| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Genetic alterations accumulate in crypt epithelial cells during colorectal carcinogenesis. We focused on the role of stromal tissue surrounding tumor epithelial cells. Crypt and stromal compartments were isolated from both normal and tumor tissues surgically resected from 2 patients with colorectal cancer, and global DNA methylation profiles were compared among these tissues. Different DNA methylation profile between normal and tumor crypts reveled certain genes responsible for colorectal cancer. Interestingly, there were few, but significant numbers of genes that showed different DNA methylation status between normal and tumor stroma. This suggests that alterations of not only epithelial cells, but also stromal cells, play important roles in colorectal carcinogenesis through an epigenetic mechanism.
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