Functional and structural analyses of organic cation transporter in the intestine.
| Project/Area Number |
15K08069
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | University of Toyama |
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Principal Investigator |
Hshimoto Yukiya 富山大学, 大学院医学薬学研究部(薬学), 教授 (90228429)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 小腸薬物吸収 / キニジン / 脂溶性カチオン / トランスポーター / 有機カチオン / キニッジン / SCL22A17 |
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| Outline of Final Research Achievements |
To evaluate the substrate specificity of organic cation transporter in the intestine, we examine uptake of 12 cationic compounds in human intestinal LS180 cells. As the results, the uptake was positively correlated with lipophilicity, and negatively correlated with polar surface area of drugs.
Orphan SLC22A15, 17, and 23 were expressed considerably in the intestine. We over-expresed these genes in renal LLC-PK1 or MDCK cells. However, the transport activity of quinidine was not increased in renal cells, suggesting that SLC22A15, 17, and 23 are not the organic cation transporter in the intestine.
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Report
(4 results)
Research Products
(5 results)
