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Functional analysis of gastrointestinal interstitial cells using genetic labeling technique

Research Project

Project/Area Number 15K08150
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General anatomy (including histology/embryology)
Research InstitutionUniversity of Fukui

Principal Investigator

IINO SATOSHI  福井大学, 学術研究院医学系部門, 教授 (40242854)

Co-Investigator(Kenkyū-buntansha) 堀口 和秀  福井大学, 学術研究院医学系部門, 准教授 (20377451)
橋本 隆  福井大学, 学術研究院医学系部門, 特命助教 (60712891)
Co-Investigator(Renkei-kenkyūsha) HORIGUCHI SATOMI  福井大学, 学術研究院医学系部門, 特別研究員 (00595283)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords間質細胞 / 線維芽細胞 / カハール介在細胞 / PDGF受容体α / c-Kit / ヘッジホッグ / 消化管
Outline of Final Research Achievements

We observed gastrointestinal interstitial cells such as interstitial cells of Cajal (ICC) and fibroblasts (fibroblast-like cells) using genetic labeling mice. We showed that fibroblasts in the musculature expressed c-Kit ligand SCF (stem cell factor) that is essential for ICC development. Using lacZ reporter mice, we showed that ICC and fibroblasts expressed zinc finger protein Gli1 and Gli2 known as glioma-associated oncogene. These proteins are transcription factors mediating the hedgehog pathway and regulate gastrointestinal tract development. Our findings suggest that ICC and fibroblasts are regulated by hedgehog signaling in development and maintenance of gastrointestinal tract.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (26 results)

All 2018 2017 2016 2015

All Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (20 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] The anti-inflammatory pathway regulated via nicotinic acetylcholine receptors in rat intestinal mesothelial cells2017

    • Author(s)
      Taiki Mihara, Wataru Otsubo, Kazuhide Horiguchi, Shoma MikawaNoriyuki Kaji, Satoshi Iino, Hiroshi Ozaki and Masatoshi Hori
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 79 Issue: 11 Pages: 1795-1802

    • DOI

      10.1292/jvms.17-0304

    • NAID

      130006199240

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nitric oxide-induced oxidative stress impairs pacemaker function of murine interstitial cells of Cajal during inflammation2016

    • Author(s)
      Kaji N, Horiguchi K, Iino S, Nakayama S, Ohwada T, Otani Y, Firman, Murata T, Sanders KM, Ozaki H, Hori M
    • Journal Title

      Pharmacological Research

      Volume: 111 Pages: 838-848

    • DOI

      10.1016/j.phrs.2016.07.030

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Epidermal growth factor is a critical regulator of the cytokine IL-33 in intestinal epithelial cells.2016

    • Author(s)
      Islam MS, Horiguchi K, Iino S, Kaji N, Mikawa S, Hori M, Ozaki H.
    • Journal Title

      British Journal of Pharmacology

      Volume: 173 Issue: 16 Pages: 2532-2542

    • DOI

      10.1111/bph.13535

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Therapeutic action of 5-HT3 receptor antagonists targeting peritoneal macrophages in postoperative ileus.2015

    • Author(s)
      Maehara T; Matsumoto K; Horiguchi K; Kondo M; Iino S; Horie S; Murata T; Tsubone H; Shimada S; Ozaki H; Hori M.
    • Journal Title

      Br J Pharmacol

      Volume: 172(4) Issue: 4 Pages: 1136-1147

    • DOI

      10.1111/bph.13006

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Utility of postmortem imaging system for anatomical education in skull base surgery.2015

    • Author(s)
      Kodera T, Arishima H, Kitai R, Kikuta K, Iino S, Noriki S, Naiki H.
    • Journal Title

      Neurosurg Rev.

      Volume: 38 Issue: 1 Pages: 165-170

    • DOI

      10.1007/s10143-014-0574-2

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Convergence of Lemniscal and Local Excitatory Inputs on Large GABAergic Tectothalamic Neurons.2015

    • Author(s)
      Ito T, Hioki H, Sohn J, Okamoto S, Kaneko T, Iino S, Oliver DL.
    • Journal Title

      J Comp Neurol

      Volume: In Press Issue: 15 Pages: 2277-96

    • DOI

      10.1002/cne.23789

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 成体マウス消化管における転写因子Gli1とGli2の局在解析2018

    • Author(s)
      橋本 隆、堀口 和秀、堀口 里美、飯野 哲
    • Organizer
      第123回 日本解剖学会総会・全国学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 消化管筋層におけるc-KIT受容体型チロシンキナーゼ発現による細胞系譜制御の解明2018

    • Author(s)
      堀口 里美、堀口 和秀、飯野 哲
    • Organizer
      第123回 日本解剖学会総会・全国学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] TNBS腸炎モデルマウス筋層の炎症回復過程におけるカハール介在細胞増殖2018

    • Author(s)
      堀口 和秀、堀口 里美、橋本 隆、飯野 哲
    • Organizer
      第123回 日本解剖学会総会・全国学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] TNBS腸炎モデルマウスにおける消化管筋層の傷害と回復過程2017

    • Author(s)
      堀口和秀、河原真代、杉本京平、堀口里美、橋本隆、飯野哲
    • Organizer
      第122回日本解剖学会総会全国学術集会
    • Place of Presentation
      長崎大学(長崎市・長崎県)
    • Year and Date
      2017-03-30
    • Related Report
      2016 Research-status Report
  • [Presentation] 胎生期マウス消化管筋層のc-KIT受容体型チロシンキナーゼの発現解析2017

    • Author(s)
      堀口里美、堀口和秀、飯野哲
    • Organizer
      第122回日本解剖学会総会全国学術集会
    • Place of Presentation
      長崎大学(長崎市・長崎県)
    • Year and Date
      2017-03-30
    • Related Report
      2016 Research-status Report
  • [Presentation] マウス消化管における転写因子Gli1の局在解析2017

    • Author(s)
      橋本隆、堀口和秀、堀口里美、飯野哲
    • Organizer
      第122回 日本解剖学会総会全国学術集会
    • Place of Presentation
      長崎大学(長崎市・長崎県)
    • Year and Date
      2017-03-30
    • Related Report
      2016 Research-status Report
  • [Presentation] c-Kitシグナル異常を持つマウスにおける小腸カハール介在細胞の発生2017

    • Author(s)
      飯野 哲、堀口里美、堀口和秀、橋本隆
    • Organizer
      日本顕微鏡学会 第73回学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] TNBS腸炎モデルマウスにおける消化管筋層の傷害と回復過程2017

    • Author(s)
      飯野哲、堀口和秀、堀口里美、橋本隆、川原真代、杉本京平
    • Organizer
      第59回日本平滑筋学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 成体マウス消化管における転写因子Gli1の局在解析2017

    • Author(s)
      橋本 隆、堀口 和秀、堀口 里美、飯野 哲
    • Organizer
      第59回日本平滑筋学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] TNBS腸炎モデルマウスを用いた結腸筋層の傷害と回復過程研究2017

    • Author(s)
      飯野哲、堀口和秀、堀口里美、橋本隆、川原真代、杉本京平
    • Organizer
      第49回日本臨床分子形態学会総会・学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 成体マウス消化管におけるGli1陽性細胞の同定2017

    • Author(s)
      橋本 隆、堀口 和秀、堀口 里美、飯野 哲
    • Organizer
      第58回日本組織細胞化学会総会・学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] PTSDモデルストレス負荷に伴う、ラット脳内CRHの発現異常2016

    • Author(s)
      橋本隆、松田賢一、吉井崇喜、河田光博、飯野哲
    • Organizer
      第121回 日本解剖学会総会全国学術集会
    • Place of Presentation
      ビックパレットふくしま(福島県)
    • Year and Date
      2016-03-30
    • Related Report
      2015 Research-status Report
  • [Presentation] TNBs腸炎マウス筋層におけるカハール介在細胞回復過程の組織学的検討2016

    • Author(s)
      堀口里美、堀口和秀、橋本隆、飯野哲
    • Organizer
      第121回 日本解剖学会総会全国学術集会
    • Place of Presentation
      ビックパレットふくしま(福島県)
    • Year and Date
      2016-03-30
    • Related Report
      2015 Research-status Report
  • [Presentation] PTSDモデルラットの脳内におけるCRHの発現変化2015

    • Author(s)
      橋本隆、松田賢一、吉井崇喜、河田光博、飯野 哲
    • Organizer
      日本解剖学会第75回中部支部学術集会
    • Place of Presentation
      福井大学(福井県)
    • Year and Date
      2015-10-03
    • Related Report
      2015 Research-status Report
  • [Presentation] 堀口里美,堀口和秀,飯野哲2015

    • Author(s)
      マウス消化管筋層のカハール介在細胞と線維芽細胞におけるムスカリン性アセチルコリン受容体の発現解析
    • Organizer
      日本解剖学会第75回中部支部学術集会
    • Place of Presentation
      福井大学(福井県)
    • Year and Date
      2015-10-03
    • Related Report
      2015 Research-status Report
  • [Presentation] 消化管におけるカハール介在細胞とその類縁細胞2015

    • Author(s)
      飯野哲
    • Organizer
      第47回日本臨床分子形態学会総会・学術集会
    • Place of Presentation
      長崎大学(長崎県)
    • Year and Date
      2015-09-18
    • Related Report
      2015 Research-status Report
  • [Presentation] 消化管間質を構成する細胞群の形態学的特性2015

    • Author(s)
      飯野 哲、堀口 和秀
    • Organizer
      第57回日本平滑筋学会総会
    • Place of Presentation
      山口大学小串キャンパス(山口県)
    • Year and Date
      2015-08-28
    • Related Report
      2015 Research-status Report
  • [Presentation] 術後イレウスにおけるInterstitial cells of Cajal の病態変化特性2015

    • Author(s)
      梶 典幸、中山 晋介、堀口 和秀、飯野 哲、尾崎 博、堀 正敏
    • Organizer
      第57回日本平滑筋学会総会
    • Place of Presentation
      山口大学小串キャンバス(山口県)
    • Year and Date
      2015-08-28
    • Related Report
      2015 Research-status Report
  • [Presentation] PTSDモデルラットの脳内におけるストレス関連因子の発現変化2015

    • Author(s)
      橋本 隆、松田 賢一、飯野 哲、河田 光博
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2015-07-28
    • Related Report
      2015 Research-status Report
  • [Presentation] The expression of small conductance calcium-activated potassium (SK3)channel is specifically concentrated at interstitial cells of the spaces between detrusor smooth muscle cells in the fuinea pig urinary bladder.2015

    • Author(s)
      T Hayashi, M Takeya, K Uemura, T Kanazawa, S Iino, T Igawa, K Nakamura
    • Organizer
      Symposium on Multicellular Inputs Regulating Muscle Excitability
    • Place of Presentation
      Tahoe(アメリカ)
    • Year and Date
      2015-07-14
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2019-03-29  

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