| Project/Area Number |
15K08273
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中込 咲綾 大阪大学, 医学(系)研究科(研究院), その他 (60423894)
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| Co-Investigator(Renkei-kenkyūsha) |
OKUDA SUGURU 大阪大学, 大学院医学系研究科, 助教 (50511846)
NAGAMORI SHUSHI 大阪大学, 大学院医学系研究科, 准教授 (90467572)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | アミノ酸 / mTORC1 / シグナル伝達 / 細胞内シグナル伝達 / 受容体 |
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| Outline of Final Research Achievements |
Amino acids play an important role as signaling molecules to activate mTORC1, a serine/threonine kinase complex which regulates cellular metabolism. However, the detailed molecular mechanisms of amino acid sensing still remain elusive. In this study, we have tried to understand the molecular basis of amino acids sensing in the upstream of mTORC1, especially focusing on how the cells recognize the extracellular amino acids. The experimental protocol for the comparative proteomics analysis using the plasma membrane fractions has been established. Also, the conditions of cell treatments as well as the methods for detection of mTORC1 activity were optimized, to improve the reliability and the throughput of the analyses. The outcomes the research would significantly contribute to identifying the amino acid sensing molecules in the future studies.
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