| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Flavonoids, which are contained in fruit and vegetables, exhibit wide variety of cytoprotective effects. We previously reported that quercetin exerted various cytoprotective effects via nuclear factor E2-related factor 2 (Nrf2)-heme oxygenase (HO)-1 pathway. In this study, we focused on cell membrane, which is the first line to interact with extracellular environment and plays an important role in cellular signaling, and investigated the involvement of cell membrane changes in quercetin-induced cytoprotective effects. We showed that quercetin changed the formation of lipid rafts by depleting cholesterol from cell membrane. The expression levels of caveolin-1 and Nrf2 localized in cell membrane were also decreased, and caveolin-1-Nrf2 complex was translocated to cytosol and nucleus. These findings suggested that translocation of caveolin-1-Nrf2 complex to nucleus might lead the induction of HO-1.
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