| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
To evaluate the substrate specificity of organic cation transporter in the intestine, we examine uptake of 12 cationic compounds in human intestinal LS180 cells. As the results, the uptake was positively correlated with lipophilicity, and negatively correlated with polar surface area of drugs.
Orphan SLC22A15, 17, and 23 were expressed considerably in the intestine. We over-expresed these genes in renal LLC-PK1 or MDCK cells. However, the transport activity of quinidine was not increased in renal cells, suggesting that SLC22A15, 17, and 23 are not the organic cation transporter in the intestine.
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