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Comprehensive studies for the biotechnological production of daurichromenic acid, an anti-HIV natural product

Research Project

Project/Area Number 15K07994
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Natural medicines
Research InstitutionUniversity of Toyama

Principal Investigator

Taura Futoshi  富山大学, 大学院医学薬学研究部(薬学), 准教授 (00301341)

Co-Investigator(Renkei-kenkyūsha) AKASHI Tomoyoshi  日本大学, 生物資源科学部, 准教授 (80328707)
MORITA Hiroyuki  富山大学, 和漢医薬学総合研究所, 教授 (20416663)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords生合成 / メロテルペノイド / ダウリクロメン酸 / バイオテクノロジー / エゾムラサキツツジ / プレニル転移酵素 / ポリケチド合成酵素
Outline of Final Research Achievements

In this project, I have attempted comprehensive understanding and elucidation of the biosynthetic genes of daurichromenic acid, a meroterpenoid with a potent anti-HIV activity isolated from a medicinal plant Rhododendron dauricum. I have successfully cloned genes coding for polyketide synthase, prenyltransferase, and oxidocyclase (DCA synthase) that constitute the DCA pathway. This study comprehensively identified the biosynthetic genes and established the heterologous expression system of each biosynthetic enzyme to develop the biotechnological production of daurichromenic acid.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (19 results)

All 2018 2017 2016 2015

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (14 results) (of which Int'l Joint Research: 4 results,  Invited: 4 results) Book (1 results)

  • [Journal Article] Daurichromenic acid and grifolic acid: Phytotoxic meroterpenoids that induce cell death in cell culture of their producer Rhododendron dauricum2018

    • Author(s)
      Taura F, Iijima M, Kurosaki F
    • Journal Title

      Plant Signaling & Behavior

      Volume: 13 Issue: 1 Pages: e1422463-e1422463

    • DOI

      10.1080/15592324.2017.1422463

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and Characterization of Daurichromenic Acid Synthase Active in Anti-HIV Biosynthesis2017

    • Author(s)
      Iijima Miu、Munakata Ryosuke、Takahashi Hironobu、Kenmoku Hiromichi、Nakagawa Ryuichi、Kodama Takeshi、Asakawa Yoshinori、Abe Ikuro、Yazaki Kazufumi、Kurosaki Fumiya、Taura Futoshi
    • Journal Title

      Plant Physiology

      Volume: 174 Issue: 4 Pages: 2213-2230

    • DOI

      10.1104/pp.17.00586

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Combinatorial Biosynthesis of (+)-Daurichromenic Acid and Its Halogenated Analogue2017

    • Author(s)
      Okada Masahiro、Saito Kai、Wong Chin Piow、Li Chang、Wang Dongmei、Iijima Miu、Taura Futoshi、Kurosaki Fumiya、Awakawa Takayoshi、Abe Ikuro
    • Journal Title

      Organic Letters

      Volume: 19 Issue: 12 Pages: 3183-3186

    • DOI

      10.1021/acs.orglett.7b01288

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A novel class of plant type III polyketide synthase involved in orsellinic acid biosynthesis from Rhododendron dauricum.2016

    • Author(s)
      Taura F, Iijima M, Yamanaka E, Takahashi H, Kenmoku H, Saeki H, Morimoto S, Asakawa Y, Kurosaki F, Morita H
    • Journal Title

      Front Plant Sci.

      Volume: 7 Pages: 1452-1452

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] エゾムラサキツツジ由来オルセリン酸生合成に関与する新規III型PKSの機能解析2017

    • Author(s)
      飯島未宇, 森田洋行, 黒崎文也, 田浦太志
    • Organizer
      第35回日本植物細胞分子生物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] エゾムラサキツツジのダウリクロメン酸生合成に関与するプレニル転移酵素2017

    • Author(s)
      佐伯春奈, 飯島未宇, 黒崎文也, 田浦太志
    • Organizer
      第35回日本植物細胞分子生物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 抗HIV成分を生産するエゾムラサキツツジの二次代謝2017

    • Author(s)
      田浦太志
    • Organizer
      第6回植物二次代謝フロンティア研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 生理活性植物メロテルペノイド生合成酵素の立体構造解明と機能的リデザイン2017

    • Author(s)
      田浦太志
    • Organizer
      新学術領域「生合成リデザイン」第3回公開シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Identification and characterization of daurichromenic acid synthase active in anti-HIV biosynthesis.2017

    • Author(s)
      Taura F
    • Organizer
      2nd TAA-Pharm Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] エゾムラサキツツジが生産する抗HIV天然物ダウリクロメン酸の生合成研究2017

    • Author(s)
      田浦太志
    • Organizer
      富山大学和漢医薬学総合研究所東西医薬学交流セミナー
    • Place of Presentation
      富山市
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 麹菌におけるDCA類縁体のコンビナトリアル生合成2016

    • Author(s)
      斉藤開, 三橋隆章, Chang Li, 松田侑大, 淡川孝義, 田浦太志, 阿部郁朗
    • Organizer
      第16回糸状菌分子生物学コンファレンス
    • Place of Presentation
      宇治市
    • Year and Date
      2016-11-17
    • Related Report
      2016 Research-status Report
  • [Presentation] 抗HIV天然物ダウリクロメン酸の生合成研究2016

    • Author(s)
      飯島未宇, 黒崎文也, 田浦太志
    • Organizer
      第21回天然薬物の開発と応用シンポジウム
    • Place of Presentation
      千葉市
    • Year and Date
      2016-10-27
    • Related Report
      2016 Research-status Report
  • [Presentation] エゾムラサキツツジが生産する抗HIV天然物ダウリクロメン酸の生合成研究2016

    • Author(s)
      飯島未宇, 田浦太志, 兼目裕充, 高橋宏暢, 豊田正夫, 李貞範, 黒崎文也, 浅川義範
    • Organizer
      第34回日本植物細胞分子生物学会大会
    • Place of Presentation
      長野県上田市
    • Year and Date
      2016-09-01
    • Related Report
      2016 Research-status Report
  • [Presentation] エゾムラサキツツジが生産する抗HIV天然物ダウリクロメン酸の生合成研究2016

    • Author(s)
      飯島未宇, 田浦太志, 兼目裕充, 高橋宏暢, 豊田正夫, 李貞範, 黒崎文也, 浅川義範
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      横浜市
    • Year and Date
      2016-03-26
    • Related Report
      2015 Research-status Report
  • [Presentation] エゾムラサキツツジが生産する抗HIV天然物ダウリクロメン酸の生合成研究2015

    • Author(s)
      飯島未宇, 田浦太志, 兼目裕充, 高橋宏暢, 豊田正夫, 李貞範, 黒崎文也, 浅川義範
    • Organizer
      2015年度日本農芸化学会中部・関西支部合同大会
    • Place of Presentation
      富山市
    • Year and Date
      2015-09-19
    • Related Report
      2015 Research-status Report
  • [Presentation] Molecular and biochemical characterization of daurichromenic acid synthase from Rhododendron dauricum.2015

    • Author(s)
      Iijima M, Taura F, Kenmoku H, Takahashi H, Toyota M, Lee J.-B, Kurosaki F, Asakawa Y
    • Organizer
      The Inaugural Symposium of the Phytochemical Society of Asia
    • Place of Presentation
      Tokushima, Japan.
    • Year and Date
      2015-08-30
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Molecular and biochemical characterization of polyketide synthase from Rhododendron dauricum.2015

    • Author(s)
      Taura F, Iijima M, Xie J, Takahashi H, Kenmoku H, Kurosaki F, Asakawa Y
    • Organizer
      The Inaugural Symposium of the Phytochemical Society of Asia
    • Place of Presentation
      Tokushima, Japan.
    • Year and Date
      2015-08-30
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Molecular and biochemical characterization of daurichromenic acid synthase from Rhododendron dauricum.2015

    • Author(s)
      Iijima M, Taura F, Kenmoku H, Takahashi H, Toyota M, Lee J.-B, Kurosaki F, Asakawa Y
    • Organizer
      Plant Biology 2015
    • Place of Presentation
      Minnesota, USA
    • Year and Date
      2015-07-26
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Book] Chapter 8 Cannabinoids: Biosynthesis and biotechnological applications. In Cannabis sativa L.- Botany and biotechnology2017

    • Author(s)
      Sirikantaramas S, Taura F
    • Total Pages
      474
    • Publisher
      Springer
    • ISBN
      9783319545639
    • Related Report
      2017 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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