| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Male carriers of balanced reciprocal translocation are often sterile due to azoospermia or oligozoospermia. While female carriers undergo recurrent miscarriages. The sexual dimorphism in the gametogenesis process is considered as a main contributing factor of these sex differences. However, it has not been fully elucidated yet. In this study, the meiotic processes were analyzed using a reciprocal translocation mouse model. Male model mice had apoptotic cells at the pachytene and the metaphase stages. Spermatocyte spreads showed that the derivative chromosomes were defective in synapsis and often co-localized with the XY body. Immunofluorescence analysis indicated meiotic sex chromosome inactivation (MSCI) failure in these cells, leading to spermatogenic arrest. These results suggest MSCI failure is a cause of sterility of male carriers.
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