Proton leak mechanisms in acidic environments
| Project/Area Number |
15K08184
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Osaka City University |
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Principal Investigator |
Kuno Miyuki 大阪市立大学, 大学院医学研究科, 准教授 (00145773)
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| Co-Investigator(Kenkyū-buntansha) |
酒井 啓 大阪市立大学, 大学院医学研究科, 研究員(移行) (90382192)
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| Co-Investigator(Renkei-kenkyūsha) |
NOTOMI Takuya 大阪歯科大学, 歯学研究科, 講師 (70542249)
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| Research Collaborator |
LI Gunagshuai , 大学院学生
KAIJU Taro , 学部学生
MORIURA Yoshie , 研究補佐
HINO Yoshiko , 研究補佐
KAWAWAKI Junko , 技術職員
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 生体膜 / チャネル / トランスポータ / 能動輸送 / プロトン |
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| Outline of Final Research Achievements |
There are many local acidic spots in both intracellular and extracellular spaces. The pH level, intimately related to each specific function, is maintained by balancing acidification by proton (H+) pumps and its elimination (alkalization) by H+ leak mechanisms. The molecular entity and the property of the H+ leak remain to be clarified. We demonstrated the acid-inducible H+ leak in the plasma membrane of osteoclasts which resorb bone tissues by secreting massive amounts of acids on the bone surface. We also revealed that inorganic phosphates stimulated voltage-gated H+ channels and that the pH in single phagosomes often oscillates, which might contribute to H+ leakages in the specific occasions. These findings help us to clarify physiological/pathological mechanisms of regulation of acidic environments by H+ leak mechanisms.
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Report
(4 results)
Research Products
(38 results)
