micro RNA as the master key for protection of oxidative stress

• Project/Area Number: 15K08244
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General pharmacology
• Research Institution: Teikyo University
• Principal Investigator: Nakaki Toshio 帝京大学, 医学部, 教授 (30164148)
• Co-Investigator(Kenkyū-buntansha): 青山 晃治 帝京大学, 医学部, 准教授 (00420943) ; 押鐘 浩之 帝京大学, 医学部, 助教 (10727283) ; 松村 暢子 帝京大学, 医学部, 助教 (30317698)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: グルタチオン / EAAC1 / GTRAP3-18 / microRNA / タウリン / システイン / GTRAP / glutathione / micro RNA-96-5p / microRNA-96-5p

Outline of Final Research Achievements

Our preliminary studies indicated GTRAP and EAAC1 are inversely regulated by miR96, but there is no miR96-binding site on GTRAP messenger RNA. We speculated the inhibition by miR96 is not achieved by a direct binding to GTRAP but is mediated through another unidentified protein. This protein has been identified by this project.

Research Products

• [Journal Article] Facilitation of yeast-lethal membrane protein production by detoxifying with GFP tagging (2018)
  • Author(s): Oshikane H, Watabe M, Nakaki T.
  • Journal Title: Protein Expr Purif. Volume: 148 Pages: 40
  • DOI: 10.1016/j.pep.2018.03.011
  • URL: https://pure.teikyo.jp/en/publications/cf67bc48-c6a9-4338-aceb-025fbcc427c4
  • Peer Reviewed / Open Access
• [Journal Article] A simple and effective method for detecting precipitated proteins in MALDI−TOF MS (2018)
  • Author(s): Oshikane H, Watabe M, Nakaki T.
  • Journal Title: Anal Biochem. Volume: 546 Pages: 1
  • DOI: 10.1016/j.ab.2018.01.020
  • Peer Reviewed / Open Access
• [Journal Article] GTRAP3-18 regulates food intake and body weight by interacting with pro-opiomelanocortin (2018)
  • Author(s): Kinoshita C, Aoyama K, Nakaki T.
  • Journal Title: FASEB J. Volume: 32 Issue: 1 Pages: 330
  • DOI: 10.1096/fj.201700421r
  • Peer Reviewed
• [Journal Article] Glutathione in cellular Redox homeostasis: Association with the excitatory amino acid carrier 1 (EAAC1) (2015)
  • Author(s): Aoyama, K. Nakaki, T.
  • Journal Title: Molecules Volume: 20 Issue: 5 Pages: 8742
  • DOI: 10.3390/molecules20058742
  • Peer Reviewed / Open Access
• [Presentation] microRNA制御を受けるRNAタンパク質群によるGTRAP3-18の調節 (2017)
  • Author(s): 木下千智、 押鐘浩之、 渡部正彦、 内海 計、 中木 敏夫
  • Organizer: 第90回 日本薬理学会年会
  • Place of Presentation: 長崎市、長崎ブリックホール
  • Year and Date: 2017-03-16