• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of segment-specific cadmium transport mechanism in renal proximal tubules

Research Project

Project/Area Number 15K08054
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental and hygienic pharmacy
Research InstitutionTokushima Bunri University

Principal Investigator

Fujishiro Hitomi  徳島文理大学, 薬学部, 講師 (10389182)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords腎臓 / カドミウム / 輸送 / 近位尿細管 / 金属 / 毒性 / 腎障害 / 亜鉛輸送体 / バイオマーカー
Outline of Final Research Achievements

To analyze the proximal tubular dysfunction mechanism by cadmium (Cd), we utilized the mouse immortalized proximal tubule cells derived from S1, S2, S3 segments to examine the Cd transport and toxicity. There is no difference in the sensitivity to Cd in S1, S2, S3 cells, we found that the high uptake from the apical side of S3 cells where the expression of ZIP8, the transporter having a high affinity for Cd, is high. Moreover, the induction of Kim-1 which is a sensitive renal failure marker was observed in S1, S2, and S3 cells exposed to low concentrations of Cd. Since the purification of mouse MT protein has been made, it is expected to analyze the transport and toxicity mechanisms of Cd-MT in the future.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (24 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (17 results) (of which Int'l Joint Research: 2 results,  Invited: 5 results) Book (1 results) Remarks (2 results)

  • [Int'l Joint Research] University of Toronto/Laboratory Medicine & Pathobiology(Canada)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Concentration-dependent roles of DMT1 and ZIP14 in cadmium absorption in Caco-2 cells2017

    • Author(s)
      Fujishiro Hitomi、Hamao Satoko、Tanaka Rina、Kambe Taiho、Himeno Seiichiro
    • Journal Title

      The Journal of Toxicological Sciences

      Volume: 42 Issue: 5 Pages: 559-567

    • DOI

      10.2131/jts.42.559

    • NAID

      130006076713

    • ISSN
      0388-1350, 1880-3989
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Terminology of elemental speciation - An IUPAC perspective.2017

    • Author(s)
      Templeton, D.M., Fujishiro, H.
    • Journal Title

      Coord. Chem. Rev.

      Volume: 352 Pages: 424-431

    • DOI

      10.1016/j.ccr.2017.02.002

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Direct Comparison of Manganese Detoxification/Efflux Proteins and Molecular Characterization of ZnT10 Protein as a Manganese Transporter2016

    • Author(s)
      Nishito Y, Tsuji N, Fujishiro H, Takeda T, Yamazaki T, Teranishi F, Okazaki F, Matsunaga A, Tuschl K, Rao R, Kono S, Miyajima H, Narita H, Himeno S, Kambe T
    • Journal Title

      J. Biol. Chem.

      Volume: 291 Issue: 28 Pages: 14773-14787

    • DOI

      10.1074/jbc.m116.728014

    • NAID

      120006376011

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Lead accumulation and cytotoxicity in segment-specific kidney proximal tubule cells.2018

    • Author(s)
      〇Minerva Martinez- Alfaro, Hitomi Fujishiro, Seiichiro Himeno
    • Organizer
      日本薬学会138年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] DT40細胞における亜鉛輸送体ZIP8の変異によるマンガン輸送の変化2018

    • Author(s)
      〇宮崎寿和、藤代瞳、神戸大朋、姫野誠一郎
    • Organizer
      日本薬学会138年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 培養細胞を用いたカドミウムによる近位尿細管再吸収障害の評価2018

    • Author(s)
      〇岡奈々恵、藤代瞳、姫野誠一郎
    • Organizer
      日本薬学会138年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Cadmium-incduced autophagy through JNK and Ca2+ in rat mesangial cells.2017

    • Author(s)
      Hitomi Fujishiro and Douglas M. Templeton
    • Organizer
      日本薬学会 第137年会
    • Place of Presentation
      東北医科薬科大学・仙台
    • Year and Date
      2017-03-27
    • Related Report
      2016 Research-status Report
  • [Presentation] 腎臓の様々な部位由来細胞を用いたカドミウム毒性発現機構の解析2017

    • Author(s)
      〇藤代瞳、姫野誠一郎.
    • Organizer
      第44回日本毒性学会学術総会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 腎臓近位尿細管における有害金属および薬物の毒性と動態の解析2017

    • Author(s)
      〇藤代瞳
    • Organizer
      分子研研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 近位尿細管S1, S2, S3領域の活性酸素種感受性の差異2017

    • Author(s)
      ◯藤代瞳,山上りえ、姫野誠一郎.
    • Organizer
      フォーラム2017:衛生薬学・環境トキシコロジー
    • Related Report
      2017 Annual Research Report
  • [Presentation] カドミウムによる近位尿細管再吸収障害機構のin vitro解析2017

    • Author(s)
      ◯大寺信輝,山本葉月,藤代瞳, 姫野誠一郎
    • Organizer
      フォーラム2017:衛生薬学・環境トキシコロジー
    • Related Report
      2017 Annual Research Report
  • [Presentation] 腎臓近位尿細管における生体金属の動的な輸送機構と生体影響の解析2017

    • Author(s)
      〇藤代瞳、姫野誠一郎
    • Organizer
      生命化学系学会合同年次大会(ConBio2017)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] The roles of zinc transporters in the transport of cadmium and manganese in their target organs.2015

    • Author(s)
      H. Fujishiro and S. Himeno.
    • Organizer
      BMB2015(第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Investigation of cadmium transport in mouse kidney proximal tubule cells.2015

    • Author(s)
      H. Fujishiro and S. Himeno.
    • Organizer
      13th International Conference on the Biogeochemistry of Trace Elements
    • Place of Presentation
      福岡
    • Year and Date
      2015-07-13
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 腎臓近位尿細管部位特異的な細胞の毒性・輸送機構解析への応用.2015

    • Author(s)
      藤代瞳、伊澤美咲、杉本光、山本葉月、姫野誠一郎.
    • Organizer
      第42回日本毒性学会学術年会
    • Place of Presentation
      金沢
    • Year and Date
      2015-06-30
    • Related Report
      2015 Research-status Report
  • [Presentation] 重金属の毒性発現における金属輸送体の役割に関する研究.2015

    • Author(s)
      藤代瞳
    • Organizer
      第42回日本毒性学会学術年会
    • Place of Presentation
      金沢
    • Year and Date
      2015-06-30
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Cadmium transport and cytotoxicity in segment-specific kidney proximal tubule cells.2015

    • Author(s)
      H. Fujishiro, S. Hamao, and S. Himeno.
    • Organizer
      7th International Congress of ASIATOX (ASIATOX-VII)
    • Place of Presentation
      済州島(韓国)
    • Year and Date
      2015-06-26
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 腎臓近位尿細管部位特異的由来細胞を用いたカドミウム輸送機構の探索.2015

    • Author(s)
      藤代瞳
    • Organizer
      第10回トランスポーター研究会.
    • Place of Presentation
      東京
    • Year and Date
      2015-06-21
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Cytotoxicity and transport of cadmium and manganese in kidney proximal tubule segment-specific cells.2015

    • Author(s)
      M. Isawa, H. Fujishiro, and S. Himeno.
    • Organizer
      第25回金属の関与する生体関連反応シンポジウム
    • Place of Presentation
      長崎
    • Year and Date
      2015-05-30
    • Related Report
      2015 Research-status Report
  • [Presentation] Segment-specific induction of new biomarkers of nephrotoxicity in mouse kidney proximal tubule cells.2015

    • Author(s)
      S. Hamamo, H. Fujishiro, and S. Himeno.
    • Organizer
      第25回金属の関与する生体関連反応シンポジウム
    • Place of Presentation
      長崎
    • Year and Date
      2015-05-30
    • Related Report
      2015 Research-status Report
  • [Book] Metallomics - Recent Analytical Techniques and Applications2017

    • Author(s)
      Seiichiro Himeno and Hitomi Fujishiro
    • Total Pages
      779
    • Publisher
      Springer
    • Related Report
      2016 Research-status Report
  • [Remarks] 徳島文理大学薬学部

    • URL

      http://p.bunri-u.ac.jp/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 徳島文理大学薬学部衛生化学講座

    • URL

      http://p.bunri-u.ac.jp/lab10/index.html

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi