The investigation of CAPS2-dependent secretion of oxytocin

• Project/Area Number: 15K08192
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: Tokyo University of Pharmacy and Life Science (2016-2017); Tokyo University of Science (2015)
• Principal Investigator: SHINODA YO 東京薬科大学, 薬学部, 講師 (80403096)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: CAPS / oxytocin / オキシトシン / CAPS2 / CAPS1

Outline of Final Research Achievements

The aim of this study is to investigate whether CAPS protein contributes oxytocin (OXT) secretion. In this projects’ period, we observed that 1: OXT level was significantly reduced in blood and increased in pituitary in Caps2 KO mice. 2: Similar tendency were observed in Caps2 dex3 mice. 3: The expression and subsequent transportation of OXT into axon terminal in Caps2 dex3 mice were identical to WT. 4: OXT neurons were heterogeneous population, some of them expressed both CAPS1 and 2 but another expressed CAPS1 or 2. 5: OXT neuron specific Caps1 cKO mice showed significant increasing of sociality. Taken together, these result suggest that both CAPS1 and CAPS2 are possibly involved in OXT secretion.

Research Products

• [Journal Article] CAPS2 deficiency affects environmental enrichment-induced adult neurogenesis and differentiation/survival of newborn neurons in the hippocampal dentate gyrus (2017)
  • Author(s): Yagishita Kaori、Suzuki Ritsuko、Mizuno Shota、Katoh-Semba Ritsuko、Sadakata Tetsushi、Sano Yoshitake、Furuichi Teiichi、Shinoda Yo
  • Journal Title: Neuroscience Letters Volume: 661 Pages: 121-125
  • DOI: 10.1016/j.neulet.2017.09.047
  • Peer Reviewed
• [Journal Article] The brain-specific RasGEF very-KIND is required for normal dendritic growth in cerebellar granule cells and proper motor coordination (2017)
  • Author(s): Hayashi K, Furuya A, Sakamaki Y, Akagi T, Shinoda Y, Sadakata T, Hashikawa T, Shimizu K, Minami H, Sano Y, Nakayama M, and Furuichi T.
  • Journal Title: PLoS ONE Volume: 12 Issue: 3 Pages: e0173175-e0173175
  • DOI: 10.1371/journal.pone.0173175
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Comparative photodynamic therapy cytotoxicity of mannose-conjugated chlorin and talaporfin sodium in cultured human and rat cells (2017)
  • Author(s): Shinoda Y, Takahashi T, Akimoto J, Ichikawa M, Yamazaki H, Narumi A, Yano S and *Fujiwara Y.
  • Journal Title: The Journal of Toxicological Sciences Volume: 42 Issue: 1 Pages: 111-119
  • DOI: 10.2131/jts.42.111
  • NAID: 130005286650
  • ISSN: 0388-1350, 1880-3989
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Analysis of gene expression in Ca2+-dependent activator protein for secretion 2 (Cadps2) knockout cerebellum using GeneChip and KEGG pathways (2016)
  • Author(s): Sadakata T, Shinoda Y, Ishizaki Y, Furuichi T.
  • Journal Title: Neuroscience Letters Volume: 639 Pages: 88-93
  • DOI: 10.1016/j.neulet.2016.12.068
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mammalian-specific central myelin protein Opalin is redundant for normal myelination: structural and behavioral assessments (2016)
  • Author(s): Yoshikawa, F., Sato, Y., Tohyama, K., Akagi, T., Furuse, T., Sadakata, T., Tanaka, M., Shinoda, Y., Hashikawa, T., Itohara, S., Sano, Y., Ghandour, SM., Wakana, S., and Furuichi, T.
  • Journal Title: PLoS ONE Volume: 11 Issue: 11 Pages: e0166732-e0166732
  • DOI: 10.1371/journal.pone.0166732
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Galacto-N-biose is neuroprotective against glutamate-induced excitotoxicity in vitro. (2016)
  • Author(s): Shinoda, Y., Nakajima, Y., Iguchi, H., Tatsumi, S., Kitaoka, M., Nakajima, M., Takahashi, T., Fujiwara, M., and Furuichi, T.
  • Journal Title: Eur. J. Pharmacol. Volume: 791 Pages: 711-717
  • DOI: 10.1016/j.ejphar.2016.10.010
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] CAPS1 stabilizes the state of readily releasable synaptic vesicles to fusion competence at CA3-CA1 synapses in adult hippocampus (2016)
  • Author(s): 6.Shinoda Y, Ishii C, Fukazawa Y, Sadakata T, Ishii Y, Sano Y, Iwasato T, Itohara S, Furuichi T
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 31540-31540
  • DOI: 10.1038/srep31540
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Low doses of the mycotoxin citrinin protect cortical neurons against glutamate-induced excitotoxicity (2016)
  • Author(s): Nakajima Y, Iguchi H, Kamisuki S, Sugawara F, Furuichi T and Shinoda Y
  • Journal Title: The Journal of Toxicological Sciences Volume: 41 Issue: 2 Pages: 311-319
  • DOI: 10.2131/jts.41.311
  • NAID: 130005132670
  • ISSN: 0388-1350, 1880-3989
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Interaction of Ca2+-dependent activator protein for secretion 1 (CAPS1) with septin family proteins in mouse brain (2016)
  • Author(s): Hosono M, Shinoda Y, Hirano T, Ishizaki Y, Furuichi T and Sadakata T
  • Journal Title: Neurosci. Lett. Volume: 617 Pages: 232-235
  • DOI: 10.1016/j.neulet.2016.02.035
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] A rubber tail task in CAPS2 KO mice: second report. (2016)
  • Author(s): Makoto Wada, Masakazu Ide, Takeshi Atsumi, Kaori Yagishita, Momoko Katakai, Yo Shinoda, Teiichi Furuichi and Kenji Kansaku.
  • Organizer: 76th Annual Meeting of the Japanese Society for Animal Psychology
  • Place of Presentation: Hokkaido Univ., Sapporo, Japan
  • Year and Date: 2016-11-23
  • Int'l Joint Research
• [Presentation] CAPS1 stabilizes docking state of SVs in hippocampal CA3-CA1 synapses (2016)
  • Author(s): Yo Shinoda, Chiaki Ishii, Yugo Fukazawa and Teiichi Furuichi
  • Organizer: The 93th Annual Meeting of the Physiological Society of Japan 2016
  • Place of Presentation: 札幌コンベンションセンター（北海道）
  • Year and Date: 2016-03-22
  • Int'l Joint Research
• [Presentation] A rubber tail task in Ca2+-dependent activator protein for secretion (CAPS) 2 knockout mice. (2016)
  • Author(s): M Wada, M Ide, T. Atsumi, K. Yagishita, M. Katakai, Y. Shinoda, T. Furuichi. K. Kansaku.
  • Organizer: Neuroscience 2016.
  • Place of Presentation: San Diego convention center, CA, US
  • Int'l Joint Research
• [Presentation] A deficiency of Ca2+-dependent activator protein for secretion 1 affects hippocampal long-term potentiation. (2016)
  • Author(s): Yuki Ishii, Chiaki Ishii, Yo Shinoda, Yoshitake Sano and Teiichi Furuichi.
  • Organizer: Neuroscience 2016
  • Place of Presentation: San Diego convention center, CA, US
  • Int'l Joint Research
• [Presentation] CAPS1 stabilizes docking state of SVs in hippocampal CA3-CA1 synapses. (2015)
  • Author(s): Yo Shinoda, Chiaki Ishii, Yugo Fukazawa, Tetsushi Sadakata, Takuji Iwasato, Shigeyoshi Itohara, and Teiichi Furuichi.
  • Organizer: Neuroscience 2015.
  • Place of Presentation: シカゴコンベンションセンター(Chicago, US)
  • Year and Date: 2015-10-18
  • Int'l Joint Research
• [Presentation] Understanding neural functions and diseases from brain-derived neurotrophic factor. (2015)
  • Author(s): Yo Shinoda
  • Organizer: 58th Annual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: ソニックシティーホール（埼玉）
  • Year and Date: 2015-09-13
  • Int'l Joint Research
• [Presentation] Possible involvement of the secretion-related protein CAPS2 in regulation of dynorphin, one of the endogenous opioids, secretion. (2015)
  • Author(s): Momoko Katakai, Yo Shinoda, Teiichi Furuichi
  • Organizer: 58th Annual Meeting of the Japanese Society for Neurochemistry.
  • Place of Presentation: ソニックシティーホール（埼玉）
  • Year and Date: 2015-09-13
  • Int'l Joint Research
• [Presentation] Function and expression of the mouse RasGEF1 family proteins. (2015)
  • Author(s): Kenta Hosaka, Yo Shinoda and Teiichi Furuichi
  • Organizer: 58th Annual Meeting of the Japanese Society for Neurochemistry.
  • Place of Presentation: ソニックシティーホール（埼玉）
  • Year and Date: 2015-09-13
  • Int'l Joint Research
• [Presentation] Conditional knockout and optogenetic study on the involvement of the secretion-related protein CAPS1 in oxytocin-associated social and maternal behavior (2015)
  • Author(s): Haruka Minami, Ryosuke Yamaga, Yo Shinoda, Kenji Sakimura, Manabu Abe, and Teiichi Furuichi
  • Organizer: 58th Annual Meeting of the Japanese Society for Neurochemistry.
  • Place of Presentation: ソニックシティーホール（埼玉）
  • Year and Date: 2015-09-11
  • Int'l Joint Research
• [Presentation] A rubber tail task in CAPS2 KO mice: an initial study (2015)
  • Author(s): Makoto Wada, Masakazu Ide, Kaori Yagishita, Momoko Katakai, Yo Shinoda, Teiichi Furuichi, Kenji Kansaku
  • Organizer: 75th Annual Meeting of the Japanese Society for Animal Psychology
  • Place of Presentation: Japan Women’s University (Tokyo, Japan)
  • Year and Date: 2015-09-10
  • Int'l Joint Research
• [Presentation] Brain Transcriptome Database (BrainTx, formerly CDT-DB) Profiling of spatio-temporal gene expression during postnatal development of mouse brain (2015)
  • Author(s): Yo Shinoda, Akira Sato, Tetsushi Sadakata, Hirozumi Nishibe, Noriyuki Morita, Takafumi Inoue, Michisuke Yuzaki, Yoko Yamaguchi and Teiichi Furuichi
  • Organizer: Neuroinformatics 2015
  • Place of Presentation: Pullman Cairns International（オーストラリア）
  • Year and Date: 2015-08-20
  • Int'l Joint Research
• [Presentation] Conditional knockout and optogenetic study on the involvement of the secretion-related protein CAPS1 in oxytocin-associated social and maternal behavior. (2015)
  • Author(s): Haruka Minami, Ryosuke Yamaga,Yo Shinoda, Kenji Sakimura, Manabu Abeo, and Teiichi Furuichi.
  • Organizer: Neuroscience 2015
  • Place of Presentation: Kobe convention center, Japan
  • Year and Date: 2015-07-30
  • Int'l Joint Research
• [Presentation] CAPS1 associates tethering of SVs on the synaptic plasma membrane. (2015)
  • Author(s): Yo Shinoda, Chiaki Ishii, Yugo Fukazawa, Tetsushi Sadakata, Shigeyoshi Itohara, Takuji Iwasato and Teiichi Furuichi.
  • Organizer: Neuroscience 2015
  • Place of Presentation: Kobe convention center, Japan
  • Year and Date: 2015-07-28
  • Int'l Joint Research
• [Presentation] CAPS1 stabilizes docking state of SVs in presynaptic terminal of hippocampal CA3-CA1 synapses. (2015)
  • Author(s): Yo Shinoda
  • Organizer: Satellite meeting of CADM 2015
  • Place of Presentation: KKR Hotel, Tokyo, Japan
  • Year and Date: 2015-07-22
  • Int'l Joint Research