| Project/Area Number |
15K08382
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Kobe University (2016-2017)
Ehime Prefectural University of Health Science (2015) |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SOFUE Tadashi 香川大学, 医学部附属病院, 講師 (80452671)
KUSHIDA Yoshio 香川大学, 医学部附属病院, 講師 (30325352)
IRINO Satoshi 愛媛県立医療技術大学, 保健科学部, 講師 (70634418)
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| Research Collaborator |
TOKUHARA Yasunori 愛媛県立医療技術大学, 保健科学部, 講師 (60746329)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 尿細胞診 / ポドサイト / 糸球体腎炎 / 半月体形成 / WT1 / ボウマン嚢上皮細胞 / Liquid-based cytology / podocyte / マクロファージ / vimentin / CD68 / PODXL / 尿中ポドサイト / Lquid-based cytology / 酵素抗体法 |
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| Outline of Final Research Achievements |
The purpose of this study was to assess the relationship between urinary WT1-positive cells (podocytes and parietal epithelial cells) and WT1-positive cells in renal biopsy to investigate whether urinary WT1-positive cells are useful for detection of crescent formation. Fifty-two patients with kidney disease were investigated (15 cases with crescentic lesion and 37 cases with non-crescentic lesion) for immunoenzyme staining using anti-WT1 antibody for urine cytology and renal biopsy. Numbers of WT1-positive cells in urine and renal biopsy were counted, respectively. The number of urinary WT1-positive cells in patients with crescentic lesion was significantly higher than in patients with non-crescentic lesion. In addition, the best cut-off value to detect patients with crescentic lesions using urinary was 5 cells/10mL. The results of our study suggest urinary WT1-positive cells can be used to detect patients with crescentic formation using 5 cells/10mL cutoff value.
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