| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We designed and synthesized a novel artificial 2’,4’-methylene bridged nucleic acid with a phenoxazine nucleobase (named BNAP). Oligonucleotide containing BNAP showed higher binding affinities toward complementary DNA and RNA. Thus, BNAP is a promising candidate for oligonucleotide therapeutics, especially for those requiring high duplex-forming ability.
On the other hand, we developed a novel artificial nucleic acid containing a 9-azaphenoxazine nucleobase (9-TAP), which behaves as a cytosine (C) analog as well as a thymine (T) analog. Oligonucleotides containing 9-TAP could form stable duplexes via metal-mediated base pairs with C and T, via AgI and HgII, respectively. Duplexes bearing the mismatched base pair 9-TAP-C were effectively stabilized in the presence of CuII. Thus, 9-TAP is expected to have applications in DNA nanotechnology via the formation of various nanostructures triggered by different metal ions.
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