| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Outline of Final Research Achievements |
The current study was performed to identify epigenetic changes associated with the pathogenesis of airway hyperresponsiveness (AHR) in asthma. In bronchial smooth muscles (BSMs) of mice with asthma, a BSM hyperresponsiveness with an up-regulation of RhoA protein was observed. The RhoA up-regulation induced by antigen tended to be augmented in BSMs of the F3 generation compared to those of the parents. A MeDIP-Seq analysis revealed 93 differentially methylated regions of CpG islands between the diseased and control BSMs. However, no change in the CpG methylation of RhoA gene was observed. Microarray analyses revealed 14 and 131 differentially expressed miRNAs and lncRNAs, respectively, in BSMs of the diseased mice. Some of the ncRNAs that might be associated with RhoA translation are also differentially expressed. These findings suggest that wide variety of epigenetic changes, including changes in functional ncRNA expression, in the BSMs are involved in the development of AHR in asthma.
|
