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Analysis of epigenetic alterations in tumor stromal cells and application in cancer diagnosis and treatment.

Research Project

Project/Area Number 15K08383
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Human pathology
Research InstitutionIwate Medical University

Principal Investigator

HABANO WATARU  岩手医科大学, 薬学部, 准教授 (50332979)

Co-Investigator(Renkei-kenkyūsha) Sugai Tamotsu  岩手医科大学, 医学部, 教授 (20187628)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords癌間質 / 腺管分離法 / エピジェネティクス
Outline of Final Research Achievements

Genetic alterations accumulate in crypt epithelial cells during colorectal carcinogenesis. We focused on the role of stromal tissue surrounding tumor epithelial cells. Crypt and stromal compartments were isolated from both normal and tumor tissues surgically resected from 2 patients with colorectal cancer, and global DNA methylation profiles were compared among these tissues. Different DNA methylation profile between normal and tumor crypts reveled certain genes responsible for colorectal cancer. Interestingly, there were few, but significant numbers of genes that showed different DNA methylation status between normal and tumor stroma. This suggests that alterations of not only epithelial cells, but also stromal cells, play important roles in colorectal carcinogenesis through an epigenetic mechanism.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (14 results)

All 2018 2017 2016 2015

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 8 results) Presentation (3 results)

  • [Journal Article] Molecular profiling and genome-wide analysis based on somatic copy number alterations in advanced colorectal cancers2018

    • Author(s)
      Sugai T, Takahashi Y, Eizuka M, Sugimoto R, Fujita Y, Habano W, Otsuka K, Sasaki A, Yamamoto E, Matsumoto T, Suzuki H.
    • Journal Title

      Molecular Carcinogenesis

      Volume: 57 Issue: 3 Pages: 451-461

    • DOI

      10.1002/mc.22769

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of the DNA methylation level of cancer-related genes in colorectal cancer and the surrounding normal mucosa.2017

    • Author(s)
      Sugai T, Yoshida M, Eizuka M, Uesugii N, Habano W, Otsuka K, Sasaki A, Yamamoto E, Matsumoto T, Suzuki H.
    • Journal Title

      Clin Epigenetics.

      Volume: 55 Issue: 1 Pages: 55-55

    • DOI

      10.1186/s13148-017-0352-4

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular alterations in colorectal adenomas and intramucosal adenocarcinomas defined by high-density single-nucleotide polymorphism arrays.2017

    • Author(s)
      Eizuka M, Sugai T, Habano W, Uesugi N, Takahashi Y, Kawasaki K, Yamamoto E, Suzuki H, Matsumoto T.
    • Journal Title

      J Gastroenterol

      Volume: - Issue: 11 Pages: 1158-1168

    • DOI

      10.1007/s00535-017-1317-2

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of molecular alterations in laterally spreading tumors of the colorectum.2017

    • Author(s)
      Sugai T, Habano W, Takagi R, Yamano H, Eizuka M, Arakawa N, Takahashi Y, Yamamoto E, Kawasaki K, Yanai S, Ishida K, Suzuki H, Matsumoto T.
    • Journal Title

      J Gastroenterol

      Volume: 52 Issue: 6 Pages: 715-723

    • DOI

      10.1007/s00535-016-1269-y

    • NAID

      110006550042

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular subtypes of colorectal cancers determined by PCR-based analysis.2017

    • Author(s)
      Sugai T, Eizuka M, Takahashi Y, Fukagawa T, Habano W, Yamamoto E, Akasaka R, Otuska K, Matsumoto T, Suzuki H.
    • Journal Title

      Cancer Sci

      Volume: 108(3) Issue: 3 Pages: 427-434

    • DOI

      10.1111/cas.13164

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular differences in the microsatellite stable phenotype between left-sided and right-sided colorectal cancer.2016

    • Author(s)
      Takahashi Y, Sugai T, Habano W, Ishida K, Eizuka M, Otsuka K, Sasaki A, Matsumoto T, Morikawa T, Unno M, Suzuki H.
    • Journal Title

      Int J Cancer

      Volume: 139 Issue: 11 Pages: 2493-2501

    • DOI

      10.1002/ijc.30377

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Combination of the histone deacetylase inhibitor depsipeptide and 5-fluorouracil upregulates major histocompatibility complex class II and p21 genes and activates caspase-3/7 in human colon cancer HCT-116 cells.2016

    • Author(s)
      Okada K, Hakata S, Terashima J, Gamou T, Habano W, Ozawa S
    • Journal Title

      Oncol Rep

      Volume: 36(4) Issue: 4 Pages: 1875-1885

    • DOI

      10.3892/or.2016.5008

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Clinicopathological and molecular alterations in early gastric cancers with the microsatellite instability-high phenotype2016

    • Author(s)
      Sugimoto R, Sugai T, Habano W, Endoh M, Eizuka M, Yamamoto E, Uesugi N, Ishida K, Kawasaki T, Matsumoto T, Suzuki H.
    • Journal Title

      Int J Cancer

      Volume: 1 Issue: 7 Pages: 1689-97

    • DOI

      10.1002/ijc.29916

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of DNA methylation landscape reveals the roles of DNA methylation in the regulation of drug metabolizing enzymes2015

    • Author(s)
      Habano W, Kawamura K, Iizuka N, Terashima J, Sugai T, Ozawa S.
    • Journal Title

      Clinical Epigenetics

      Volume: 7 Issue: 1 Pages: 105-105

    • DOI

      10.1186/s13148-015-0136-7

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] CYP1A1 and CYP1A2 expression levels are differentially regulated in three-dimensional spheroids of liver cancer cells compared to two-dimensional monolayer cultures2015

    • Author(s)
      Terashima J, Goto S, Hattori H, Hoshi S, Ushirokawa M, Kudo K, Habano W, Ozawa S.
    • Journal Title

      Drug Metab Pharmacokinet.

      Volume: 30 Issue: 6 Pages: 434-440

    • DOI

      10.1016/j.dmpk.2015.10.001

    • NAID

      50014429984

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] A CAR-responsive enhancer element locating approximately 31 kb upstream in the 5'-flanking region of rat cytochrome P450 (CYP) 3A1 gene2015

    • Author(s)
      Gamou T, Habano W, Terashima J, Ozawa S.
    • Journal Title

      Drug Metab Pharmacokinet.

      Volume: 30 Issue: 2 Pages: 188-197

    • DOI

      10.1016/j.dmpk.2014.12.008

    • NAID

      210000171579

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] DNAメチル基転移酵素阻害剤併用によるヒト大腸がん細胞株のイリノテカン感受性増強とアポトーシス関連タンパクの発現変動2016

    • Author(s)
      博多修子、寺島潤、幅野渉、小澤正吾
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2016-10-06
    • Related Report
      2016 Research-status Report
  • [Presentation] AhRの肺がん細胞における遺伝子発現制御の解析2016

    • Author(s)
      神馬瑤子、寺島潤、幅野渉、小澤正吾
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2016-10-06
    • Related Report
      2016 Research-status Report
  • [Presentation] Analysis of DNA methylation landscape reveals the roles of DNA methylation in the regulation of drug metabolizing enzymes2015

    • Author(s)
      幅野渉、寺島潤、小澤正吾
    • Organizer
      日本薬物動態学会第30回年回
    • Place of Presentation
      東京
    • Year and Date
      2015-11-12
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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