| Project/Area Number |
15K08379
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Nagasaki University |
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Principal Investigator |
MIURA Shiro 長崎大学, 原爆後障害医療研究所, 講師 (80513316)
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| Co-Investigator(Kenkyū-buntansha) |
吉浦 孝一郎 長崎大学, 原爆後障害医療研究所, 教授 (00304931)
中島 正洋 長崎大学, 原爆後障害医療研究所, 教授 (50284683)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 放射線影響研究 / 放射線発癌 / 原爆被爆者 / 腫瘍組織バンク / 遺伝子不安定性 |
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| Outline of Final Research Achievements |
We have been conducting a cohort study of tissue bank for cancers which were freshly resected from A-bomb survivors together with information on the A-bombing and medical data. To assess the quality of RNA in this biobank, we investigated the level of RNA degradation and its correlation with frozen tissue storage period/yield of extracted RNA in each organ. The quality of extracted RNA in biobank was significantly influenced by storage period, site of tissue, normal/tumor tissues, and yield of extracted RNA, suggesting a necessity of careful quality control in each site of tissue. Furthermore, using nucleic acid extraction DNA, we conducted the exhaustive analysis of A-bomb survivors’ lung cancer by aCGH method. It was shown that short-distance group(<2km from hypocenter) were more size and frequency of the region of gene amplification and the deletion in the tumor region than a long-distance group(≧2km) in each lung adenocarcinoma and squamous cell carcinoma.
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