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Electrical synapses between gap-junctionally connected visual cells increase neural spike generation to enhance glutaminergic excitatory synapses from the visual centers of the common marmoset.

Research Project

Project/Area Number 15K08193
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General physiology
Research InstitutionFujita Health University

Principal Investigator

Hidaka Soh  藤田医科大学, 医学部, 講師 (00228735)

Co-Investigator(Kenkyū-buntansha) 海野 修  東邦大学, 理学部, 教授 (70119907)
Research Collaborator Akahori Yasushi  
Kaneko Chiyuki  
Fujita Kimikazu  
Nanbu Atsushi  
Hatanaka Nobuhiko  
Poznanski Ricard Roman  
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords電気シナプス / ギャップ結合 / 閾値下電流 / 同期興奮 / 興奮性シナプス後電位 / シナプス伝達の効率化 / 大脳皮質視覚野 / 神経回路網の成熟 / グルタミン酸シナプス / 興奮性シナプス後電流 / 網膜 / 化学シナプス / 神経伝達物質
Outline of Final Research Achievements

Electrical synapses through gap junctions occurred between excitatory neurons of cerebral cortex of developing animals. Electrical connections between these neurons are expected to control cells' excitation. In this study, we examined cells' excitation by electrical coupling between excitatory neurons. We found gap junctions involving connexin36 in the cerebral visual cortex of adult common marmoset, and between pyramidal cells in cerebral cortex of developing rats. Dual whole-cell patch clamp analysis of pairs of neighboring cells revealed the occurrence of prominent electrotonic transmission between pairs of cells with symmetrical gap junction conductance. Immature cortical pyramidal cells promote neural spiking by synchronous injection of subthrethold currents into pairs of electrically coupled cells. Electrical coupling via electrical synapses between gap-junctionally connected visual neurons appears to increase spike generation to enhance glutaminergic excitatory synaptic outputs.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果として、コモンマーモセットの大脳皮質視覚野の神経細胞間でギャップ結合を介した電気シナプスがあれば、電気シナプスを介したシナプス前的なCa2+スパイクの増大によって、出力の化学シナプスで情報伝達の改善が起こるというシナプス伝達効率化のメカニズムを解明できた。本研究の成果の学術的意義は、霊長類の脳の成長・発達過程における“経験や環境に依存した脳の神経回路の機能的な成熟機構”の比較生理学的な解明に発展する所にある。また本研究の成果の社会的意義として、“ヒトの脳の機能の発達や成熟の発達生理学的な解明”の研究分野に 波及し、本研究は 発展して行くと考えられる。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (24 results)

All 2019 2018 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Invited: 2 results) Remarks (9 results)

  • [Int'l Joint Research] Universiti Sultan Zainal Abidin(マレーシア)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Conflicting effects by antibodies against connexin36 during the action of intracellular cyclic-AMP onto electrical synapses of retinal ganglion cells.2016

    • Author(s)
      Soh Hidaka
    • Journal Title

      J. Integr. Neurosci.

      Volume: 15 Issue: 04 Pages: 571-591

    • DOI

      10.1142/s021963521650031x

    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 電気シナプスを形成した網膜神経節細胞におけるギャップ結合のチャネル開口率の計測2019

    • Author(s)
      日髙 聡、金子 千之、海野 修
    • Organizer
      第124回日本解剖学会総会・全国学術集会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Electrical synapses of visual cortex pyramidal cells and retinal ganglion cells can enhance excitatory synaptic outputs from these neurons.2018

    • Author(s)
      日髙 聡、海野 修
    • Organizer
      第95回 日本生理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 興奮性視覚系中枢ニューロン間の電気シナプスの構造と機能的意義2018

    • Author(s)
      日髙 聡、海野 修
    • Organizer
      第41回 日本神経科学大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Electrical synapses of retinal ganglion cells and visual cortex pyramidal cells can enhance excitatory synapses through synchronous excitation between cells.2018

    • Author(s)
      日 髙  聰,海 野 修
    • Organizer
      第41回 日本神経科学学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 興奮性視覚系中枢ニューロン間の電気シナプスの構造と機能的意義2017

    • Author(s)
      日 髙  聰、海 野  修
    • Organizer
      第40回 日本神経科学大会
    • Place of Presentation
      千葉県千葉市 幕張メッセ
    • Year and Date
      2017-07-20
    • Related Report
      2016 Research-status Report
  • [Presentation] ギャップ結合で連結した興奮性視覚系中枢ニューロン間電気シナプスの機能的意義2017

    • Author(s)
      日 髙  聰、海 野  修
    • Organizer
      第94回 日本生理学会大会
    • Place of Presentation
      静岡県浜松市 アクトシティ浜松
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
  • [Presentation] Electrical synapses of visual cortex neurons and retinal ganglion cells can enhance chemical output excitatory synapses from these neurons.2017

    • Author(s)
      日 髙  聰,海 野 修
    • Organizer
      第40回 日本神経科学学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 視覚系ニューロン間のギャップ結合・電気シナプスの機能的意義2017

    • Author(s)
      日 髙  聰
    • Organizer
      第40回 日本分子生物学会年次大会・ConBio2017
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Electrical synapses of visual cortex pyramidal cells and retinal ganglion cells can enhance excitatory synaptic outputs from these neurons.2017

    • Author(s)
      日 髙  聰,海 野 修
    • Organizer
      第95回 日本生理学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Inhibitory synapses onto retinal ganglion cells in accordance with electrical synapses between gap-junctionally connected retinal amacrine cells.2016

    • Author(s)
      日 髙  聰
    • Organizer
      第39回 日本神経科学大会
    • Place of Presentation
      神奈川県横浜市 パシフィコ横浜
    • Year and Date
      2016-07-20
    • Related Report
      2016 Research-status Report
  • [Presentation] Inhibitory synapses onto retinal ganglion cells in accordance with electrical synapses between gap-junctionally connected retinal amacrine cells.2016

    • Author(s)
      日 髙  聰
    • Organizer
      第39回 日本神経科学大会
    • Place of Presentation
      横浜市 パシフィコ横浜
    • Year and Date
      2016-07-20
    • Related Report
      2015 Research-status Report
  • [Presentation] ギャップ結合形成した網膜アマクリン細胞間の電気シナプスと,アマクリン細胞から網膜神経節細胞への抑制性シナプスの働き.2016

    • Author(s)
      日 髙  聰
    • Organizer
      第93回 日本生理学会大会
    • Place of Presentation
      札幌市コンベンションセンター
    • Year and Date
      2016-03-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Functional differentiation of gap-junctionally connected retinal amacrine cells and modulation of channel opening of their electrical synapses.2015

    • Author(s)
      日 髙  聰
    • Organizer
      第38回 日本神経科学大会
    • Place of Presentation
      神戸市コンベンションセンター
    • Year and Date
      2015-07-28
    • Related Report
      2015 Research-status Report
  • [Remarks] Journal of Neuroscience, research articles

    • URL

      http://www.jneurosci.org/content/24/46/10553.long

    • Related Report
      2018 Annual Research Report 2017 Research-status Report 2016 Research-status Report
  • [Remarks] Journal of Integrative Neuroscience, The editors

    • URL

      https://jin.imrpress.org/EN/column/column137.shtml

    • Related Report
      2018 Annual Research Report
  • [Remarks] Springer link, Pflügers Archiv, articles

    • URL

      https://link.springer.com/article/10.1007%2Fs004240050664

    • Related Report
      2018 Annual Research Report
  • [Remarks] Journal of Integrative Neuroscience, articles

    • URL

      https://jin.imrpress.org/EN/volumn/volumn_8.shtml

    • Related Report
      2018 Annual Research Report
  • [Remarks] Journal of Integrative Neuroscience, editor

    • URL

      http://www.worldscientific.com/page/jin/editorial-board

    • Related Report
      2017 Research-status Report 2016 Research-status Report
  • [Remarks] Springer link, Pflügers Archiv, articles

    • Related Report
      2017 Research-status Report
  • [Remarks] Journal of Integrative Neuroscience, articles

    • URL

      http://www.worldscientific.com/doi/abs/10.1142/S021963521650031X

    • Related Report
      2017 Research-status Report 2016 Research-status Report
  • [Remarks] Springer link, Pflügers Archiv, articles

    • URL

      http://link.springer.com/article/10.1007%2Fs004240050664

    • Related Report
      2016 Research-status Report
  • [Remarks] 藤田保健衛生大学 研究者情報ページ

    • URL

      http://www.fujita-hu.ac.jp/~hnagashi/LDB/open/shidaka.html

    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2020-03-30  

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