Development of the efficient cardiac differentiation method of pluri-poteint stem cells by using novel chemicals
| Project/Area Number |
15K08270
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Osaka University (2017)
Kyoto University (2015-2016) |
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Principal Investigator |
Minami Itsunari 大阪大学, 医学系研究科, 特任准教授(常勤) (40362537)
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| Co-Investigator(Renkei-kenkyūsha) |
Uesugi Motonari 京都大学, 化学研究所, 教授 (10402926)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 低分子化合物 / 心筋分化誘導 / 多能性幹細胞 / 臨床応用 / 心筋細胞 / 細胞凍結法 |
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| Outline of Final Research Achievements |
We are considering ways to reduce the cost of current differentiation cultures, biological polymers and growth factors for the cardiomyocyte differentiation.
Using a chemical screening, we discovered the compounds KY03-I, which efficiently induce the differentiation of iPS cells to cardiomyocytes. Our differentiation protocol only requires small compounds and amino acids in the culture. The purity of cardiomyocytes is high at more than 90%, and the production efficiencies of cells are remarkably stable. Furthermore, our method has low cost and low risk of virus contamination. It is compatible with a three-dimensional suspension culture system, making it suitable for large scale production of cardiomyocytes.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Impact of extracellular matrix on engraftment and maturation of pluripotent stem cell-derived cardiomyocytes in a rat myocardial infarct model.2017
Author(s)
Ogasawara T, Okano S, Ichimura H, Kadota S, Tanaka Y, Minami I, Uesugi M, Wada Y, Saito N, Okada K, Kuwahara K, Shiba Y
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Journal Title
Sci Rep
Volume: 7
Issue: 1
Pages: 8630-8630
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A synthetic hybrid molecule for the selective removal of human pluripotent stem cells from cell mixtures.2017
Author(s)
Mao, D., Ando, S., Sato, S., Qin, Y., Hirata, N., Katsuda, Y., Kawase, E., Kuo, T.F., Minami, I., Shiba, Y., Ueda, K., Nakatsuji, N., Uesugi, M.
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Journal Title
Angew. Chem. Int. Ed.
Volume: 56
Issue: 7
Pages: 1765-1770
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts.2016
Author(s)
Shiba Y, Gomibuchi T, Seto T, Wada Y, Ichimura H, Tanaka Y, Ogasawara T, Okada K, Shiba N, Sakamoto K, Ido D, Shiina T, Ohkura M, Nakai J, Uno N, Kazuki Y, Oshimura M, Minami I, Ikeda U
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Journal Title
Nature
Volume: 538
Issue: 7625
Pages: 388-391
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Extracellular Recordings of Patterned Human Pluripotent Stem Cell-Derived Cardiomyocytes on Aligned Fibers2016
Author(s)
Li, J.; Minami, I.; Yu, L.; Tsuji, K.; Nakajima, M.; Qiao, J.; Suzuki, M.; Shimono, K.; Nakatsuji, N.; Kotera, H.; Liu, L*.; Chen, Y*.
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Journal Title
Stem cells international
Volume: 2634013
Issue: 1
Pages: 1-9
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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