Histological analysis of ulcerative colitis using human pathology samples and mice deficient in sulfotransferases
| Project/Area Number |
15K08343
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Human pathology
|
|---|
| Research Institution | University of Fukui |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
川島 博人 千葉大学, 大学院薬学研究院, 教授 (50260336)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | 炎症性腸疾患 / 硫酸転移酵素 / 硫酸化糖鎖 |
|---|
| Outline of Final Research Achievements |
Mononuclear cell infiltrates in ulcerative colitis (UC) mucosa are considered to be recruited via high endothelial venule (HEV)-like vessels displaying mucosal addressin cell adhesion molecules 1 (MAdCAM-1), the ligand for integrin, and/or peripheral lymph node addressin (PNAd), an L-selectin ligand. 6-O-sulfation of N-acetylglucosamine in PNAd is catalyzed by N-acetylglucosamine-6-O-sulfotransferase 1 (GlcNAc6ST-1) and GlcNAc6ST-2. To determine the role of 6-O-sulfation on HEV-like vessels in UC, we employed a colitis model using mice deficient in both sulfotransferases (DKO mice). We found that more inflammatory cells were infiltrated in DKO mouse colitis, and the number of MAdCAM-1-positive vessels was increased in DKO mouse colitis. These findings suggest that MAdCAM-1 compensates PNAd function and contributes to the pathogenesis of UC in DKO mice.
|
Report
(4 results)
Research Products
(25 results)
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] High endothelial venule-like vessels and lymphocyte recruitment in diffuse sclerosing variant of papillary thyroid carcinoma2017
Author(s)
Low S, Kanamori R, Sakai Y, Hoshino H, Hirokawa M, Kawashima H, Higuchi K, Imamura Y, Kobayashi M
Organizer
20th Annual San Diego Glycobiology Symposium
Place of Presentation
San Diego, CA, USA
Year and Date
2017-02-03
Related Report
Int'l Joint Research
-
[Presentation] Apical membrane expression of distinct sulfated glycans represents a novel marker of cholangiolocellular carcinoma2017
Author(s)
Hoshino H, Ohta M, Ito M, Uchimura K, Sakai Y, Uehara T, Low S, Fukushima M, Kobayashi M
Organizer
20th Annual San Diego Glycobiology Symposium
Place of Presentation
San Diego, CA, USA
Year and Date
2017-02-03
Related Report
Int'l Joint Research
-
-
-
-
[Presentation] Apical membrane expression of distinct sulfated glycans represents a novel marker of cholangiolocellular carcinoma2016
Author(s)
Hoshino H, Ohta M, Ito M, Uchimura K, Sakai Y, Uehara T, Low S, Fukushima M, Kobayashi M
Organizer
Annual Conference of the Society for Glycobiology
Place of Presentation
New Orleans, LA, USA
Year and Date
2016-11-19
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
