Regulation of human heme oxygenase-1 gene induction by long non-coding RNAs derived from HO-1 gene enhancer region
Project/Area Number |
15K08256
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General medical chemistry
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Research Institution | Tohoku Medical and Pharmaceutical University (2016-2017) Hirosaki University (2015) |
Principal Investigator |
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Project Period (FY) |
2015-10-21 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | Heme / NRF2 / enhancer RNA / HO-1 / ヘムオキシゲナーゼー1 / ノンコーディングRNA / AKR1C1 / ヒ素応答 / ヘムオキシゲナーゼ-1 / 転写制御 |
Outline of Final Research Achievements |
We previously reported that eRNA E2s, a non-coding RNA derived from human heme oxygenase-1 E2 enhancer region, regulates DEM-responsive heme oxygenase-1 gene (HO-1) induction. Whereas it has been documented that most of enhancer RNAs (eRNAs) are cis-acting and modulate the expression of specific gene, we wondered whether eRNA E2s uniquely regulates HO-1 induction or not. Here we found aldo-keto reductase family 1, member C1 gene (AKR1C1) is one of the eRNA E2s-regulated genes. Diethyl maleate (DEM) and arsenite (As)-responsive gene induction of AKR1C1 was attenuated in eRNA E2s knockdown human cells. In addition, we found As-induced Pol II binding to AKR1C1 promoter region is decreased by eRNA E2s knockdown. Interestingly, AKR1C1 is mapped on chromosome 10, though HO-1 and eRNA E2s is on chromosome 22. Thus, our findings indicate that eRNA E2s functions as a trans-acting regulator of gene induction.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Exosomes derived from SW480 colorectal cancer cells promote cell migration in HepG2 hepatocellular cancer cells via the mitogen-activated protein kinase pathway.2016
Author(s)
M. Chiba , N. Watanabe , M. Watanabe , M. Sakamoto , A. Sato , M. Fujisaki , S. Kubota , S. Monzen , A. Maruyama , N. Nanashima , I. Kashiwakura , T. Nakamura .
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Journal Title
International Journal of Oncology
Volume: 48(1)
Issue: 1
Pages: 305-312
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] オートファジー活性化を生かしたレビー小体病治療への応用2016
Author(s)
丹治邦和、三木康生、猪瀬(丸山)敦史、三村純 、松宮朋穂、 森文秋、今泉忠淳、伊東健、若林孝一
Organizer
日本生化学会 東北支部 第 82 回例会・シンポジウム
Place of Presentation
弘前大学医学部基礎大講堂、弘前大学医学部コミュニケーションセンター(青森県・弘前市)
Year and Date
2016-05-21
Related Report
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