| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Our group has recently cloned a gene cluster containing the biosynthetic genes for an antituberculosis antibiotic, D-cycloserine (D-CS) from D-CS-producing Streptomyces lavendulae. The aim of this study is to generate unnatural amino acids by using the enzymes in the D-CS-biosynthetic pathway. Based on the crystal structures of DcsD and DcsG, previously determined by us, mutational analyses were performed to identify the important residues for the catalytic activity and the substrate preference. In addition, crystal structure of DcsA was determined using the single anomalous dispersion method. Catalytic mechanism of DcsA was proposed based on the structural, mutational, and resonance Raman spectroscopic analyses.
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