| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Granulocyte macrophage-colony stimulating factor (GM-CSF) is a multifunctional cytokine. In this study, we examined the effect of exogenously added GM-CSF in the brain development of mouse embryos. We injected GM-CSF into the lateral ventricle of embryos on embryonic day (E) 13 and let them develop until E15 by exo utero development system. From histological analysis, a part of luminal surface of ganglionic eminence became irregular and formed ‘bulge’ by disarrangement of the neuroepithelial cells in the GM-CSF-injected brain. These irregular cells were the neuronal precursor cells which lost proliferative activity and the apico-basal cell polarity. These results suggest that excess GM-CSF in cerebrospinal fluid may affect proliferation of neuroepithelial cells, migration of post-mitotic neuronal precursor cells, and disturb the apico-basal cell polarity at the luminal side of the ganglionic eminence of mouse embryonic brain.
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