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A novel AMPK singaling at the intercalated disc in heart

Research Project

Project/Area Number 15K08271
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General medical chemistry
Research InstitutionOsaka University

Principal Investigator

SHINTANI YASUNORI  大阪大学, 生命機能研究科, 准教授 (20712243)

Co-Investigator(Renkei-kenkyūsha) MORISHIMA Keisuke  大阪大学, 大学院工学系研究科, 教授 (60359114)
YALIKUN Yaxiaer  大阪大学, 大学院工学系研究科, 特任助教 (30735064)
YAMAGUCHI Osamu  愛媛大学, 大学院医学系研究科, 教授 (90467580)
TSUKAMOTO Osamu  大阪大学, 大学院医学系研究科, 助教 (80589151)
KATO Hisakazu  大阪大学, 大学院医学系研究科, 助教 (30589312)
Research Collaborator YASHIROGI Shohei  大阪大学, 大学院生命機能研究科, 大学院生
YAZAWA Issei  大阪大学, 大学院生命機能研究科, 大学院生
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsAMPK / 微小管 / 介在板 / メカノストレス / 介在版
Outline of Final Research Achievements

Recently, AMP-activated protein kinase (AMPK) has been reported to reshape cells by regulating cell polarity and division in addition to its canonical role in metabolism. We found that phosphorylated AMPK localized at the intercalated disks in adult mouse heart. We revealed CLIP170 and several other novel molecules as AMPK substrate at the intercalated disc. The inhibition of AMPK or CLIP S311A mutant increased the stability of microtubules near the cell-cell junction in cardiomyocytes. In order to reveal pathophysiological relevance of AMPK-CLIP signal in the heart, we made inducible heart-specific CLIP S311A overexpressing transgenic mice. In the pathological condition that was induced by doxorubicin injection, S311A mice exacerbated cardiac dysfunction with significant tissue fibrosis compared to the control. In conclusion, the regulation of microtubule dynamic instability by AMPK-CLIP signal is important for maintenance of heart function and the pathogenesis of heart diseases.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (7 results)

All 2017 2016 2015

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Alternatively activated macrophages determine repair of the infarcted adult murine heart.2016

    • Author(s)
      Shiraishi M, Shintani Y, Shintani Y, Ishida H, Saba R, Yamaguchi A, Adachi H, Yashiro K, Suzuki K.
    • Journal Title

      J Clin Invest.

      Volume: 126 Issue: 6 Pages: 2151-66

    • DOI

      10.1172/jci85782

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Btg2 is a Negative Regulator of Cardiomyocyte Hypertrophy through a Decrease in Cytosolic RNA.2016

    • Author(s)
      Masumura Y, Higo S, Asano Y, Kato H, Yan Y, Ishino S, Tsukamoto O, Kioka H, Hayashi T, Shintani Y, Yamazaki S, Minamino T, Kitakaze M, Komuro I, Takashima S, Sakata Y.
    • Journal Title

      Sci Rep.

      Volume: 6 Issue: 1 Pages: 28592-28592

    • DOI

      10.1038/srep28592

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Cell Size Critically Determines Initial Retention of Bone Marrow Mononuclear Cells in the Heart after Intracoronary Injection: Evidence from a Rat Model.2016

    • Author(s)
      Campbell NG, Kaneko M, Shintani Y, Narita T, Sawhney V, Coppen SR, Yashiro K, Mathur A, Suzuki K.
    • Journal Title

      PLoS One.

      Volume: 11 Issue: 7 Pages: e0158232-e0158232

    • DOI

      10.1371/journal.pone.0158232

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] The analysis of microtubules dynamic instability by AMPK in cardiomyocytes2017

    • Author(s)
      矢白木 翔平
    • Organizer
      第69回日本細胞生物学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Regulation of microtubules dynamic instability by AMPK in cardiomyocytes2017

    • Author(s)
      矢白木 翔平
    • Organizer
      3rd European Workshop on AMPK
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 心筋細胞におけるAMPKによる微小管の動的不安定性制御の解析2015

    • Author(s)
      矢白木翔平,新谷泰範,高島成二
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸国際展示場
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 心筋特異的な膜タンパク質の同定2015

    • Author(s)
      矢澤一生,矢白木翔平,新谷泰範,高島成二
    • Organizer
      第62回 日本生化学会 近畿支部例会
    • Place of Presentation
      立命館大学びわこ・くさつキャンパス(滋賀県草津市)
    • Year and Date
      2015-05-16
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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