| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Outline of Final Research Achievements |
Intraventricular injection of lipophilic dye enabled the labeling of the ependymal cells/radial glial cells in the Xenopus laevis, and new neurons are considered to be derived from these cells. For establishing the system for the spatiotemporal expression of the exogenous genes in amphibians, we compared the efficacy of gene recombination systems and found that the efficacy of the expression of the exogenous gene in the Flp/FRT system is superior to that in the Cre/loxP system at a genome level, however, that in the latter one is almost similar to that in the former one at a level of the activity of fluorescent dyes.
|
