Correlative Structural Analysis of Microtubule Dynamics Regulation by CRMP2
| Project/Area Number |
15K08168
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Kobe University (2017)
Institute of Physical and Chemical Research (2015-2016) |
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Principal Investigator |
Nitta Ryo 神戸大学, 医学研究科, 教授 (40345038)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 微小管 / CRMP2 / Kinesin / 軸索誘導 / 軸索反発 / X線結晶構造解析 / X線小角散乱 / クライオ電子顕微鏡 / 微小管ダイナミクス / X線結晶解析 |
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| Outline of Final Research Achievements |
The aim of this project is to elucidate the molecular and structural mechanisms of CRMP2-regulated axon induction/repulsion. During the neuronal cell development, CRMP2 localizes the tips of the future axon in which CRMP2 forms the hetero-trimer with GTP-tubulin to add tubulins to the plus-ends of microtubules. This effectively induces the axonal microtubule formation, and thus the axon is effectively elongated. On the other hand, phosphorylation of CRMP2 decreases the affinity between CRMP2 and microtubule. It is driven by the small conformational changes at the C-terminal tail of CRMP2 with shifting the surface charges, which not only alter the interactions within the CRMP2 tetramer but also alter the interactions with GTP-tubulin. Consequently, phosphorylated CRMP2 fails to form a hetero-trimer with GTP-tubulin, thus losing the ability to establish and maintain the axonal microtubules.
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Report
(4 results)
Research Products
(24 results)
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[Presentation] Motility and Microtubule Depolymerization Mechanisms of the Kinesin-8 motor, KIF19A.2016
Author(s)
Wang, D, Nitta, R, Morikawa, M, Yajima H, Inoue, S, Shigematsu, H, Kikkawa, M, Hirokawa N
Organizer
米国細胞生物学会年会
Place of Presentation
San Francisco（アメリカ合衆国）
Year and Date
2016-12-06
Related Report
Int'l Joint Research
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