| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Outline of Final Research Achievements |
To elucidate the diterpene biosynthetic machinery of scopadulcic acid B (SDB), which are known for their unique tetracyclic skeleton in Scoparia dulcis, we performed an RNA-seq analysis. We found diterpene synthases genes including a putative syn-copalyl diphosphate synthase (SdCPS2) and a kaurene synthase-like (SdKSL1) genes. Besides them, more than 90 full-length of cytochrome P450 (CYP450) genes were also discovered. The expression analyses showed selected CYP450s associated with their expression pattern of SdCPS2 and SdKSL1, suggesting that CYP450 candidates involved diterpene modification. Functional analysis of the SdCPS2 revealed that SdCPS2 was suggested to be syn-CPS in S. dulcis. SdCPS2 represents the first predicted gene to produce syn-copalyl diphosphate in dicots. In addition, SdKSL1 potentially contributes to the SDB biosynthetic pathway.
|
