| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Piezo1 is a mechanosensitive Ca2+ permeable cation channel that has been reported to be important for mechanosensation in various tissues in our body. However, the physiological role of Piezo1 in the skin was still unknown. To reveal it, first we tried to identify the localization of Piezo1 in the epidermis and found that Piezo1 was predominantly expressed in keratinocytes. Next, we isolated keratinocytes from the superficial layer of the epidermis and succeeded the patch-clamp recordings. We also breed mice to generate the keratinocyte-specific Piezo1 knockout mice. By combining these, future studies should reveal the functional significance of Piezo1 in the epidermal keratinocytes.
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