Basic, practical and pioneer research on the treatment of allergic diseases using antigen-specific monoclonal IgA

• Project/Area Number: 15K07985
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pharmacology in pharmacy
• Research Institution: Kobe Pharmaceutical University
• Principal Investigator: Yamaki Kouya 神戸薬科大学, 薬学部, 准教授 (00351768)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: IgA / immunosuppression / Immunosuppression / 免疫抑制 / アレルギー

Outline of Final Research Achievements

To investigate the antigen-specific immunosuppressive roles of IgA on various species, we determined the DNA sequences of variable regions of mouse anti-ovalbumin IgA, which showed immunosuppressive effect in mice. Then the antibody secreting cell was established by transfecting the human IgA2 and kappa constant regions expressing vectors inserted with the sequences of the determined variable regions. In addition, we clarified that the immunosuppressive effects of mouse IgA on immune reaction in mice were, at least partly, mediated through antigen neutralization blunting B cell activation.

Research Products

• [Journal Article] Interdisciplinary Subject “Yakugaku Nyumon” for First-year Students Constructed with Lectures and Problem-based Learning (2016)
  • Author(s): Yamaki K, Ueda M, Ueda K, Emoto N, Mizutani N, Ikeda K, Yagi K, Tanaka M, Habu Y, Nakayama Y, Takeda N, Moriwaki K, Kitagawa S.
  • Journal Title: YAKUGAKU ZASSHI Volume: 136 Issue: 7 Pages: 1051-1064
  • DOI: 10.1248/yakushi.15-00255
  • NAID: 130005159464
  • ISSN: 0031-6903, 1347-5231
  • Year and Date: 2016-07-01
  • Peer Reviewed
• [Journal Article] ラットの卵白アルブミンへの感作に対するマウス抗卵白アルブミンＩｇＡの抑制作用 (2016)
  • Author(s): 八巻 耕也、吉野 伸
  • Journal Title: 応用薬理 Volume: ９０ Pages: 41-45
  • Peer Reviewed
• [Journal Article] Interdisciplinary Subject “YakugakuNyumon” for First-Year Students Constructed with Lectures and Problem-Based Learning (2016)
  • Author(s): K Yamaki, M Ueda, K Ueda, N Emoto, N Mizutani, K Ikeda, K Yagi, M Tanaka, Y Habu, Y Nakayama, N Takeda, K Moriwaki, S Kitagawa
  • Journal Title: Yakugaku Zasshi Volume: -
  • NAID: 130005159464
  • Peer Reviewed
• [Journal Article] ラットの卵白アルブミンへの感作に対するマウス抗卵白アルブミン IgA の抑制作用 (2016)
  • Author(s): 八巻 耕也,吉野 伸
  • Journal Title: 応用薬理 Volume: -
  • Peer Reviewed
• [Journal Article] IgA directly inhibits antigen-dependent B cell activation following distinctive distribution of the antigen in mice (2016)
  • Author(s): K Yamaki, S Yoshino.
  • Journal Title: Immunopharmacol. Immunotoxicol. Volume: 38 Issue: 2 Pages: 131-144
  • DOI: 10.3109/08923973.2016.1142559
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A new, rapid in vivo method to evaluate allergic responses through distinctive distribution of a fluorescent-labeled immune complex: Potential to investigate anti-allergic effects of compounds administered either systemically or topically to the skin (2016)
  • Author(s): K Yamaki, S Yoshino.
  • Journal Title: J. Immunol. Methods Volume: 428 Pages: 58-68
  • DOI: 10.1016/j.jim.2015.11.010
  • Peer Reviewed
• [Journal Article] Establishment of a mouse anti-ovalbumin IgE monoclonal antibody that induces FcεRII (CD23)-dependent activation without FcεRI-dependent activation (2015)
  • Author(s): K Yamaki, S Yoshino.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 34 Issue: 6 Pages: 423-431
  • DOI: 10.1089/mab.2015.0032
  • Peer Reviewed
• [Journal Article] 3-Chloro-1,2-propanediol (3-MCPD), a carcinogenic food contaminant, inhibits mast cell activation via affecting Ca2+ mobilization (2015)
  • Author(s): K Yamaki, S Yoshino.
  • Journal Title: Oyo Yakuri/Pharmacometrics Volume: 88 Pages: 87-95
  • Peer Reviewed
• [Journal Article] The therapeutic potential of monoclonal IgA antibodies in allergic diseases: Suppressive effect of IgA on immune responses induced by re-exposure to antigen in sensitized mice by a monoclonal IgE antibody that binds to a different epitope of the same antigen (2015)
  • Author(s): K Yamaki, S Yoshino.
  • Journal Title: Monoclon. Antib. Immunodiagn. Immunother. Volume: 34 Issue: 2 Pages: 83-89
  • DOI: 10.1089/mab.2014.0078
  • Peer Reviewed
• [Presentation] IgA の抗原特異的な感作抑制作用 (2017)
  • Author(s): 八巻 耕也、吉野 伸
  • Organizer: 日本薬学会 第１３７年会
  • Place of Presentation: 仙台
• [Presentation] 抗原の特徴的分布 (ASDIS) を指標とする簡便で迅速な新規アレルギーマウスモデルの確立 (2016)
  • Author(s): 八巻 耕也、吉野 伸
  • Organizer: 日本薬学会 第１３６年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-03-27
• [Presentation] 初年次生に対する分野横断的統合型教育科目 「薬学入門」の学習効果 (2016)
  • Author(s): 土生 康司、八巻 耕也、上田 昌史、上田 久美子、江本 憲昭、水谷 暢明、池田 宏二、八木 敬子、田中 将史、中山 喜明、武田 紀彦、森脇 健介、北河 修治
  • Organizer: 日本薬学会 第１３６年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-03-27
• [Presentation] IgA の抗原特異的な感作抑制作用 (2015)
  • Author(s): 八巻 耕也、吉野 伸
  • Organizer: 第１２８回 日本薬理学会近畿部会
  • Place of Presentation: 大阪
  • Year and Date: 2015-11-20
• [Presentation] In vivo imaging を用いた新規マウスアレルギー反応迅速測定法 (2015)
  • Author(s): 八巻 耕也、吉野 伸
  • Organizer: 第１９回日本ヒスタミン学会
  • Place of Presentation: 仙台
  • Year and Date: 2015-11-14