The role of cell membrane in cytoprotective effects of flavonoids
| Project/Area Number |
15K08228
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
川部 勤 名古屋大学, 医学系研究科(保健), 教授 (20378219)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | フラボノイド / 細胞膜 / heme oxygenase-1 / Nrf2 / caveolin-1 / HO-1 |
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| Outline of Final Research Achievements |
Flavonoids, which are contained in fruit and vegetables, exhibit wide variety of cytoprotective effects. We previously reported that quercetin exerted various cytoprotective effects via nuclear factor E2-related factor 2 (Nrf2)-heme oxygenase (HO)-1 pathway. In this study, we focused on cell membrane, which is the first line to interact with extracellular environment and plays an important role in cellular signaling, and investigated the involvement of cell membrane changes in quercetin-induced cytoprotective effects. We showed that quercetin changed the formation of lipid rafts by depleting cholesterol from cell membrane. The expression levels of caveolin-1 and Nrf2 localized in cell membrane were also decreased, and caveolin-1-Nrf2 complex was translocated to cytosol and nucleus. These findings suggested that translocation of caveolin-1-Nrf2 complex to nucleus might lead the induction of HO-1.
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Report
(4 results)
Research Products
(35 results)
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[Journal Article] Modulation of immunological activity on macrophages induced by diazinon.2017
Author(s)
Ogasawara N, Matsushima M, Kawamura N, Atsumi K, Yamaguchi T, Ochi H, Kusatsugu Y, Oyabu S, Hashimoto N, Hasegawa Y, Ueyama J, Kawabe T.
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Journal Title
Toxicology.
Volume: 379
Pages: 22-30
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Involvement of CD40 in the Expression of Bcl62016
Author(s)
Haruka Ochi, Miyoko Matsushima, Yuka Kodera, Yuto Kusatsugu, Kazuko Atsumi, Sayaka Oyabu, Nanako Ogasawara, Kazuya Takemura, Tsutomu Kawabe
Organizer
ICI 2016
Place of Presentation
Melbourne Convention and Exhibition Centre (Australia)
Related Report
Int'l Joint Research
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