| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
In this study, we aimed to reveal mechanisms underlying temperature compensation of the mammalian circadian clock. We established a series of mouse embryonic stem cell (ESC) lines with single or multiplex clock gene ablations. ESC-based in vitro circadian clock formation assay reveals that complexed mutations, such as the CKIδ:CKIε:Cry2 mutant, exhibit an additively lengthened circadian period. By using these mutant cells, we also investigated the relation between period-length alteration and temperature compensation. Although CKIδ deficient cells slightly affected the temperature-insensitivity of period-length, we demonstrated that the temperature compensation property is largely maintained in all mutants. These results show that the ESC-based assay system could offer a more systematic and comprehensive approach to the genotype-chronotype analysis of the intracellular circadian clockwork in mammals.
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