New analysis of triple negative breast cancer by using LentiPlex

• Project/Area Number: 15K08339
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Gunma University
• Principal Investigator: Oyama Tetsunari 群馬大学, 大学院医学系研究科, 教授 (50233622)
• Research Collaborator: YAMANE Arito
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 乳癌 / TNBC / shRNA / CASP-14 / CPA-4 / breast / CASP14 / CPA4 / LentiPlex / IGF2BP3 / GPR124

Outline of Final Research Achievements

The short hairpin RNA (shRNA) library is often used to detect promising　candidates for specific molecular target therapies in cancer. For our study, we　first performed a shRNA library analysis to identify cellular viability-related　genes in breast cancer TNBC and non-TNBC cell lines. Then, we performed a transcriptome　analysis ton select highly expressed genes in TNBC compared with non-TNBC cell　lines. Finally, we used transcriptome analysis data from the public database RefEx to select the specific genes not expressed in normal organs.
From these analyses, we identified four genes as candidates for molecular target therapy in TNBC, one is Caspase-14 and the other is Carboxypeptidase A4. We performed several immunohistochemical analysis, using tissue microarray from breast cancer human surgical specimens and migration assay and proliferation assay, using cell lines. We had several significant data.

Research Products

• [Journal Article] Expression patterns of claudins in patients with triple-negative breast cancer are associated with nodal metastasis and worse outcome. (2017)
  • Author(s): Katayama A, Handa T, Komatsu K, Togo M, Horiguchi J, Nishiyama M, Oyama T.
  • Journal Title: Pathol Int. Volume: 67(8) Issue: 8 Pages: 404-413
  • DOI: 10.1111/pin.12560
  • Peer Reviewed
• [Journal Article] Caspase14 expression is associated with triple negative phenotypes and cancer stem cell marker expression in breast cancer patients (2017)
  • Author(s): Handa Tadashi、Katayama Ayaka、Yokobori Takehiko、Yamane Arito、Horiguchi Jun、Kawabata-Iwakawa Reika、Rokudai Susumu、Bao Pinjie、Gombodorj Navchaa、Altan Bolag、Kaira Kyoichi、Asao Takayuki、Kuwano Hiroyuki、Nishiyama Masahiko、Oyama Tetsunari
  • Journal Title: Journal of Surgical Oncology Volume: 116 Issue: 6 Pages: 706-715
  • DOI: 10.1002/jso.24705
  • Peer Reviewed
• [Journal Article] Stathmin1 expression is associated with aggressive phenotypes and cancer stem cell marker expression in breast cancer patients. (2017)
  • Author(s): Obayashi S, Horiguchi J, Higuchi T, Katayama A, Handa T, Altan B, Bai T, Bao P, Bao H, Yokobori T, Nishiyama M, Oyama T, Kuwano H.
  • Journal Title: Int J Oncol. Volume: 51(3) Issue: 3 Pages: 781-790
  • DOI: 10.3892/ijo.2017.4085
  • Peer Reviewed / Open Access
• [Journal Article] Caspase14 expression is associated with triple negative phenotypes and cancer stem cell marker expression in breast cancer patients (2017)
  • Author(s): Tadashi Handa, Ayaka Katayama, Takehiko Yokobori, Arito Yamane, Jun Horiguchi, Takaaki Fujii, Reika Iwakawa-Kawabata, Shinji Yoshiyama, Susumu Rokudai, Pinjie Bao, Navchaa Gombodorj, Halin Bao, Bolag Altan, Kyoichi Kaira, Takayuki Asao, Hiroyuki Kuwano, Masahiko Nishiyama, and Tetsunari Oyama
  • Journal Title: Journal of Surgical Oncology Volume: in press
  • Peer Reviewed / Open Access
• [Presentation] Clinical significance of new treatment target candidate Carboxypeptidase A4 (CPA4) expression in the triple negative breast cancer (2018)
  • Author(s): 半田正、片山彩香、横堀武彦、山根有人、川端麗香、藤井孝明、佐野孝昭、西山正彦、小山徹也
  • Organizer: 第107回日本病理学会総会（予定）
• [Presentation] Caspase14 expression is associated with triple negative phenotypes and cancer stem cell marker expression in breast cancer patients. (2018)
  • Author(s): Y Ishikawa, T Handa, A Katayama, T Fujii, J Horiguchi, Y Iino, T Fujita, T Oyama
  • Organizer: European Breast Cancer Conference 11
  • Int'l Joint Research
• [Presentation] Clinical significance of IGF2BP3 expression in Triple Negative Breast Cancers (2017)
  • Author(s): 半田正、横堀武彦、片山彩香、山根有人、川端麗香、吉山伸司、堀口淳、佐野孝昭、西山正彦、小山徹也
  • Organizer: 第106会日本病理学会総会
• [Presentation] Triple negative乳癌における新規治療標的候補CASP14発現の臨床的意義 (2016)
  • Author(s): 半田正、横堀武彦、川端麗香、片山彩香、小松恵、山根有人、吉山伸司、堀口淳、西山正彦、小山徹也
  • Organizer: 第２５回乳癌基礎研究会
  • Place of Presentation: 奈良
  • Year and Date: 2016-07-23
• [Presentation] Triple negative乳癌の標的としてshRNA screeningにより 検出されたGPR124の乳癌における発現 (2015)
  • Author(s): 半田正、片山彩香、山根有人、吉山伸司、堀口淳、西山正彦、 小山徹也
  • Organizer: 第２４回乳癌基礎研究会
  • Place of Presentation: スパリゾートハワイアンズ（いわき 福島）
  • Year and Date: 2015-09-05