| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
We have identified proteins that selectively bind a T3SS inhibitor, including its known ligand EF-Tu (elongation factor thermo unstable) and protein X encoded by gene X. For this target molecule, three strains were created: a gene X knockout strain, a vector complementary strain, and a gene X complementary strain. Inhibition of hemolysis and secretion of Esp (enterococcal surface protein) family proteins, which are components of the T3SS, were measured in these strains. As a result, hemolysis was found to be inactivated in the gene X knockout strain. This inactivation was partially recovered in the gene X complementary strain. Furthermore, secretion of Esp A, B, C, and D were decreased in the gene X knockout strain. The decrease in secretory levels was partially recovered in the gene X complementary strain. These results indicate that gene X is involved in T3SS expression and also regulates hemolytic activity.
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