Meiotic regulation and its epigenetic analyses of male-female differences using model mice carrying a balanced reciprocal translocation
| Project/Area Number |
15K08334
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human genetics
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| Research Institution | Fujita Health University |
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Principal Investigator |
Tsutsumi Makiko 藤田医科大学, 総合医科学研究所, 助教 (30377907)
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 均衡型染色体相互転座 / 減数分裂 / 不妊 / 習慣流産 / 精子形成 / 染色体転座 |
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| Outline of Final Research Achievements |
Male carriers of balanced reciprocal translocation are often sterile due to azoospermia or oligozoospermia. While female carriers undergo recurrent miscarriages. The sexual dimorphism in the gametogenesis process is considered as a main contributing factor of these sex differences. However, it has not been fully elucidated yet. In this study, the meiotic processes were analyzed using a reciprocal translocation mouse model. Male model mice had apoptotic cells at the pachytene and the metaphase stages. Spermatocyte spreads showed that the derivative chromosomes were defective in synapsis and often co-localized with the XY body. Immunofluorescence analysis indicated meiotic sex chromosome inactivation (MSCI) failure in these cells, leading to spermatogenic arrest. These results suggest MSCI failure is a cause of sterility of male carriers.
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| Academic Significance and Societal Importance of the Research Achievements |
均衡型相互転座の保因者は650人に1人という比較的高頻度で見られる。本研究では遺伝的バックグラウンドの異なる２種類の転座モデルマウスを解析することにより、それぞれの減数分裂時のクロマチン動態やエピジェネティック修飾を明らかにすると共に、妊孕性との関係を理解する手がかりを得た。このことは生殖問題に直面する転座保因者のより正確なリスク算定を将来行うのに役立つと期待される。
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Report
(5 results)
Research Products
(4 results)
