The effect of granulocyte macrophage-colony stimulating factor on histogenesis of the brain of mouse embryos
| Project/Area Number |
15K08135
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Shimane University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
大谷 浩 島根大学, 医学部, 教授 (20160533)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 脳・神経 / 発生・分化 / GM-CSF / サイトカイン / ＧＭ－ＣＳＦ |
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| Outline of Final Research Achievements |
Granulocyte macrophage-colony stimulating factor (GM-CSF) is a multifunctional cytokine. In this study, we examined the effect of exogenously added GM-CSF in the brain development of mouse embryos. We injected GM-CSF into the lateral ventricle of embryos on embryonic day (E) 13 and let them develop until E15 by exo utero development system. From histological analysis, a part of luminal surface of ganglionic eminence became irregular and formed ‘bulge’ by disarrangement of the neuroepithelial cells in the GM-CSF-injected brain. These irregular cells were the neuronal precursor cells which lost proliferative activity and the apico-basal cell polarity. These results suggest that excess GM-CSF in cerebrospinal fluid may affect proliferation of neuroepithelial cells, migration of post-mitotic neuronal precursor cells, and disturb the apico-basal cell polarity at the luminal side of the ganglionic eminence of mouse embryonic brain.
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Report
(4 results)
Research Products
(22 results)
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[Presentation] Interkinetic nuclear migration in the developing esophageal, tracheal, and intestinal epithelia.2017
Author(s)
Otani H, Nitta T, Ogawa N, Kaneda R, Saeki Y, Rafiq AM, Jahan E,Regassa DG,, Furuya M, Matsumoto A, Udagawa J, Hatta T
Organizer
第122回日本解剖学会全国学術集会
Place of Presentation
長崎市、長崎大学坂本キャンパス
Year and Date
2017-03-28
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[Presentation] Interkinetic nuclear migration in the developing endoderm- and mesoderm-origin epithelial tubular structures2016
Author(s)
Otani H, Motoya T, Ogawa N, Nitta T, Rafiq AM, Jahan E, Kaneda R, Furuya M, Matsumoto A, Udagawa J, Hatta T
Organizer
第121回日本解剖学会全国学術集会
Place of Presentation
ビックパレット福島（郡山市）
Year and Date
2016-03-28
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