Dissection of the regulatory mechanisms of male germ stem cell by DNA methylation mediated through transcription factors
| Project/Area Number |
15K08156
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Yokohama City University |
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Principal Investigator |
Ohbo Kazuyuki 横浜市立大学, 医学研究科, 教授 (70250751)
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| Research Collaborator |
Matsumoto Naomichi
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 生殖細胞 / 幹細胞 / エピゲノム / 細胞・組織 / 解剖学 / エピジェネティクス / 生殖 / 分化 |
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| Outline of Final Research Achievements |
We previously identified the epigenetic checkpoint where various epigenetic factors and modifications are changed during male germ stem cell differentiation. In this study, we identified the seed enhancers that showed drastic changes during the male germ stem cell differentiation. We also observed chromosomal localization of the genomes where DNA methylation levels were up-regulated during the stem cell differentiation.
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| Academic Significance and Societal Importance of the Research Achievements |
精子を、個体の一生涯作り続ける元となる細胞は、精子幹細胞と呼ばれている。精子幹細胞が分裂する際の制御は、サイトカインなど、シグナル分子を介した研究が盛んである。しかし、本研究では、まったく異なる視点となるエピジェネティクスの仕組みから、精子幹細胞を俯瞰した。特に、既に取得しているDNAメチル化模様の結果に、新たにEnhancerやSuper enhancerのヒストン修飾であるH3K4me1, H3K27acの修飾部位をゲノムワイドに決定する共に、幹細胞から分化開始時に大きく変化する部位が核内でどのように位置を変えるのか観察した。
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Report
(5 results)
Research Products
(20 results)
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[Journal Article] Kmt2b conveys monovalent and bivalent H3K4me3 in spermatogonial stem cells at germline and embryonic promoters2018
Author(s)
Tomizawa S, Kobayashi Y, Shirakawa T, Watanabe K, Mizoguchi K, Hoshi I, Nakajima K, Nakabayashi J, Singh S, Dahl A, Alexopoulou D, Seki M, Suzuki Y, Royo H, Peters6, A. H.F.M, Anastassiadis K, Stewart A.F. and Ohbo K
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Journal Title
Development
Volume: 145
Issue: 23
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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