| Project/Area Number |
15K08389
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
松山 篤二 産業医科大学, 大学病院, 講師 (80351021)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 骨軟部腫瘍 / 融合遺伝子 / 次世代シークエンス / RT-PCR / FISH / FFPE / 病理診断 / 滑膜肉腫 / ユーイング肉腫 / 粘液型脂肪肉腫 / 次世代シーケンス |
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| Outline of Final Research Achievements |
Our initial attempt to detect tumor type-specific fusion genes by next generation sequencing (NGS) was unsuccessful when we used ordinary formalin-fixed, paraffin-embedded (FFPE) bone and soft tissue tumors. The result suggested that FFPE tumor tissue is not suitable for such a state-of-the-art molecular analysis. We, therefore, subjected snap frozen tumor tissues to NGS, and could identify a few unique fusion genes (i.e. PAX3-MAMAL, etc) that had been unpredictable. Subsequent clinicopathologic and molecular analyses of accumulated tumor samples harboring the identical genetic alternations demonstrated some clinicopathologic diversities in such tumors. Thus, we believe that NGS is a potentially useful molecular technique as a diagnostic adjunct of bone and soft tissue tumors.
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