Drug resistance caused by intracellular pharmacokinetics

• Project/Area Number: 15K08066
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Gunma University
• Principal Investigator: YAMAMOTO KOUJIROU 群馬大学, 大学院医学系研究科, 教授 (70174787)
• Co-Investigator(Kenkyū-buntansha): 荒木 拓也 群馬大学, 大学院医学系研究科, 准教授 (00568248) ; 坡下 真大 名古屋市立大学, 大学院薬学研究科, 講師 (20613384) ; 永野 大輔 群馬大学, 大学院医学系研究科, 助教 (90738387)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 薬剤抵抗性 / 薬動力学 / 細胞内薬物濃度 / 細胞内薬物動態 / 薬力学 / 血中薬物濃度 / 細胞中薬物濃度 / PK-PD解析 / 薬物輸送担体 / ＰＫ－ＰＤ解析

Outline of Final Research Achievements

To establish a method of dosage adjustment for darunavir (DRV) based on pharmacokinetic theory, we analyzed the correlation between DRV levels in peripheral blood mononuclear cells (PBMCs) and plasma. An in vitro kinetic study using MOLT-4 cells was performed to assess the contribution of RTV to the intracellular accumulation of DRV. We found a poor correlation between intracellular DRV and plasma DRV levels in patients receiving HAART. The efflux rate of DRV from cells was slow; therefore, the concentration of DRV in PBMCs may reflect average exposure to the drug and clinical efficacy.
The contribution of carrier-mediated transport systems on the biliary elimination of gadoxetate was examined. The geometrical isomer with specific conformation corresponding to 22.6% of gadoxetate was eliminated into bile in rats via a carrier-mediated transport system no later than 30min after intravenous injection.

Research Products

• [Journal Article] Raltegravir is safely used with long-term viral suppression for HIV-infected patients on hemodialysis: a pharmacokinetic study. (2016)
  • Author(s): Kunio Yanagisawa, Daisuke Nagano, Yoshiyuki Ogawa, Hideki Uchiumia, Tetsuya Shigehara, Kazuhisa Saruki, Hiroshi Handa, Takuya Araki, Koujirou Yamamoto, Yoshihisa Nojima
  • Journal Title: ＡＩＤＳ Volume: 30 Issue: 6 Pages: 970-972
  • DOI: 10.1097/qad.0000000000001012
  • Peer Reviewed
• [Presentation] The influence of the genetic polymorphisms of efflux transporter on DRV levels in PBMC and plasma. (2017)
  • Author(s): Daisuke Nagano, Takuya Araki, Kunio Yanagisawa, Yoshiyuki Ogawa, Hideki Uchiumi, Koujirou Yamamoto
  • Organizer: The 13th Congress of the European association for clinical pharmacology and therapeutics (EACPT).
  • Int'l Joint Research
• [Presentation] The influence of the genetic polymorphisms of efflux transporter on DRV levels in PBMC and plasma. (2017)
  • Author(s): Daisuke Nagano, Takuya Araki, Kunio Yanagisawa, Yoshiyuki Ogawa, Hideki Uchiumi, Yoshihisa Nojima, Koujirou Yamamoto
  • Organizer: American Society of Clinical Pharmacology and Therapeutics Annual Meeting 2017
  • Int'l Joint Research
• [Remarks] 群馬大学大学院医学系研究科臨床薬理学分野
  • URL: http://clinpharmacol.dept.med.gunma-u.ac.jp/