| Project/Area Number |
15K08146
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Tohoku Fukushi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
尾形 雅君 東北医科薬科大学, 薬学部, 講師 (50311907)
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| Project Period (FY) |
2015-10-21 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | マウス / 小腸 / 上皮細胞 / γδ-IELs / TNFα / DNA断片化 / パーフォリン / グランザイム / マクロファージ / 粘膜固有層 / IEL / DNA修復 / Granzyme B / Perforin |
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| Outline of Final Research Achievements |
γδ-IELs appear to readily and rapidly respond, though at only one time, to various stimulations. They could be recognized as one-shot responders and disposable cells of the defense system present in the front line intestinal epithelium. Activated γδ-IELs not only induced DNA fragmentation in villus epithelial cells (IECs); it induced identical DNA fragmentation in the γδ-IELs themselves (the autocrine DNA fragmentation). The DNA fragmentation as well as the DNA repair thereafter should thus be interpreted in a more generalized phenomenon. The transient diarrhea due to the detachment of IECs induced by activated γδ-IELs was shown to be caused by TNFα derived from macrophages located in the lamina propria, suggesting the involvement of the γδ-IELs in the elimination of IECs in a defense system, for example, when IECs are infected with pathogenic viruses.
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