Selective elimination of tumor clones of primary myelofibrosis based on drug repositioning

• Project/Area Number: 15K07972
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pharmacology in pharmacy
• Research Institution: Juntendo University
• Principal Investigator: 後藤 明彦 順天堂大学, 医学部, 先任准教授 (00297293)
• Co-Investigator(Kenkyū-buntansha): 小松 則夫 順天堂大学, 医学部, 教授 (50186798) ; 宮澤 啓介 東京医科大学, 医学部, 主任教授 (50209897)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: myelofibrosis / ruxolitinib / macrolide / thrombopietin / calreticulin / autophagy / 原発性骨髄線維症 / オートファジー / ルキソリチニブ / マクロライド

Outline of Annual Research Achievements

原発性骨髄線維症（PMF）の主要なドライバー変異であるcalreticulin（CALR）のtype1（del52）およびtype2（ins5）の変異遺伝子をPMFの病態の核となる巨核球系細胞のモデルとなるthrombopoietin（TPO）依存性細胞株UT-7/TPOに導入し、安定的変異遺伝子導入株を得た。ベクター遺伝子と野生型CALR遺伝子導入株をコントロールとして作成した。変異遺伝子導入株はTPO非依存性の増殖能を獲得しており、PMFの腫瘍性性格を反映していると考えられた。
クラリスロマイシン（CAM）は単独ではCALR変異陽性細胞に対する増殖抑制効果を示さなかったが、ルキソリチニブ（RUX）との併用によりRUXの細胞増殖抑制作用を増強した。なおCALR type2変異導入のクローンのうち1種ではRUXに高い耐性を示した。細胞内タンパクのチロシンリン化状態を検討するとRUX添加でリン酸化は効果的に抑制されたがCAM単独ではリン酸化抑制は認めず、RUXとCAMの併用でもRUX単独と著変はなく、CAMのRUXによる増殖抑制効果増強はJAK2の恒常的活性化によるチロシンリン酸化のレベルとは関係ないことが示された。また、RUX抵抗性を示したクローンではRUXによるチロシンリン酸化抑制が認められず、RUX耐性機序の一つとして記憶すべきと考えた。
無血清の条件下でLC3-IIの動態を詳細に観察するとCALR変異細胞では無血清下4時間までLC3-IIが経時的に増加するのが観察され、オートファジーが経時的に誘導されるのが示された。オートファジーを阻害するCAMはここをターゲットとしてRUXの作用を増強している可能性があり、安全性の確立したマクロライドのドラッグリポジショニングによるRUXとの併用はRUXの臨床効果を高めるのに有望な選択と考えられた。

Research Products

• [Journal Article] Evidence for prevention of renal dysfunction associated with primary myelofibrosis by cytoreductive therapy (2019)
  • Author(s): Fukuda Yasutaka、Araki Marito、Yamamoto Kouji、Morishita Soji、Inano Tadaaki、Misawa Kyohei、Ochiai Tomonori、Edahiro Yoko、Imai Misa、Yasuda Hajime、Gotoh Akihiko、Ohsaka Akimichi、Komatsu Norio
  • Journal Title: Haematologica Volume: 印刷中
  • DOI: 10.3324/haematol.2018.208876
  • Peer Reviewed / Open Access
• [Journal Article] Hommomultimerization of mutant calreticulin is a prerequisite for MPL binding and activation. (2019)
  • Author(s): Araki Marito、Yang Yinjie、Imai Misa、Mizukami Yoshihisa、Kihara Yoshihiko、Sunami Yoshitaka、Masubuchi Nami、Edahiro Yoko、Hironaka Yumi、Osaga Satoshi、Ohsaka Akimichi、Komatsu Norio
  • Journal Title: Leukemia Volume: 33 Pages: 122-131
  • DOI: 10.1038/s41375-018-0181-2
  • Peer Reviewed / Open Access
• [Journal Article] Transfusion independence achieved with pomalidomide therapy in a patient with primary myelofibrosis (2018)
  • Author(s): 枝廣 陽子, 後藤 明彦, 稲野 資明, 筒井 深雪, 築根 豊, 安田 肇, 小松 則夫
  • Journal Title: Jpn. J. Clin. Hematol. Volume: 59 Issue: 3 Pages: 323-325
  • DOI: 10.11406/rinketsu.59.323
  • NAID: 130006627427
  • Peer Reviewed
• [Journal Article] Mutational subtypes of JAK2 and CALR correlate with different clinical features in Japanese patients with myeloproliferative neoplasms (2018)
  • Author(s): Misawa K, Yasuda H, Araki M, Ochiai T, Morishita S, Shirane S, Edahiro Y, Gotoh A, Ohsaka A, Komatsu N.
  • Journal Title: Int J Hematol. Volume: 印刷中 Pages: 673-680
  • DOI: 10.1007/s12185-018-2421-7
  • Peer Reviewed
• [Journal Article] The 2014 BCSH criteria and the 2016 WHO criteria for essential thrombocythemia: A comparison in a large-scale cohort (2018)
  • Author(s): Ochiai T, Yasuda H, Araki M, Misawa K, Morishita S, Nudejima M, Hironaka Y, Shirane S, Edahiro Y, Gotoh A, Ohsaka A, Komatsu N.
  • Journal Title: Eur J Haematol. Volume: 印刷中 Pages: 544-549
  • DOI: 10.1111/ejh.13041
  • Peer Reviewed
• [Journal Article] Designing an effective drug combination for ER stress loading in cancer therapy using a real-time monitoring system. (2018)
  • Author(s): Kazama H, Hiramoto M, Miyahara K, Takano N, Miyazawa K.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: - Pages: 286-292
  • DOI: 10.1016/j.bbrc.2018.05.001
  • Peer Reviewed / Open Access
• [Journal Article] Amino acid starvation culture condition sensitizes EGFR-expressing cancer cell lines to gefitinib-mediated cytotoxicity by inducing atypical necroptosis (2018)
  • Author(s): Saito Y, Moriya S, Kazama H, Hirasawa K, Miyahara K, Kokuba H, Hino H, Kikuchi H, Takano N, Hiramoto M, Tsukahara K, Miyazawa K.
  • Journal Title: International Journal of Oncology Volume: 52 Pages: 1165-1177
  • DOI: 10.3892/ijo.2018.4282
  • Peer Reviewed / Open Access
• [Journal Article] Mutant molecular chaperone activates cytokine receptor as a homomultimer (2018)
  • Author(s): Araki Marito、Komatsu Norio
  • Journal Title: Oncotarget Volume: 9 Pages: 35201-35202
  • DOI: 10.18632/oncotarget.26221
  • Peer Reviewed / Open Access
• [Journal Article] Multiple Placental Infarcts in a Pregnant Woman with Essential Thrombocythemia (2018)
  • Author(s): Edahiro Yoko、Ando Jun、Suzuki Toshifumi、Fukumura Yuki、Masuda Azuchi、Sakayori Shiori、Takeda Jun、Maruyama Yojiro、Makino Shintaro、Itakura Atsuo、Komatsu Norio
  • Journal Title: Intern. Med. Volume: 57 Issue: 24 Pages: 3647-3650
  • DOI: 10.2169/internalmedicine.1311-18
  • NAID: 130007432644
  • Peer Reviewed
• [Journal Article] Skewed megakaryopoiesis in human induced pluripotent stem cell-derived haematopoietic progenitor cells harbouring calreticulin mutations. (2018)
  • Author(s): Hiraku Takei, Yoko Edahiro, Shuichi Mano, Nami Masubuchi, Yoshihsa Mizukami, Misa Imai, Soji Morishita, Kyohei Misawa, Tomonori Ochiai, Satoshi Tsuneda, Hiroshi Endo, Sou Nakamura, Koji Eto, Akimichi Ohsaka, Marito Araki, Norio Komatsu
  • Journal Title: Br J Haematol. Volume: 印刷中 Pages: 791-802
  • DOI: 10.1111/bjh.15266
  • Peer Reviewed
• [Journal Article] Effect of ruxolitinib therapy on the quality-of-life of Japanese patients with myelofibrosis (2018)
  • Author(s): Oritani K, Ohishi K, Okamoto S, Kirito K, Komatsu N, Tauchi T, Handa H, Saito S, Takenaka K, Shimoda K, Okada H, Amagasaki T, Wakase S, Shimozuma K, Akashi K
  • Journal Title: Current Medical Research and Opinion Volume: 34 Pages: 531-537
  • DOI: 10.1080/03007995.2017.1415874
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Meet the MPN experts in EHA フィラデルフィア染色体陰性MPNの診断・治療の課題と将来展望 MPNを巡る臨床的課題と将来展望を語る(座談会) (2018)
  • Author(s): 小松 則夫, Kiladjian Jean-Jacques, ROY Lydia, Shih Lee-Yung, Chen Chin-Cheng, 後藤 明彦
  • Journal Title: 血液フロンティア Volume: 28 Pages: 263-267
• [Journal Article] Meet the MPN experts in EHA フィラデルフィア染色体陰性MPNの診断・治療の課題と将来展望 MPNを巡る臨床的課題と将来展望を語る (2018)
  • Author(s): 小松則夫, Kiladjan JJ, Roy L, Shih L-Y, Chen C-C, 後藤明彦
  • Journal Title: 血液フロンティア Volume: 28 Pages: 263-267
• [Journal Article] Gene mutations in myeloproliferative neoplasms (2017)
  • Author(s): 荒木 真理, 森下 総司, 小松 則夫
  • Journal Title: Jpn. J. Clin. Hematol. Volume: 12 Issue: 12 Pages: 2526-2534
  • DOI: 10.11406/rinketsu.57.2526
  • NAID: 130005286786
  • Year and Date: 2017-01-13
  • Peer Reviewed
• [Journal Article] The 2016 WHO diagnostic criteria for polycythemia vera renders an accurate diagnosis to a broader range of patients including masked polycythemia vera: comparison with the 2008 WHO diagnostic criteria. (2017)
  • Author(s): Misawa K, Yasuda H, Araki M, Ochiai T, Morishita S, Nudejima M, Hironaka Y, Shirane S, Edahiro Y, Gotoh A, Ohsaka A, Komatsu N.
  • Journal Title: American journal of hematology Volume: 92
  • DOI: 10.1002/ajh.24752
  • Peer Reviewed
• [Journal Article] 骨髄増殖性腫瘍(MPN)の分子病態と診断・治療のup-to-date】 Ph陰性MPNの予後・予後予測因子 (2017)
  • Author(s): 後藤明彦
  • Journal Title: 最新医学 Volume: 72 Pages: 1565-1571
• [Journal Article] Assessing the safety and efficacy of ruxolitinib in a multicenter, open-label study in Japanese patients with myelofibrosis. (2017)
  • Author(s): Komatsu N, Kirito K, Shimoda K, Ishikawa T, Ohishi K, Ohyashiki K, Takahashi N, Okada H, Amagasaki T, Yonezu T, Akashi K.
  • Journal Title: Int J Hematol Volume: 105 Pages: 309-317
  • DOI: 10.1007/s12185-016-2130-z
  • Peer Reviewed
• [Journal Article] Macrolide antibiotics exhibit cytotoxic effect under amino acid-depleted culture condition by blocking autophagy flux in head and neck squamous cell carcinoma cell lines. (2016)
  • Author(s): Hirasawa K, Moriya S, Miyahara K, Kazama H, Hirota A, Takemura J, Abe A, Inazu M, Hiramoto M, Tsukahara K, Miyazawa K.
  • Journal Title: PLoS One. Volume: 11 Pages: 1848-1858
  • DOI: 10.1371/journal.pone.0164529
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Targeting bortezomib-induced aggresome formation using vinorelbine enhances the cytotoxic effect along with ER stress loading in breast cancer cell lines. (2016)
  • Author(s): Miyahara K, Kazama H, Kokuba H, Komatsu S, Hirota A, Takemura J, Hirasawa K,Moriya S, Abe A, Hiramoto M, Ishikawa T, Miyazawa K.
  • Journal Title: Int J Oncol Volume: 49 Pages: 1848-1858
  • DOI: 10.3892/ijo.2016.3673
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 骨髄増殖性腫瘍をひき起こす変異型calreticulinによるTPO受容体の活性化機構 (2016)
  • Author(s): 今井 美沙, 荒木 真理人, 森下 総司, 小松 則夫
  • Journal Title: 血液内科 Volume: 73 Pages: 789-793
  • NAID: 40021031814
• [Journal Article] 診断における遺伝子変異の意義と問題点 (2016)
  • Author(s): 小松 則夫
  • Journal Title: 日本検査血液学会雑誌 Volume: 17 Pages: 347-353
• [Journal Article] Targeting bortezomib-induced aggresome formation using vinorelbine enhances the cytotoxic effect along with ER stress loading in breast cancer cell lines. (2016)
  • Author(s): Mukai S, Moriya S, Hiramoto M, Kazama H, Kokuba H, Che XF, Yokoyama T, Sakamoto S, Sugawara A, Sunazuka T, Omura S, Handa H, Itoi T, Miyazawa K.
  • Journal Title: Int. J. Clin. Oncol. Volume: 48 Pages: 1848-1858
  • DOI: 10.3892/ijo.2015.3237
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Activation of the thrombopoietin receptor by mutant calreticulin in CALR-mutant myeloproliferative neoplasms. (2016)
  • Author(s): Araki M, Yang Y, Masubuchi N, Hironaka Y, Takei H, Morishita S, Mizukami Y, Kan S, Shirane S, Edahiro Y, Sunami Y, Ohsaka A, Komatsu N
  • Journal Title: Blood Volume: 127 Pages: 1307-1316
  • DOI: 10.1182/blood-2015-09-671172
  • Peer Reviewed / Open Access
• [Journal Article] Targeting the integrated networks of aggresome formation, proteasome, and autophagy potentiates ER stress‑mediated cell death in multiple myeloma cells. (2015)
  • Author(s): Moriya S, Komatsu S, Yamasaki K, Kawai Y, Kokuba H, Hirota A, Che XF, Inazu M, Gotoh A, Hiramoto M, Miyazawa K
  • Journal Title: Int J Oncol Volume: Feb;46(2) Pages: 474-486
  • DOI: 10.3892/ijo.2014.2773
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Usefulness of BCSH Criteria for Diagnosing Japanese Polycythemia Vera -Comparative Analysis with WHO 2008 Criteria- (2015)
  • Author(s): Yahata Y, Gotoh A, Komatsu N
  • Journal Title: Juntendo Medical Journal Volume: 61 Pages: 287-293
  • Peer Reviewed
• [Journal Article] Reactivation of hepatitis B virus during treatment with hydroxyurea in an elderly patient with essential thrombocythemia. (2015)
  • Author(s): Watanabe N, Yasuda H, Aota Y, Ando J, Aoyama T, Tanaka M, Gotoh A, Komatsu N.
  • Journal Title: Geriatr Gerontol Int Volume: 15 Pages: 812-3
  • DOI: 10.1111/ggi.12461
  • Peer Reviewed
• [Journal Article] EGFR-independent autophagy induction with gefitinib and enhancement of its cytotoxic effect by targeting autophagy with clarithromycin in non-small cell lung cancer cells. (2015)
  • Author(s): Sugita S, Ito K, Yamashiro Y, Moriya S, Che XF, Yokoyama T, Hiramoto M, Miyazawa K.
  • Journal Title: Biochem Biophys Res Commun Volume: Apr 7 Pages: 28-34
  • DOI: 10.1016/j.bbrc.2015.03.162
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Melting curve analysis after T allele enrichment (MelcaTle) as a highly sensitive and reliable method for detecting the JAK2V617F mutation (2015)
  • Author(s): Morishita S, Takahashi K, Araki M, Hironaka Y, Sunami Y, Edahiro Y, Tsutsui M, Ohsaka A, Tsuneda S, Komatsu N.
  • Journal Title: PLOS One Volume: 10
  • DOI: 10.1371/journal.pone.0122003
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Consequences of the JAK2V617F allele burden for the prediction of transformation into myelofibrosis from polycythemia vera and essential thrombocythemia. (2015)
  • Author(s): Shirane S, Araki M, Morishita S, Edahiro Y, Sunami Y, Hironaka Y, Noguchi M, Koike M, Sato E, Ohsaka A, Komatsu N.
  • Journal Title: Int J Hematol. Volume: 101 Pages: 148-53
  • DOI: 10.1007/s12185-014-1721-9
  • Peer Reviewed
• [Journal Article] Current problems in the diagnosis of Philadelphia-negative myeloproliferative neoplasms in Japan (2015)
  • Author(s): 白根脩一、荒木真理人、森下総司、枝廣陽子、大阪顯通、小松則夫
  • Journal Title: Jpn. J. Clin. Hematol. Volume: 56 Issue: 7 Pages: 877-82
  • DOI: 10.11406/rinketsu.56.877
  • NAID: 130005093120
  • Peer Reviewed
• [Presentation] Copy number of JAK2V617 modulates human hematopoietic cell differentiation . (2018)
  • Author(s): Edahiro Y, Mano S, Takei H, Li L, Morishita S, Gotoh A, Tsuneta S, Osaka A, Araki M, Komatsu N
  • Organizer: 8th International Conference on Myeloproliferative Neoplasms
  • Int'l Joint Research
• [Presentation] Clinical and molecular features of patients with triple-negative essential thrombocythemia. (2018)
  • Author(s): Inano T, Araki M, Fukuda Y, Morishita S, Ochiai T, Misawa K, Ito M, Yamamoto K, Yang Y, Taguchi T, Edahiro Y, Imai M, Gotoh A, Osaka A, Komatsu N.
  • Organizer: 第80回日本血液学会学術集会
• [Presentation] Cytoreductive therapy prevents worsening of renal function in patients with primary myelofibrosis. (2018)
  • Author(s): Fukuda Y, Araki M, Imamoto K, Morishita S, Inano T, Misawa K, Ochiai T, Edahiro Y, Imai M, Gotoh A, Osaka A, Komatsu N.
  • Organizer: 第80回日本血液学会学術集会
• [Presentation] Interferon therapy for pregnant patients with myeloproliferative neoplasms. (2018)
  • Author(s): Edahiro Y, Gotoh A, Ando J, Tsutsui M, Morishita S, Osaka A, Komatsu N.
  • Organizer: 第80回日本血液学会学術集会
• [Presentation] Myeloproliferative neoplasm with BCR-JAK2 fusion (2017)
  • Author(s): Tsutsui M, Gotoh , Morishita S, Araki M, Fukuda Y, Nitta H, Ando J, Komatsu N.
  • Organizer: 第79回日本血液学会学術集会
• [Presentation] Treatment for myelodysplastic syndrome: with a focus on the low-risk group (2017)
  • Author(s): Gotoh A
  • Organizer: 第79回日本血液学会学術集会
  • Invited
• [Presentation] Clinical characteristics of BCSH criteria defined PV and ET patients in Japan (2016)
  • Author(s): Ochiai M, Araki M, Misawa K, Morishita S, Nudejima M, Hironaka Y, Shirane S, Edahiro Y, Sunami Y, Goth A, Osaka A, Komatsu N
  • Organizer: 第78回日本血液学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-10-13
• [Presentation] Impact of mutations on efficacy of anagrelide in Japanese patients with essential thrombocythemia (2016)
  • Author(s): Tsutsui M, Gotoh, A Araki M, Morishita S, Komatsu N
  • Organizer: 第78回日本血液学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-10-13
• [Presentation] Evaluation of the dose and efficacy of ruxolitinib in Japanese patients with myelofibrosis (2016)
  • Author(s): Kirito K, Okamoto S, Shishi K, Tauchi T, Handa H, Saito S, Takenaka K, Shimoda K, Ogitani K, Komatsu N, Okada H, Amagasaki T, Suzuki K Yonezo T, Akashi K
  • Organizer: 第78回日本血液学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-10-13
• [Presentation] Differential clinical features by subtypes of Calreticulin mutation in myeloproliferative neoplasms (2016)
  • Author(s): Misawa K, Araki M, Ochiai T, Morishita S, Nudejima M, Hironaka Y, Shirane S, Edahiro Y, Sunami S, Gotoh A, Osaka A, Komatsu N
  • Organizer: 第78回日本血液学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-10-13
• [Presentation] Modeling MPN phenotypes in vitro by using iPSC harboring CALR ins5 mutation (2016)
  • Author(s): Takei T, Masubuchi N, Mano S, Araki M, Morishita S, Mizukami Y, Kan S, Edahiro Y, Sunami Y, Hironaka Y, Endo H, Nakamura S, Eto K, Osaka A, Komatsu N
  • Organizer: 第78回日本血液学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-10-13
• [Presentation] Mutant calreticulin activates the thrombopoietin receptor in myeloproliferative neoplasms (2016)
  • Author(s): Araki M, Yang Y, Masubuchi N, Hironaka Y, Takei H, Morishita S, Mizukami Y, Kan S, Shirane S, Edahiro Y, Sunami Y, Osaka A, Komatsu N
  • Organizer: 第78回日本血液学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-10-13
• [Presentation] Macrolides enhance bortezomib-induced cytotoxicity in myeloma cells co-cultured with stroma layer (2016)
  • Author(s): Morishita S, Kzazma H, Hiramoto M, Aizawa S, Sunazuka T, Handa H, Miyazawa K
  • Organizer: 第78回日本血液学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-10-13
• [Presentation] Cell surface localization of mutant calreticulin that induces myeloproliferative neoplasms (MONs) (2016)
  • Author(s): Masubuchi N, Araki M, Yang Y, Hironaka Y, Takei H, Morishita S, Mizukami Y, Kan S, Osaka A, Komatsu N
  • Organizer: 第78回日本血液学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-10-13
• [Presentation] 骨髄増殖性腫瘍細胞において変異型Calreticulinはトロンボポエチン受容体と相互作用しJAK2を活性化する (2016)
  • Author(s): 荒木 真理人, 小松 則夫
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-10-06
• [Presentation] アミノ酸飢餓状態下で、マクロライド系抗菌薬はオートファジー阻害により頭頸部癌細胞株の細胞死を誘導する (2016)
  • Author(s): 平澤 一浩, 森谷 昇太, 宮原 か奈, 平本 正樹, 塚原 清彰, 宮澤 啓介
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-10-06
• [Presentation] ビノレルビンは乳癌細胞株においてアグリソーム形成を阻害することによりボルテゾミブ誘導性の細胞毒性を増強する (2016)
  • Author(s): 宮原 か奈, 森谷 昇太, 小松 誠一郎, 平澤 一浩, 阿部 晃久, 平本 正樹, 石川 孝, 宮澤 啓介
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-10-06
• [Presentation] Activation of AP-1 By JAK2V617F through Induction and Stabilization of c-FOS (2015)
  • Author(s): Yoko Edahiro, Marito Araki, Yumi Hironaka, Yinjie Yang, Soji Morishita, Yoshitaka Sunami, Akimichi Ohsaka, Norio Komatsu
  • Organizer: 第57回米国血液学会議
  • Place of Presentation: Orland,Florida, USA
  • Year and Date: 2015-12-05
  • Int'l Joint Research
• [Presentation] Assessing the Safety and Efficacy of Ruxolitinib in a Multicenter, Open-Label, Expanded-Access Study in Japanese Patients with Myelofibrosis (MF) (2015)
  • Author(s): Keita Kirito, Norio Komatsu, Kazuya Shimoda, Hikaru Okada, Taro Amagasaki, Toshio Yonezu, Koichi Akashi
  • Organizer: 第57回米国血液学会議
  • Place of Presentation: Orland,Florida, USA
  • Year and Date: 2015-12-05
  • Int'l Joint Research
• [Presentation] Establishment of iPS cells from myeloproliferative neoplasms patients with CALR mutation (2015)
  • Author(s): 竹井 拓, 森下 総司, 荒木 真理人, 水上 喜久, 枝廣 陽子, 角南 義孝, 弘中 由美, 津村 真知子, 常田 聡, 遠藤 大, 中村 壮, 江藤 浩之, 大坂 顯通, 小松 則夫
  • Organizer: 第77回日本血液学会学術集会
  • Place of Presentation: 石川県立音楽堂、ANAクラウンプラザホテル金沢、ホテル日航金沢、ホテル金沢、金沢市アートホール、もてなしドーム（石川県、金沢市）
  • Year and Date: 2015-10-16
• [Presentation] Early disease onset of patients with triple-negative myeloproliferative neoplasms (2015)
  • Author(s): 白根 脩一, 荒木 真理人, 森下 総司, 枝廣 陽子, 竹井 拓, 津村 真知子, 弘中 由美, 落合 友則, 三澤 恭平, 大坂 顯通, 小松 則夫
  • Organizer: 第77回日本血液学会学術集会
  • Place of Presentation: 石川県立音楽堂、ANAクラウンプラザホテル金沢、ホテル日航金沢、ホテル金沢、金沢市アートホール、もてなしドーム（石川県、金沢市）
  • Year and Date: 2015-10-16
• [Presentation] A highly specific method for detecting a single copy JAK2V617F mutation (2015)
  • Author(s): 森下 総司, 荒木 真理人, 弘中 由美, 角南 義孝, 枝廣 陽子, 筒井 深雪, 大坂 顯通, 小松 則夫
  • Organizer: 第77回日本血液学会学術集会
  • Place of Presentation: 石川県立音楽堂、ANAクラウンプラザホテル金沢、ホテル日航金沢、ホテル金沢、金沢市アートホール、もてなしドーム（石川県、金沢市）
  • Year and Date: 2015-10-16
• [Presentation] Trangeting autophagy with clarithromycin enhances gefitinib-induced cell death in non-small cell lung cancer cells (2015)
  • Author(s): 森谷昇太、車暁芳、横山智央、平本正樹、宮澤啓介
  • Organizer: 第74回 日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（愛知県、名古屋市）
  • Year and Date: 2015-10-08
• [Book] 病気がみえる vol.5 血液 骨髄系腫瘍 (2017)
  • Author(s): 後藤明彦 監修
  • Publisher: メディックメディア
• [Book] 骨髄線維症 小松則夫編 第3章 骨髄線維症の診断と予後 1.診断 (2016)
  • Author(s): 後藤明彦
  • Publisher: 医薬ジャーナル社