presentation: 名古屋市立大学薬学部 宮田専治記念ホール（愛知県名古屋市）
  • Year and Date: 2016-09-05
• [Presentation] ヒト血清由来の可溶型FcγレセプターIIIbの部位特異的なN型糖鎖の構造解析 (2016)
  • Author(s): 矢木宏和，高倉大輔，Lubka T. Roumenina，Wolf Herman Fridman，Catherine Sautes-Fridman，川崎ナナ，加藤晃一
  • Organizer: 第35回 日本糖質学会年会
  • Place of Presentation: 高知市文化プラザ かるぽーと（高知県高知市）
  • Year and Date: 2016-09-03
• [Presentation] 分子、細胞、組織レベルにおける糖鎖の機能解析 (2016)
  • Author(s): 矢木宏和
  • Organizer: 日本大学理工学部インフォーマルセミナー
  • Place of Presentation: 日本大学理工学部（東京）
  • Year and Date: 2016-01-22
  • Invited
• [Presentation] Functional analysis of causative gene products of α-dystroglycanopaty associated with the formation of laminin-binding glycans (2016)
  • Author(s): Hirokazu Yagi
  • Organizer: The 3rd International Symposium on Glyco-Neuroscience
  • Place of Presentation: 淡路夢舞台（淡路）
  • Year and Date: 2016-01-15
  • Int'l Joint Research / Invited
• [Presentation] Extracellular O-GlcNAc regulates Dll4-dependent Notch signaling in the endothelial cells and BBB/BRB maintenance (2016)
  • Author(s): Mitsutaka Ogawa, Shogo Sawaguchi, Kyosuke Takeshita, Toyoaki Murohara, Hirokazu Yagi, Koichi Kato, Koichi Furukawa, and Tetsuya Okajima
  • Organizer: The 3rd International Symposium on Glyco-Neuroscience
  • Place of Presentation: 淡路夢舞台（淡路）
  • Year and Date: 2016-01-14
  • Int'l Joint Research
• [Presentation] Extracellular O-GlcNAc modification regulates Notch signaling and blood-retina barrier maintenance (2015)
  • Author(s): 小川光貴，澤口翔伍，Bieniasz-Krzywiec Pawel，矢木宏和，加藤晃一，臼倉治郎，古川鋼一，岡島徹也
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会（BMB2015）
  • Place of Presentation: 神戸ポートアイランド（神戸）
  • Year and Date: 2015-12-03
• [Presentation] 細胞外 O-GlcNAc の修飾に関わる EOGT 変異マウスの表現型解析 (2015)
  • Author(s): 澤口翔伍、小川光貴、矢木宏和、加藤晃一、臼倉治郎、古川鋼一、岡島徹也
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会（BMB2015）
  • Place of Presentation: 神戸ポートアイランド（神戸）
  • Year and Date: 2015-12-03
• [Presentation] Functional analysis of enzymes involved in the formation of laminin-binding glycans displayed on α-dystroglycan (2015)
  • Author(s): Hirokazu Yagi and Koichi Kato
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会（BMB2015）
  • Place of Presentation: 神戸ポートアイランド（神戸）
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] 硫酸化糖鎖は末梢神経系髄鞘の形成を制御する (2015)
  • Author(s): 吉村 武，半田(鳴海)麻衣，林 明子，矢木宏和，内村健治，門松健治、加藤晃一，Bruce D. Trapp，馬場広子，池中一裕
  • Organizer: 糖鎖科学中部拠点第12回「若手の力」フォーラム
  • Place of Presentation: 名城大学薬学部（名古屋）
  • Year and Date: 2015-11-28
• [Presentation] 細胞外O-GlcNAcの修飾に関わるEOGT変異マウスの表現形解析 (2015)
  • Author(s): Shogo Sawaguchi, Mitsutaka Ogawa, Hirokazu Yagi, Koichi Kato, Jiro Usukura, Koichi Furukawa, and Tetsuya Okajima
  • Organizer: 糖鎖科学中部拠点第12回「若手の力」フォーラム
  • Place of Presentation: 名城大学薬学部（名古屋）
  • Year and Date: 2015-11-28
• [Presentation] Application of N-glycosylation profiling by using HPLC mapping (2015)
  • Author(s): Hirokazu Yagi
  • Organizer: 糖鎖科学中部拠点/日本ウォーターズ共催セミナー
  • Place of Presentation: ウインクあいち（名古屋）
  • Year and Date: 2015-08-03
  • Invited
• [Presentation] GlcNAc6ST-1 は N 結合型糖鎖の硫酸化を介して末梢神経系の髄鞘形成を制御する (2015)
  • Author(s): 吉村 武，林 明子，内村健治，門松健治，矢木宏和，加藤晃一，馬場広子，池中一裕
  • Organizer: 第34回日本糖質学会年会
  • Place of Presentation: 東京大学（東京）
  • Year and Date: 2015-08-02
• [Presentation] 神経系における糖鎖の機能解明のための糖鎖プロファイリング技術の開発とその応用 (2015)
  • Author(s): 矢木宏和
  • Organizer: 第34回日本糖質学会年会
  • Place of Presentation: 東京大学（東京）
  • Year and Date: 2015-08-01
  • Invited
• [Presentation] Biological roles of extracellular O-GlcNAc in Notch signaling,vascular development,and blood retinal barrier maintenance (2015)
  • Author(s): Mitsutaka Ogawa, Shogo Sawaguchi, Pawel Bieniasz-Krzywiec, Hirokazu Yagi, Koichi Kato, Jiro Usukura, Koichi Furukawa, and Tetsuya Okajima
  • Organizer: 第79回日本生化学会中部支部例会
  • Place of Presentation: 信州大学松本キャンパス（松本）
  • Year and Date: 2015-05-23
• [Presentation] 細胞外O-GlcNAcの修飾に関わるEOGT変異マウスの表現型解析 (2015)
  • Author(s): Shogo Sawaguchi, Mitsutaka Ogawa, Hirokazu Yagi, Koichi Kato, Jiro Usukura, Koichi Furukawa, and Tetsuya Okajima
  • Organizer: 第79回日本生化学会中部支部例会
  • Place of Presentation: 信州大学松本キャンパス（松本）
  • Year and Date: 2015-05-23
• [Presentation] HPLCおよび質量分析による糖鎖の構造解析 (2015)
  • Author(s): 矢木宏和
  • Organizer: 技術情報協会セミナー「バイオ/抗体医薬品における糖鎖・タンパク質解析の 基礎とバリデーション」
  • Place of Presentation: 技術情報協会（東京）
  • Year and Date: 2015-05-19
  • Invited
• [Book] 糖鎖の新機能開発・応用ハンドブック～創薬・医療から食品開発まで～ (2015)
  • Author(s): 矢木宏和，加藤晃一
  • Total Pages: 6
  • Publisher: ㈱エヌ・ティー・エス
• [Remarks] 研究室ホームページ
  • URL: http://www.phar.nagoya-cu.ac.jp/hp/sbk/kenkyu/index.html