| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
First, we observed the effect of cytochalasin D on thermosensitive TRP channel activation stimulated by temperature changes. Activation of TRP channels was not changed by pretreatment of cytochalasin D. Next, we observed the localization of thermosensitive TRP channel. All TRP channels are located on plasma membrane and its localization was not changed by temperature changes. Then, we tried to construct planar lipid bilayer systems. Because, in this system, channel proteins are isolated from various biological resources and reconstituted into an artificial membrane that has a defined lipid composition. We succeeded to observed TRPM5 channel currents by calcium in this system and application of PIP2 regulated its activation. In addition, although we did not observed any temperature-dependent currents in the absence of calcium, we observed temperature-dependent activation and inactivation of these channels in the presence of calcium.
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