Studies on a novel oxidative stress-reducing effect resulting from generation/excretion of sulfated glycolipids

• Project/Area Number: 15K08264
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Shinshu University
• Principal Investigator: Nakajima Takero 信州大学, 学術研究院医学系, 助教 (30581011)
• Co-Investigator(Kenkyū-buntansha): 田中 直樹 信州大学, 学術研究院医学系, 准教授 (80419374)
• Co-Investigator(Renkei-kenkyūsha): AOYAMA Toshifumi 信州大学, 学術研究院医学系, 教授 (50231105) ; KAMIJO Yuji 信州大学, 学術研究院医学系(医学部附属病院), 准教授 (50377636) ; NAKAMURA Kozo 信州大学, 学術研究院農学系, 准教授 (20345763)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: スルファチド / 酸化ストレス / 生体分子 / PPARα

Outline of Final Research Achievements

In previous studies in human patients and mice, we have found that expression of the sulfated glycolipids sulfatides is negatively correlated with the level of oxidative stress. In the present study, we examined the influence of oxidative stress on the expression and metabolism of sulfatides. We treated cultured cell lines with several concentrations of hydrogen peroxides for 24h. The treatment with high concentration of hydrogen peroxides resulted in significant reduction of sulfatide contents. This indicates that oxidative stress directly affects the expression of sulfatides, and that the powerful stress down-regulates it. On the other hand, treatment with the low concentration tended to increase the content of sulfatides. Since several studies have suggested the importance of sulfatide expression in the growth and survival of cells, this change may be involved in the defensive response against oxidative stress.

Research Products

• [Int'l Joint Research] アメリカ国立癌研究所(米国)
• [Journal Article] Decreased Fatty Acid <i>β</i>-Oxidation Is the Main Cause of Fatty Liver Induced by Polyunsaturated Fatty Acid Deficiency in Mice (2017)
  • Author(s): Nakajima T, Yang Y, Lu Y, Kamijo Y, Yamada Y, Nakamura K, Koyama M, Yamaguchi S, Sugiyama E, Tanaka N, Aoyama T
  • Journal Title: The Tohoku Journal of Experimental Medicine Volume: 242 Issue: 3 Pages: 229-239
  • DOI: 10.1620/tjem.242.229
  • NAID: 130005808367
  • ISSN: 0040-8727, 1349-3329
  • Peer Reviewed
• [Journal Article] Age-dependent PPARα activation induces hepatic sulfatide accumulation in transgenic mice carrying the hepatitis C virus core gene. (2016)
  • Author(s): Tian Y, Yang Y, Zhang X, Nakajima T, Tanaka N, Sugiyama E, Kamijo Y, Lu Y, Moriya K, Koike K, Gonzalez FJ, Aoyama T.
  • Journal Title: Glycoconj J Volume: 33 Issue: 6 Pages: 927-936
  • DOI: 10.1007/s10719-016-9703-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Activation of PPARα by Fatty Acid Accumulation Enhances Fatty Acid Degradation and Sulfatide Synthesis. (2016)
  • Author(s): Yang Y, Feng Y, Zhang X, Nakajima T, Tanaka N, Sugiyama E, Kamijo Y, Aoyama T.
  • Journal Title: Tohoku J Exp Med Volume: 240 Issue: 2 Pages: 113-22
  • DOI: 10.1620/tjem.240.113
  • NAID: 130005245903
  • Peer Reviewed