| Project/Area Number |
15K07990
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Natural medicines
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| Research Institution | University of Toyama |
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Principal Investigator |
Kurosaki Fumiya 富山大学, 大学院医学薬学研究部(薬学), 教授 (70143865)
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| Co-Investigator(Kenkyū-buntansha) |
加藤 康夫 富山県立大学, 工学部, 教授 (20254237)
荻田 信二郎 県立広島大学, 生命環境学部, 教授 (50363875)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 有用物質生産 / 遺伝子組み換え植物 / 芳香性セスキテルペン / 植物二次代謝 / Rac型GTPase / Rac-GTPase / 植物細胞工学 / 遺伝子組み換え / 植物 / バイオテクノロジー / 物質生産 / 遺伝子 / 有用資源 / 情報伝達機構 / rac GTPase |
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| Outline of Final Research Achievements |
δ-Guaiene is an aroma sesquiterpene compound accumulated in the trunk of Aquilaria microcarpa upon the microbial infection or mechanical wounding. We demonstrated that Rac-GTPase, a class of monomeric GTP binding protein, plays a central role in the transcriptional activation processes ofδ-guaiene synthetic enzyme gene (GS). We also showed that transformation of A. microcarpa with a modified gene of Rac-GTPase, CArac2, of which translate is capable of binging to GTP continuously, resulted in an appreciable expression of GS in the plant cells.
In order to construct the bio-production systems of δ-guaiene, GS plus farnesyl diphosphate synthase gene were co-expressed in Escherichia coli or tobacco BY2 cells. Upon the addition of mevalonolactone as the precursor, δ-guaiene contents in the transformed E. coli was 37.9 mg/L culture while 428 mg/L of the compound was accumulated in BY2 cells.
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