Histological and functional analysis in the subregion of mammalian circadian center

• Project/Area Number: 15K08163
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Kindai University
• Principal Investigator: KOINUMA Satoshi 近畿大学, 医学部, 講師 (10340770)
• Co-Investigator(Kenkyū-buntansha): 長野 護 近畿大学, 医学部, 准教授 (80155960)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 視交叉上核 / 概日リズム / 位相波 / Per2 / 短周期領域 / 長周期領域

Outline of Final Research Achievements

Mammals have an intrinsic biological clock that shows a stable period of about 24 hours. Underlying this function is the suprachiasmatic nucleus (SCN) in the hypothalamus. We have elucidated that there are cell groups that oscillate rhythms shorter than this period in the dorso-medial region of the SCN (DM-SCN). And we have focused on a phase wave (the expression of the clock gene propagates from medial to lateral SCN) originating from DM-SCN. The presence of spreading of phase wave in the rostro-caudal axis direction in addition to the phase waves towards the lateral sides which was known so far was found by histochemical analysis using the mouse SCN. This result will deepen the basic understanding of the physiologically unknown function of the phase wave.

Research Products

• [Journal Article] CLOCKΔ19 mutation modifies the manner of synchrony among oscillation neurons in the suprachiasmatic nucleus (2018)
  • Author(s): Sujino Mitsugu、Asakawa Takeshi、Nagano Mamoru、Koinuma Satoshi、Masumoto Koh-Hei、Shigeyoshi Yasufumi
  • Journal Title: Sci Rep Volume: 8 Issue: 1 Pages: 854-854
  • DOI: 10.1038/s41598-018-19224-1
  • Peer Reviewed / Open Access
• [Journal Article] Transition of phase response properties and singularity in the circadian limit cycle of cultured cells (2017)
  • Author(s): Koinuma Satoshi、Kori Hiroshi、Tokuda Isao T.、Yagita Kazuhiro、Shigeyoshi Yasufumi
  • Journal Title: PLOS ONE Volume: 12 Issue: 7 Pages: 1-16
  • DOI: 10.1371/journal.pone.0181223
  • Peer Reviewed / Open Access
• [Journal Article] Effects of aging on basement membrane of the soleus muscle during recovery following disuse atrophy in rats (2017)
  • Author(s): Kanazawa Yuji、Ikegami Keisuke、Sujino Mitsugu、Koinuma Satoshi、Nagano Mamoru、Oi Yuki、Onishi Tomoya、Sugiyo Shinichi、Takeda Isao、Kaji Hiroshi、Shigeyoshi Yasufumi
  • Journal Title: Experimental Gerontology Volume: 98 Pages: 153-161
  • DOI: 10.1016/j.exger.2017.08.014
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Temperature?amplitude coupling for stable biological rhythms at different temperatures (2017)
  • Author(s): Kurosawa Gen、Fujioka Atsuko、Koinuma Satoshi、Mochizuki Atsushi、Shigeyoshi Yasufumi
  • Journal Title: PLOS Computational Biology Volume: 13 Issue: 6 Pages: e1005501-e1005501
  • DOI: 10.1371/journal.pcbi.1005501
  • Peer Reviewed / Open Access
• [Journal Article] Temperature-Amplitude Coupling for Stable Biological Rhythms at Different Temperatures (2017)
  • Author(s): Gen Kurosawa, Atsuko Fujioka, Satoshi Koinuma, Atsushi Mochizuki, Yasufumi Shigeyoshi
  • Journal Title: PLOS Computational Biology Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Reduction of translation rate stabilizes circadian rhythm and reduces the magnitude of phase shift (2015)
  • Author(s): 中嶋正人、鯉沼聡、重吉康史
  • Journal Title: Biochem Biophys Res Commun Volume: 464 Issue: 1 Pages: 354-359
  • DOI: 10.1016/j.bbrc.2015.06.158
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 老化がラット骨格筋の基底版構築に与える影響 (2018)
  • Author(s): 金澤佑治、池上啓介、筋野貢、鯉沼聡、長野護、杉生真一、武田功、梶博史、重吉康史
  • Organizer: 第123回日本解剖学会・全国学術集会
• [Presentation] 後肢非荷重と再荷重が老化したヒラメ筋に与える影響 (2017)
  • Author(s): 金澤佑治,、筋野貢、鯉沼聡、長野護、池上啓介、大井優紀、大西智也、杉生真一、武田功、梶博史、重吉康史
  • Organizer: 第122回 日本解剖学会総会・全国学術集会
  • Place of Presentation: 長崎大学坂本キャンパス（長崎市）
  • Year and Date: 2017-03-28
• [Presentation] 後肢免荷と再荷重が老化した骨格筋の細胞外マトリクスに与える影響 (2016)
  • Author(s): 金澤佑治,、筋野貢、鯉沼聡、長野護、池上啓介、大井優紀、大西智也、杉生真一、武田功、梶博史、重吉康史
  • Organizer: 第92回 日本解剖学会近畿支部学術集会
  • Place of Presentation: 近畿大学（東大阪市）
• [Presentation] 概日リズムの頑健性と安定性の制御 (2015)
  • Author(s): 中嶋正人、鯉沼聡、重吉康史
  • Organizer: 第22回時間生物学会学術大会
  • Place of Presentation: 東京、東京大学
  • Year and Date: 2015-11-21
• [Presentation] マウス培養視交叉上核における領域特異的周期差の探索 (2015)
  • Author(s): 筋野貢、鯉沼聡、重吉康史
  • Organizer: 第22回時間生物学会学術大会
  • Place of Presentation: 東京、東京大学
  • Year and Date: 2015-11-21
• [Presentation] Effect of sublethal dose of inhibitors of some molecular processes in the circadian negative feedback loop on stability of circadian rhythm (2015)
  • Author(s): Masato Nakajima, Satoshi Koinuma, Yasufumi Shigeyoshi
  • Organizer: The European Biological Rhythms Society (EBRS)/World Congress of Chronobiology (WCC) meeting
  • Place of Presentation: Manchester, UK
  • Year and Date: 2015-08-02